A screening lateral flow immunochromatographic assay for on-site detection of okadaic acid in shellfish products

Lu, Shiying; Lin, Clement; Li, Yansong; Meng, Xian-Mei; Yu, Shiyu; Li, Zhaohui; Li, Le; Ren, Hong-Lin; Liu, Zengshan

doi:10.1016/j.ab.2011.12.039

Cited by 44 publications

(24 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…And the electrophysiological assay which relied on patch clamp limited the application of this assay outside the laboratory and needed a skilled professional, while microelectrode array was low-throughput due to fewer wells for experiments. The comparison of analytical performance of impedance technology and other methods for STX and TTX was listed in Table 1 [15,18,[35][36][37] and 2 [38][39][40], respectively. This biosensor for STX detection had a higher sensitivity than other methods, while the biosensor for TTX had a lower sensitivity.…”

Section: Assessment Of Stx and Ttx By The Cardiomyocyte-based Biosensormentioning

confidence: 99%

A novel and functional assay for pharmacological effects of marine toxins, saxitoxin and tetrodotoxin by cardiomyocyte-based impedance biosensor

Wang

et al. 2015

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

Section: Assessment Of Stx and Ttx By The Cardiomyocyte-based Biosensormentioning

confidence: 99%

A novel and functional assay for pharmacological effects of marine toxins, saxitoxin and tetrodotoxin by cardiomyocyte-based impedance biosensor

Wang

et al. 2015

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

“…Sample analysis using LFIC (Lu et al 2012) One hundred microlitres of the above-mentioned solution was loaded onto LFIC test strips and placed into the sample well, and the results were read after 10 min. When there was no OA or DTX-1 in the sample solution, the colour intensity of the two lines in the control and test regions was the same.…”

Section: Sample Preparationmentioning

confidence: 99%

“…According to LFIC in a previously reported study (Lu et al 2012), colloidal gold test strips could be used to detect OA toxin in the range of 10-50 ng/ml. The qualitative detection limit of 150 μg/kg of sample was close to the EU regulatory limit (160 μg/kg).…”

Section: Comparison Of Elisa Lfic and A Commercial Elisa Kitmentioning

confidence: 99%

Contamination of commercially available seafood by key diarrhetic shellfish poisons along the coast of China

Lin

Liu

Tan³

et al. 2014

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

With the increasing number of outbreaks of food-borne diseases caused by okadaic acid (OA) and its analogue dinophysistoxin-1 (DTX-1), two key diarrhetic shellfish poison (DSP) toxins, OA and DTX-1, have become a serious threat to public health and have attracted significant public attention in China. The aim of our study was to monitor OA and DTX-1 contamination in commercially available seafood and to provide references for tracking these toxins and preventing disease outbreaks. From 2010 to 2012, 40 species were collected from six coastal cities of four inland seas in China. An enzyme-linked immunosorbent assay (ELISA) and a lateral flow immunochromatographic (LFIC) test strip were used to analyse the samples, and the results were further confirmed using a commercially available ELISA kit. The monitoring results indicated that 23 of 40 species were positive for contamination. In addition, 14 of the positive species were determined to be inedible because the content of OA and DTX-1 was above the regulatory limit. Simultaneously, we verified that the digestive glands of shellfish tended to accumulate toxin, in contrast to the flesh. The highest concentrations of OA and DTX-1 were recorded in Scapharca broughtonii, which was collected from Qing Dao, in relation to the other analysed species. Moreover, the Arca family as well as Mytilus galloprovincialis were severely contaminated by OA and its analogue. The above results indicate that some of the commercially available seafood from the coastal cities in China may be inedible due to serious marine toxin contamination. The results of this study might play an important role in protecting consumer health and safety screening of marine products.

show abstract

“…On the other hand, many biochemical analysis platforms or methods combined with smartphone appear one after another in point-of-care test domain due to the high integration of smartphone to processor and diverse sensors [15,16]. These smartphone-based applications distribute in different fields including smartphone-based microscopy [17,18], fluorescent imaging [19], imaging cytometry [20], electrocardiography [21,22], lateral flow assays [23,24], surface plasmon resonance-based sensing [25], electrochemical sensing [26,27], immunoassays [28][29][30], and other applications [31][32][33]. Also, the accessories of smartphone-based system appear one after another to improve detection performance [34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Smartphone-based portable biosensing system using cell viability biosensor for okadaic acid detection

Pan

Wan

et al. 2017

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

A screening lateral flow immunochromatographic assay for on-site detection of okadaic acid in shellfish products

Cited by 44 publications

References 28 publications

A novel and functional assay for pharmacological effects of marine toxins, saxitoxin and tetrodotoxin by cardiomyocyte-based impedance biosensor

A novel and functional assay for pharmacological effects of marine toxins, saxitoxin and tetrodotoxin by cardiomyocyte-based impedance biosensor

Contamination of commercially available seafood by key diarrhetic shellfish poisons along the coast of China

Smartphone-based portable biosensing system using cell viability biosensor for okadaic acid detection

Contact Info

Product

Resources

About