Growth and flowering of in vitro-propagated Lilium auratum bulbs in soil.

Takayama, Shinsaku; Ohkawa, Kiyoshi

doi:10.5511/plantbiotechnology1984.7.187

Cited by 7 publications

(4 citation statements)

References 2 publications

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“…The applied increase in the external sucrose concentration thus leads to a considerable rise in the external sucrose level which would not argue for a signal function. The positive effect of sucrose on stem formation in lily has been described earlier (Takayama et al 1982).…”

Section: Discussionsupporting

confidence: 65%

“…Lily bulblets regenerating in vitro on scale segments may undergo the vegetative phase change as they may form stems after planting in soil (Takayama et al 1982). This opens the possibility to study the transition from juvenile to adult in a reduced period of development under controlled culture conditions, and without interference of plant parts apart from the explant.…”

Section: Introductionmentioning

confidence: 99%

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Phase change in lily bulblets regenerated in vitro

Langens-Gerrits¹,

Klerk²,

Croes

2003

Physiologia Plantarum

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During the development of the lily (Lilium), three phases can be distinguished: the juvenile, the vegetative adult and the flowering phase. Juvenile bulblets sprout with one or a few leaves whereas vegetative adult bulblets sprout with a stem with elongated internodes. The transition to the vegetative adult phase was studied in lily (Lilium × cv. Star Gazer) bulblets regenerating on bulb scale segments in vitro. The phase change was marked by the development of a tunica‐corpus structure in the apical meristem which leads to the formation of an actively growing stem primordium. This structure is absent in juvenile bulblets. Juvenile bulblets first developed competence for phase change during a culture period of at least 6 weeks at 25°C. Subsequent induction of the phase change occurred during a period of 2 weeks at lower temperature (15°C). A major factor influencing phase transition was bulblet weight. Small bulblets never formed a stem whereas large bulblets always formed a stem under inducing conditions. Large bulblets more often formed a stem than small ones but the relation between bulb growth and phase transition was not absolute. A high sucrose concentration, a large explant and a prolonged period for competence development stimulated bulb growth but also phase transition independently of growth. Lowering the concentration of MS‐minerals reduced bulb growth but did not affect phase transition. Under these conditions, phase change was correlated with a low phosphorus content.

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Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Phase change in lily bulblets regenerated in vitro

Langens-Gerrits¹,

Klerk²,

Croes

2003

Physiologia Plantarum

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“…Advantages of increased bulblet size might be offset by adverse effects on dormancy as a relationship between sucrose supply and bulblet dormancy has been demonstrated for Lilium species (Takayama et al, 1982, Aguettaz et al, 1990. Squires et al (1991) reported prolongation of Narcissus bulblet dormancy when sucrose supply was increased.…”

Section: Discussionmentioning

confidence: 94%

Narcissus bulblet formation in vitro: effects of carbohydrate type and osmolarity of the culture medium

Staikidou

Watson

Harvey

et al. 2005

Plant Cell Tiss Organ Cult

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Shoot clump cultures of Narcissus cultivars St. Keverne and Hawera were used to investigate the effects of culture medium carbon supply, type of carbohydrate and osmolarity on in vitro bulblet development. Increasing the medium osmolarity using mannitol or sorbitol, which did not act as substrates for growth, failed to stimulate bulblet formation with either cultivar. An exception to this was a relatively small increase in total bulblet dry weight per culture, in the cultivar Hawera only, caused by adding 30 g l )1 sorbitol in combination with 30 g l )1 sucrose. Simultaneously increasing the medium osmolarity and carbon supply using the metabolisable carbohydrate sources, sucrose, glucose, fructose or an equimolar mixture of glucose and fructose stimulated bulblet production, total dry matter accumulation and partitioning into bulblets. At comparable levels of carbon supply up to 19.0 g l )1 , bulblet development of both cultivars was similar with monosaccharide and sucrose media. This indicates that substrate supply is more important for bulblet development than osmolarity of the culture medium. The cultivar Hawera also showed similar responses to monosaccharide and sucrose media supplying 37.9 g C l )1 , despite the high osmolarity of monosaccharide media (c. 650 m Osm kg )1 , equivalent to )1.6 MPa, compared to 380 m Osm kg )1 for sucrose medium). However in St. Keverne total dry matter accumulation and dry weight per bulblet were further stimulated only by increasing the sucrose supply from 19.0 to 37.9 g C l )1 , not by increasing the monosaccharide supply. Implications of the findings for Narcissus micropropagation are discussed.

