A revised scheme for mass propagation of Easter Lily

Abstract

Lilium longiflorum Thunb., commonly known as Easter Lily is widely propagated by vegetative means for its high ornamental value as a pot plant. Following in vitro technique, mass propagation has been achieved through direct production of bulblets from the explant as well as regeneration from callus. The chromosome analysis of the progeny derived from callus even from long term culture, did not reveal any marked variability in chromosome morphology. The stable nature of callus maintained in modified MS medium in long term culture has been confirmed. Along with rapid growth, the regenerating capacity of calli has been maintained for 3 years of culture in the above medium. Following shake culture, large number of bulblets could be obtained from such differentiated calli within 3-4 weeks. The shake culture technique of calli is ideally suited for securing stable regenerants on a mass scale in this species.

Regeneration of flowering plants from difficile lily protoplasts by means of a nurse culture

“…Next, it requires the formation of the embryo proper, which is achieved upon transfer of the embryogenic cells to the hormone-free medium (here, lily regeneration through these procedures). A number of laboratories have already reported the regeneration of lily plants through organogenesis (Stimart and Ascher 1978;Liu and Burger 1986;Priyadarshi and Sen 1992;ArzateFernandez et al 1997;Nhut 1998), while regeneration of lilies through somatic embryogenesis has also been reported (Tribulato et al 1997;Nakano et al 2000). Tribulato et al (1997) confirmed the bipolarity of regenerants by histological investigation of the differentiation process in Lilium longiflorum.…”

Establishment of genetic fidelity of In vitro-raised Lilium bulblets through rapd markers

“…1). This method is however, considered to be a low-risk method for genetic instability (Takayama and Misawa, 1983;Priyadarshi and Sen, 1992). Further, screening of these in vitro-raised bulblets was done to establish clonal fidelity using RAPD markers.…”

Efficient one-step tissue culture protocol for propagation of endemic plant, Lilium martagon var. cattaniae Vis.

“…Lilium species in particular can be efficiently multiplied by bulblet formation or by somatic embryogenesis (Priyadarshi and Sen, 1992;Niimi et al, 1997;Pelkonen, 1997;Chang et al, 2000). Alternatively, leafy bulb scales regeneration from scale a Data were scored after 6 weeks of culture; b Data followed by different letters are significantly different (P<0.05); c On hormone-free MS medium, the mean number of bulblets per explant was 2.61 ± 0.50 (with leaves 0.06 ± 0.04).…”