A Sensitive In vitro Spectrophotometric Hydrogen Peroxide Scavenging Assay using 1,10-Phenanthroline

Abstract

Introduction: Arsenic, an established poisonous metalloid has widespread occurrence in the environment which is posing a constant threat to health and survival of all living organisms. In view of arsenic induced oxidative stress and toxicity, this study focuses on the mitigatory role of thymoquinone, a major active component in the volatile oil of Nigella sativa (commonly known as black cumin); as its nutraceutical value can fulfil the demand of such dietary supplements in high risk population areas. Methods: In the current study, brain preparations of male wistar rats were used to assess different biochemical markers of oxidative stress and genotoxicity. Results: Significant and dose-dependent alterations in the level of enzymatic and biochemical biomarkers of oxidative stress were observed in the As-treated system. However, pre-treatment with thymoquinone brought about significant reduction in the As-induced neurotoxicity. Also, a significant decline in arsenic-induced DNA damage was recorded on pre-treatment with thymoquinone in comet assay. Conclusion: This study proves usefulness of antioxidant potential of thymoquione in mitigating the arsenic induced neurotoxicity.

Distinct Antioxidant Activity of a Common Antidepressant Drug Imipramine

“…The methods used for this study were primarily based on action of imipramine with respect to the known antioxidant compound ascorbic acid. 17 In the first two tests antioxidant capacity of imipramine was distinctly higher than the competent antioxidant ascorbic acid; however, in CUPRAC assay imipramine was less active than ascorbic acid although the reducing power of imipramine gradually increased with higher amounts of the compound. The drug imipramine is structurally similar to flavonoids, whose antioxidant capacity has been evaluated by various researchers.…”

“…18 Imipramine being structurally similar to opens up among many other possibilities a structural modification of the parent molecule to form a more competent antioxidant for routine intake by ailing elderly patients, whose requirement for antioxidant is possibly far more than an adult normal human being. Future studies will focus on the mechanism of action of imipramine on specific free radical scavenging abilities such as hydrogen peroxide, 17 superoxide, hydroxyl radical 19 and such studies are underway.…”

Antioxidants and Anticholinesterase Activities of the Characterized Ethanolic of Ripe Sesoot (Garcinia picrorrhiza Miq.) Fruit Extract (GpKar) and Xanthone

“…Hydrogen peroxide scavenging activity was measured by the modification method of ferrous ammonium sulphate and phenanthroline (Mukhopadhyay, et al, 2016). The reaction of ferrous ammonium sulphate and phenanthroline could form Fe 2+ -tri-phenanthroline complex with the color of orange, but if H2O2 exists in that reaction, Fe 2+ -tri-phenanthroline complex would not be formed, thus scavenger of H2O2 might not form Fe 2+ -tri-phenanthroline complex.…”

The Antidepressant Drug Doxepin: A Promising Antioxidant

“…Following the protocol as described by Mukhopadhy et al [26], the percentage of hydrogen peroxide scavenging activity of doxepin was calculated and it was plotted against the different concentrations of the drug (Fig. 4).…”

“…According to the modified method of determining hydrogen peroxide scavenging activity by Mukhopadhyay et al [26], 1.5 ml of different concentrations of the drug solution (25, 50, 100, 200, 500 µg/ml) were added to 0.25 ml of 1 mM ferrous ammonium sulfate and mixed and then to each tube, 62.5 µL of 5 mM hydrogen peroxide were added, and all of them were then incubated for 5 minutes at room temperature in dark. Following the incubation, 1.5 ml volume of 1,10-phenanthroline monohydrate was pipetted into the reaction mixtures and again kept for 1 minute in dark at room temperature.…”