A Sensitive Fluorescent Assay for Tetracycline Detection Based on Triple-helix Aptamer Probe and Cyclodextrin Supramolecular Inclusion

ZnS quantum dots as fluorescence sensor for quantitative detection of tetracycline

“…MIPs-AA/CQDs molecularly imprinted fluorescence sensor with carbon quantum dots and acrylic acid 1.0-60 0.17 [56] (TAP)/ γ-cyclodextrin triple-helix aptamer probe/cyclodextrin 0.005-0.1 0.0016 [57] Silver nanoparticle-encapsulated halloysite nanolumen 0.05-10 0.0124 [8] Silicon quantum dots 0.0113-1.086 0.0009 [4] Aptamer-labelled fluorescent nanoprobeintegrated shell isolated nanoparticle-enhanced fluorescence spectroscopy 0.2-400 0.0162 [22] 3-Mercaptopropionic acid-ZnS quantum dots 0.025-6 0.00003 This work…”

Fluorescent Aptasensors: Design Strategies and Applications in Analyzing Chemical Contamination of Food

“…Upon target-aptamer binding, the THMS is disassembled and releases the STP, which results in fluorescence quenching due to F-Q proximity. Whereas this example represents a turn-off fluorescence approach, the same principle can be used to design a turn-on assay when F and Q in the STP are replaced by two pyrenes, which are strongly fluorescent only when the dimer is formed at close proximity . Another turn-on approach can be designed using dual THMS .…”

“…Structure-switching fluorescent aptasensors have also been in the focus of TET assay developments. He et al designed a turn-on fluorometric assay for TET based on THMS formed by label-free aptamer and bis-pyrene-labeled STP . TET-aptamer binding caused the formation of pyrene excimers, whose fluorescence could be further enhanced by γ-cyclodextrin to quantify TET within a linear concentration range of 5 to 100 nM and with an LOD of 1.6 nM (∼0.7 ng mL –1 ).…”

“…He et al designed a turn-on fluorometric assay for TET based on THMS formed by label-free aptamer and bis-pyrene-labeled STP. 36 TETaptamer binding caused the formation of pyrene excimers, whose fluorescence could be further enhanced by γ-cyclodextrin to quantify TET within a linear concentration range of 5 to 100 nM and with an LOD of 1.6 nM (∼0.7 ng mL −1 ). By assembling aptamer-pendant DNA tetrahedron nanostructurefunctionalized MBs (Apt-tet MBs) and ROX labeled cDNA (ROX-cDNA), Hong et al developed a fluorescent method for TET detection.…”

“…Whereas this example represents a turn-off fluorescence approach, the same principle can be used to design a turn-on assay when F and Q in the STP are replaced by two pyrenes, which are strongly fluorescent only when the dimer is formed at close proximity. 36 Another turn-on approach can be designed using dual THMS. 37 As shown in Figure 1B(e), two aptamer probes contain fluorophores and quenchers at the terminals, and they can combine with STP to form dumbbellshaped dual THMS structures, with the close proximity of two pairs of F and Q.…”