A semi-automated in vitro gas production technique for ruminant feedstuff evaluation

Maurício, Rogério Martins; Mould, F.L.; Dhanoa, M. S.; Owen, E.; Channa, K.S.; Theodorou, Michael K.

doi:10.1016/s0377-8401(99)00033-4

Cited by 468 publications

(311 citation statements)

References 10 publications

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“…Foram feitos ensaios de degradabilidade e descrição de cinética de fermentação por meio da técnica in vitro semi-automática de produção de gases (Maurício et al, 1999). O modelo de France et al (1993) foi utilizado para estimar os parâmetros referentes à cinética de produção de gases sendo estes: a produção de gases cumulativa (ml) y, o tempo de incubação t (horas), o total de gases (ml) A, o tempo de colonização T (horas) e a taxa de degradação fracional (h -1 ) µ.…”

Section: Methodsunclassified

Produtividade e valor nutritivo da Brachiaria brizantha cv. Marandu em um sistema silvipastoril

Sousa¹,

Maurício

Gonçalves³

et al. 2007

Arq. Bras. Med. Vet. Zootec.

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Section: Methodsunclassified

Produtividade e valor nutritivo da Brachiaria brizantha cv. Marandu em um sistema silvipastoril

Sousa¹,

Maurício

Gonçalves³

et al. 2007

Arq. Bras. Med. Vet. Zootec.

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“…An anaerobic environment was maintained throughout the experiment, from the inoculum preparation until the incubation step by flushing with CO 2 gas. The gas generated by fermentation and CO 2 released by buffering of VFA produced during the fermentation were measured at 0, 2,5,8,12,18,24,36,48 and 72 h by means of a pressure transducer (SIN-54978; GP:50, Grand Island, NY, USA) (Mauricio et al, 1999), fitted with digital data tracker (Tracker 211; Intertechnology Inc., Toronto, ON, Canada). Bottles were vented after each measurement.…”

Section: In Vitro Fermentationmentioning

confidence: 99%

In vitro digestion and fermentation characteristics of canola co-products simulate their digestion in the pig intestine

Woyengo

Jha

Beltranena

et al. 2016

Animal

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Canola co-products are sources of amino acid and energy in pig feeds, but their fermentation characteristics in the pig intestine are unknown. Thus, we determined the in vitro fermentation characteristics of the canola co-products Brassica juncea solvent-extracted canola meal (JSECM), Brassica napus solvent-extracted canola meal (NSECM), B. napus expeller-pressed canola meal (NEPCM) and B. napus cold-pressed canola cake (NCPCC) in comparison with soybean meal (SBM). Samples were hydrolysed in two steps using pepsin and pancreatin. Subsequently, residues were incubated in a buffer solution with fresh pig faeces as inocula for 72 h to measure gas production. Concentration of volatile fatty acids (VFA) per gram of dry matter (DM) of feedstuff was measured in fermented solutions. Apparent ileal digestibility (AID) and apparent hindgut fermentation (AHF) of gross energy (GE) for feedstuffs were obtained from pigs fed the same feedstuffs. On DM basis, SBM, JSECM, NSECM, NEPCM and NCPCC contained 15, 19, 22, 117 and 231 g/kg ether extract; and 85, 223, 306, 208 and 176 g/kg NDF, respectively. In vitro digestibility of DM (IVDDM) of SBM (82.3%) was greater ( P < 0.05) than that of JSECM (68.5%), NSECM (63.4%), NEPCM (67.5%) or NCPCC (69.8%). The JSECM had greater ( P < 0.05) IVDDM than NSECM. The IVDDM for NSECM was lower ( P < 0.05) than that for NEPCM, which was lower ( P < 0.05) than that for NCPCC. Similarly, AID of GE was greatest for SBM followed by NCPCC, JSECM, NEPCM and then NSECM. Total VFA production for SBM (0.73 mmol/g) was lower ( P < 0.05) than that of JSECM (1.38 mmol/g) or NSECM (1.05 mmol/g), but not different from that of NEPCM (0.80 mmol/g) and NCPCC (0.62 mmol/g). Total VFA production of JSECM was greater ( P < 0.05) than that of NSECM. Total VFA production of NSECM was greater ( P < 0.05) than that of NEPCM or NCPCC, which differed ( P < 0.05). The ranking of feedstuffs for total VFA production was similar to AHF of GE. In conclusion, in vitro fermentation characteristics of canola co-products and SBM simulated their fermentation in the small and large intestine of pigs, respectively. The 30% greater VFA production for JSECM than NSECM due to lower lignified fibre of JSECM indicates that fermentation characteristics differ between canola species. The NSECM had the highest fermentability followed by NEPCM and then NCPCC, indicating that fat in canola co-products can limit their fermentability in the hindgut.

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“…Samples were fermented in vitro according to Mauricio et al (1999), with modifications as described in Ahmed et al (2013), to measure in vitro gas production, dry matter digestibility (IVDMD) and production of VFA (Table 1).…”

Section: Preparation Of Homogenate From Aloe Arborescensmentioning

confidence: 99%

Administration ofAloe arborescenshomogenate to cattle: interaction with rumen fermentation and gut absorption of aloin

Bani

Grossi

Lucini

et al. 2016

Italian Journal of Animal Science

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Aloe has long been used as a traditional medicine for its numerous beneficial properties, which are mainly ascribed to b-polysaccharides and phenolic compounds including anthraquinones, anthrones and chromones. However, few studies on large animals are currently available. The effect of whole leaf Aloe arborescens homogenate on the in vitro rumen fermentative processes was tested using alfalfa hay and barley meal as substrates. The Aloe homogeneate was added at three different concentrations (0.4, 2.0 and 10.0 g L À1 of fermentation liquid). The same homogenate was dosed (200 g) orally and through the rumen cannula to three rumen cannulated heifers and orally to six lactating dairy cows to measure the rumen degradation of aloin and the transfer of aloin from the gut into the blood, respectively. The Aloe homogenate did not affect in vitro rumen fermentations and feed digestibility. The administration of Aloe homogenate did not negatively affect animal feed intake and health neither on the cannulated heifers nor on the dairy cows. Aloin underwent a rapid degradation in the rumen milieu, and became undetectable 2 h after oral dosage. However, when Aloe homogenate was administered to dairy cows, aloin appeared in blood as early as 2 h after administration, reached a maximum after 4 h (6.2 6 5.8 lg L À1 ) and progressively decreased thereafter. These results suggest that Aloe compounds can be absorbed into the blood and encourage the study of Aloe as a potential nutraceutical in ruminants. Further studies should determine the most effective in vivo dosage. ARTICLE HISTORY

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A semi-automated in vitro gas production technique for ruminant feedstuff evaluation

Cited by 468 publications

References 10 publications

Produtividade e valor nutritivo da Brachiaria brizantha cv. Marandu em um sistema silvipastoril

Produtividade e valor nutritivo da Brachiaria brizantha cv. Marandu em um sistema silvipastoril

In vitro digestion and fermentation characteristics of canola co-products simulate their digestion in the pig intestine

Administration ofAloe arborescenshomogenate to cattle: interaction with rumen fermentation and gut absorption of aloin

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