Photosensitizing Effects of Hematoporphyrin Dihydrochloride against the Flesh Fly<i>Parasarcophaga argyrostoma</i>(Diptera: Sarcophagidae)

Abdelsalam, Salaheldin A.; Korayem, Ahmed M.; El-Sherbini, E. A.; Abdel-Aal, Abdel-Aal A.; Mohamed, Doaa

doi:10.1653/024.097.0440

Cited by 8 publications

(10 citation statements)

References 29 publications

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“…However, under irradiation, it is expected that many intracellular molecules are modified or even inactivated13233. It is also assumed that esterase may be altered by solar radiation, and therefore it may be working with lower efficiency than in unaltered cells.…”

Section: Resultsmentioning

confidence: 99%

Intracellular mechanisms of solar water disinfection

Castro-Alférez

Polo-López

Fernández‐Ibáñez³

2016

Sci Rep

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Solar water disinfection (SODIS) is a zero-cost intervention measure to disinfect drinking water in areas of poor access to improved water sources, used by more than 6 million people in the world. The bactericidal action of solar radiation in water has been widely proven, nevertheless the causes for this remain still unclear. Scientific literature points out that generation of reactive oxygen species (ROS) inside microorganisms promoted by solar light absorption is the main reason. For the first time, this work reports on the experimental measurement of accumulated intracellular ROS in E. coli during solar irradiation. For this experimental achievement, a modified protocol based on the fluorescent probe dichlorodihydrofluorescein diacetate (DCFH-DA), widely used for oxidative stress in eukaryotic cells, has been tested and validated for E. coli. Our results demonstrate that ROS and their accumulated oxidative damages at intracellular level are key in solar water disinfection.

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Section: Resultsmentioning

confidence: 99%

Intracellular mechanisms of solar water disinfection

Castro-Alférez

Polo-López

Fernández‐Ibáñez³

2016

Sci Rep

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“…CAT knockdown of R. ferrugineus larvae results in significant growth inhibition and larval mortality ( Al-Ayedh et al 2016 ). Treatment with bioinsecticides, such as plant extracts ( Kolawole and Kolawole 2014 ) and hematoporphyrin photoinsecticides ( Abdelsalam et al 2014 ), resulted in SOD inhibition in cowpea storage beetle and flesh fly, respectively. All spinosad concentrations used in the current study also inhibited GST activity in the midgut of R. ferrugineus .…”

Section: Discussionmentioning

confidence: 99%

Spinosad Induces Antioxidative Response and Ultrastructure Changes in Males of Red Palm WeevilRhynchophorus ferrugineus(Coleoptera: Dryophthoridae)

Abdelsalam

Alzahrani²,

Elmenshawy

et al. 2016

J Insect Sci

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The red palm weevil, Rhynchophorus ferrugineus, is of great concern worldwide, especially in the Middle East, where dates are a strategic crop. Despite their ecological hazard, insecticides remain the most effective means of control. A bioinsecticide of bacterial origin, spinosad is effective against several pests, and its efficacy against male R. ferrugineus was assessed in the present study. The antioxidative responses of key enzymes including catalase (CAT), superoxide dismutase (SOD), and glutathione-S-transferase (GST) to spinosad were investigated in the midgut and testes, and the effects of this insecticide on the cell ultrastructure of the midgut, Malpighian tubules, and testes were also determined. The lethal concentration 50 of spinosad was measured at 58.8 ppm, and the insecticide inhibited the activities of CAT, SOD, and GST in the midgut. However, no significant changes in the activities of these enzymes were observed in the testes. Spinosad treatment resulted in concentration-dependent changes in the cellular organelles of the midgut, Malpighian tubules, and testes of R. ferrugineus, and some of these effects were similar to those exerted by other xenobiotics. However, specific changes were observed as a result of spinosad treatment, including an increase in the number and size of concretions in Malpighian tubule cells and the occasional absence of the central pair of microtubules in the axonemes of sperm tails. This study introduces spinosad for potential use as an insecticide within an integrated control program against male red palm weevils. Additionally, the study provides biochemical and ultrastructural evidence for use in the development of bioindicators.

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“…Abdelsalam et al. () demonstrated an important reduction in the detoxification activity of acetylcholine esterase in adult male flesh flies exposed to HPD. This reduction was generated by singlet oxygen and occurred via the oxidation of tryptophan amino acid in the active site of this enzyme (Michaeli & Feitelson, ).…”

Section: Discussionmentioning

confidence: 99%

“…() proposed that an increase in peroxidized lipids in the thorax of flesh fly adults treated with HP‐dihydrochloride (HPD) might be associated with perturbations in the activities of antioxidant enzymes. The activities of superoxide dismutase, catalase, and glutathione S‐transferase decreased in HPD‐treated flesh fly (Abdelsalam et al., ). If this also occurred in medflies, increasing peroxidized lipids in the head and later in the thorax might affect the detoxifying system with consequent accumulation of ROS, eventually affecting the proper functioning of mitochondria.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, a misbalance of the detoxifying system may affect this organ. Abdelsalam et al (2014) proposed that an increase in peroxidized lipids in the thorax of flesh fly adults treated with HP-dihydrochloride (HPD) might be associated with perturbations in the activities of antioxidant enzymes. The activities of superoxide dismutase, catalase, and glutathione S-transferase decreased in HPD-treated flesh fly (Abdelsalam et al, 2014).…”

Section: Figurementioning

confidence: 99%

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Poor geotaxis correlated with haematoporphyrin‐induced peroxidation of brain lipids as a predictor of medfly longevity reduction

Bochicchio

Pérez

Allué

et al. 2017

Entomologia Exp Applicata

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Under illumination conditions, porphyrins generate cytotoxic radicals in cells. Our study evaluated the effects of haematoporphyrin IX (HP IX) in a laboratory population of male Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) during exposure to a low fluence rate (39 μE m−2 s−1) of light. We found that exposing flies to HP IX for at least 5 days was sufficient to cause irreversible damage that led to anticipated death, as also provoked by chronic exposure to the same concentration. To identify early indicators of the accelerated senescence, we analysed both in vitro and in vivo parameters. The thiobarbituric acid reactive substances content in the heads of treated flies revealed a significant increase in lipid hydroperoxides at day 10, whereas this occurred several days later in controls. In addition, a significant decrease in glycogen content was observed at 15 days of age, 5 days before the reduction observed in the control group. This decrease has been associated with a decline in locomotor activity. Differences in the distribution of flies in the rearing flasks were observed, reflecting an impairment of the motility and climbing capacity of HP IX‐treated flies. This finding was also corroborated by a geotactic response assay (a rapid iterative negative geotaxis or RING assay). The results presented here demonstrate that low‐lethal oxidative stress can anticipate the senescence of flies, which can be predicted using a simple and fast behavioural test, such as the RING assay.

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Photosensitizing Effects of Hematoporphyrin Dihydrochloride against the Flesh FlyParasarcophaga argyrostoma(Diptera: Sarcophagidae)

Cited by 8 publications

References 29 publications

Intracellular mechanisms of solar water disinfection

Intracellular mechanisms of solar water disinfection

Spinosad Induces Antioxidative Response and Ultrastructure Changes in Males of Red Palm WeevilRhynchophorus ferrugineus(Coleoptera: Dryophthoridae)

Poor geotaxis correlated with haematoporphyrin‐induced peroxidation of brain lipids as a predictor of medfly longevity reduction

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