A scheme for mass propagation of Lilium in vitro

Takayama, Shinsaku; Misawa, Masanaru

doi:10.1016/0304-4238(83)90017-1

Cited by 53 publications

(23 citation statements)

References 11 publications

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“…For propagation of species of Lilium using tissue culture, bulblets can be produced directly from the bulb scale (Stimart & Ascher 1978;Takayama & Misawa 1983a) and through callus culture (Sheridan 1968;Simmonds & Cumming 1976;Stimart et al 1980). Takayama & Misawa (1983b) reported that propagation through multiplication of bulbs derived from bulb scales is more advantageous than from callus, in view of chromosomal variation and low regenerating potential of callus.…”

Section: Introductionmentioning

confidence: 99%

A revised scheme for mass propagation of Easter Lily

Priyadarshi

Sen

1992

Plant Cell Tiss Organ Cult

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Lilium longiflorum Thunb., commonly known as Easter Lily is widely propagated by vegetative means for its high ornamental value as a pot plant. Following in vitro technique, mass propagation has been achieved through direct production of bulblets from the explant as well as regeneration from callus. The chromosome analysis of the progeny derived from callus even from long term culture, did not reveal any marked variability in chromosome morphology. The stable nature of callus maintained in modified MS medium in long term culture has been confirmed. Along with rapid growth, the regenerating capacity of calli has been maintained for 3 years of culture in the above medium. Following shake culture, large number of bulblets could be obtained from such differentiated calli within 3-4 weeks. The shake culture technique of calli is ideally suited for securing stable regenerants on a mass scale in this species.

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Section: Introductionmentioning

confidence: 99%

A revised scheme for mass propagation of Easter Lily

Priyadarshi

Sen

1992

Plant Cell Tiss Organ Cult

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“…[3][4][5] In the present report, bulb growth and flowering characteristics of in vitro-propagated bulbs of L. auratum in soil are described.…”

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confidence: 99%

Growth and flowering of in vitro-propagated Lilium auratum bulbs in soil.

Takayama

Ohkawa

1990

Plant tissue culture letters

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The cultivation in soil of bulblets of Lilium auratum propagated by tissue culture was investigated. In vitro-produced bulblets of L. auratum grew well in soil and required 3 growing seasons for normal flowering. Flowering time and flower quality were uniform and the plants revealed no virus symptoms. Abnormal flowers (four br five tepals) were observed when the bulbs were less than 15 g, while all flowers were norma when the bulbs were over 15g. Flower opened within a week and the size distribution of flower tepals revealed a normal distribution curve.

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Section: Discussionmentioning

confidence: 99%

“…Bulb scales have a stronger regenerating ability than callus and the time required for bulblet regeneration from scales is less than from callus [12]. Intermediate callus, from which plantlets usually arise, has been found to have a bearing on the genetic uniformity of propagules in some species [6,10,12]. Bulblet regeneration from initial explants is desirable because it can obviate or reduce the additional subculturing steps that are required when shoots are generated from callus and then need to be rooted.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro propagation techniques have been described for Muscari [2] and for a number of other bulbous plants including Narcissus [4,9,10], Lilium [ 12] and Hyacinthus [5]. A difficulty involved with tissue cultures of some of these species has been that callus may be produced which then gives rise to roots and/or shoots rather than bulblets.…”

Section: Introductionmentioning

confidence: 99%

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