A Semimicrotechnique for the Estimation of Cholinesterase Activity in Boll Weevils1

Simpson, D. R.; Bull, D. L.; Lindquist, D. A.

doi:10.1093/aesa/57.3.367

Cited by 102 publications

(78 citation statements)

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“…Acid phosphatase (AC-P) and alkaline phosphatase (ALK-P) activities were determined according to the method described by Powell & Smith (1954). The activity of acetylcholine esterase (AchE) was measured according to the method described by Simpson et al (1964). The activity of phenoloxidase was based on the method described by Ishaaya and Casida (1974).…”

Section: Methodsmentioning

confidence: 99%

Toxicity and biochemical impacts of some new insecticide mixtures on cotton leafworm Spodoptera littoralis (Boisd.)

El-Mageed¹,

Shalaby²

2011

Plant Prot. Sci.

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Abd El-Mageed A.E.M., Shalaby S.E.M. (2011): Toxicity and biochemical impacts of some new insecticide mixtures on cotton leafworm Spodoptera littoralis (Boisd.). Plant Protect. Sci., 47: 166-175.The susceptibility to five new insecticide mixtures: chlorosan, feroban, cygron, engeo, and kingbo was studied in the 2 nd and 4 th instar larvae of the cotton leafworm Spodoptera littoralis (Boisd.). The efficiency and residual effects of these compounds against S. littoralis under field conditions were also investigated. Obtained results revealed that feroban was the most effective compared with the other toxicants, while engeo was the least toxic insecticide in both instars after 2 and 5 days from treatment. Data also indicated that feroban had the longest half-life (Lt 50 ) while engeo recorded the shortest one. Biochemical analysis showed that the tested compounds caused pronounced changes in acetyl cholinesterase and phenol oxidase.

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Section: Methodsmentioning

confidence: 99%

Toxicity and biochemical impacts of some new insecticide mixtures on cotton leafworm Spodoptera littoralis (Boisd.)

El-Mageed¹,

Shalaby²

2011

Plant Prot. Sci.

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“…The activity of AchE was measured according to Simpson et al( 1964), using acetylcholine bromide at a level of 6 × 10 -3 as a substrate. The activity present in the homogenate was expressed as μg substrate hydrolyzed/insects/min.…”

Section: Assays Of Enzymes and Total Proteinmentioning

confidence: 99%

Photosensitizing Effects of Hematoporphyrin Dihydrochloride against the Flesh FlyParasarcophaga argyrostoma(Diptera: Sarcophagidae)

Abdelsalam

Korayem

El-Sherbini³

et al. 2014

Florida Entomologist

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“…Determination of trehalase, and invertase enzymes according to the method described by Ishaaya and Swiriski (1976). Acetylcholine esterase was measured according to method described by Simpson et al, (1964). Acid and alkaline phosphtase activities were determined by the method described by Laufer and Schin (1971).…”

Section: Biochemical Studies:-mentioning

confidence: 99%

Toxicological and biochemical studies of Methylamine Avermactin, a new type of bioinsecticide against the cotton leafworm, Spodoptera littoralis (Biosd).

Dahi

El-Sayed²,

El-Barkey

et al. 2009

Egyptian Academic Journal of Biological Sciences. A, Entomology

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Larvicidal efficacy of a new semi-synthetic avermectin derivative Methylamine avermectin (Radical 0.5% EC) was determined against larval instars of the Egyptian cotton leafworm, Spodoptera littoralis (Biosd.) in the laboratory, field and semi field experiments. 2 nd and 4 th instar larvae showed greatest susceptibility to the Radical in the laboratory experiment. The LC 50s values of the 2 nd and 4 th larval instar after 48 hours were 0.005 and 0.008 ppm, respectively. Radical was tested with recommended dosage (200 ml / 100 liter water) in field; it caused 84.6% reduction of pest population up to day 8 post-treatment. On the other hand, the semi field application of the same recommended dose on the 2 nd instar larvae showed general mean 73.6% mortality, 7 days after post-treatment. Also, some biochemical changed in the 4 th instar larvae after 48 hours of treatment with tested bioinsecticide were measured. It's clear from the results that activities of trehalase, invertase and acetylcholine esterase were increased in all treatments. Tested bioinsecticide reduced the activity of alkaline phosphatase at all doses compared to untreated larvae. No significant changes in acid-phosphatase activities were observed at all treatment doses. On studying the effect of esterases isozymes patterns, there were no differences in number and position of esterases isozymes between untreated and treated larvae in the whole larval body tissues although each band different in its concentration. The toxicity of the formulation to some beneficial predators was also evaluated in the field. There was no detectable effect of these bioinsecticide on naturally occurring beneficial species.

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A Semimicrotechnique for the Estimation of Cholinesterase Activity in Boll Weevils1

Cited by 102 publications

References 0 publications

Toxicity and biochemical impacts of some new insecticide mixtures on cotton leafworm Spodoptera littoralis (Boisd.)

Toxicity and biochemical impacts of some new insecticide mixtures on cotton leafworm Spodoptera littoralis (Boisd.)

Photosensitizing Effects of Hematoporphyrin Dihydrochloride against the Flesh FlyParasarcophaga argyrostoma(Diptera: Sarcophagidae)

Toxicological and biochemical studies of Methylamine Avermactin, a new type of bioinsecticide against the cotton leafworm, Spodoptera littoralis (Biosd).

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