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“…Considering that the bulb size is a characteristic that is directly proportional to the weight, Takayama and Ohkawa (1990) also observed that the bigger the bulb weight, the greater the length of the stem for Lilium auratum. Asker (2012) showed the importance of the presence of lily bulbs during cultivation, since the removal of bulbs in different weeks after planting seemed to reduce plant growth.…”

Section: Plant Heightmentioning

confidence: 99%

Influence of vernalization and bulb size on the production of lily cut flowers and lily bulbs

Almeida

Barbosa

Grossi

et al. 2017

SCA

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Exposure of bulbs to cold, a physiological phenomenon called vernalization, and bulb size are important factors in the production of lily bulbs and flowers. This study aimed to verify the influence of vernalization of bulbs on flowering cut lily plants, as well as the impact of size and shape of harvest on the production and quality of flowers and bulbs. In turn, the way the stems of the plants used for cutflower production are cropped is of higher importance for the production of new flower bulbs. In this sense, the experiment was conducted in Viçosa, MG, in a greenhouse in a randomized block design, in split splot scheme with three replications, in which the vernalization periods (25, 35 and 45 days at 4 ± 1 C) constituted the plots; bulb sizes (diameters of 3.2-3.8 cm; 2.5-3.2 cm 1.9-and 2.5 cm), subplots and ways to harvest (full harvest of the stem at the required length for the commercial harvest of the flower, commercial stem harvest at the commercial length, maintaining 10cm of stem in the soil; removal of the floral buds as soon as their appearance is observed and harvest at the end of the season), the sub subplots. The bulbs were planted in beds, with 15 x 20 cm spacing. It was evaluated the number of plants that flowered and the number of flowers, the length and the diameter of the floral buds, fresh and dry weights, diameter and plant height as well as number, perimeter and amount of fresh and dry bulbs. There was a decrease in the plant height with the increase of the vernalization period and a reduction of the diameter of the planted bulbs, as well as of the number and the fresh and dry weights of the produced buds. The production of flowers and buds in number, size and weight was directly proportional to the size of the planted bulbs, while the form of harvest with removal of flower buds increased the number, the perimeter and the fresh and dry weights of the buds. Bulbs with diameter between 3.2 -3.8 cm, stored for 25 days in cold chamber enable greater production of flowers and the highest stem height, factors that favor the increase in market value. Key words: Exposure to cold. Flowering. Way of harvest. Lilium longiflorum. ResumoA exposição dos bulbos ao frio, fenômeno fisiológico denominado vernalização, e o tamanho do bulbo são importantes na produção de flores de lírio. Por sua vez, a forma de colheita das hastes das plantas utilizadas para corte de flor é preponderante na produção de bulbos filhos. Este estudo teve como objetivo verificar a influência da vernalização dos bulbos em plantas de lírio em flor, bem como o impacto do tamanho e forma da colheita na produção e qualidade de flores e bulbos. O experimento foi conduzido em Viçosa, MG em casa de vegetação, em blocos casualizados, com parcelas sub-subdivididas, com três repetições, em que os períodos de vernalização (25, 35 e 45 dias, a 4± 1̊ C) constituíram as parcelas; os tamanhos de bulbo (diâmetros de 3,2-3,8 cm; 2,5-3,2 cm e 1,9-2,5 cm), as subparcelas e as formas de colheita (colheita total da haste no ponto de colheita c...

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Growth and flowering of in vitro-propagated Lilium auratum bulbs in soil.

Cited by 7 publications

References 2 publications

Phase change in lily bulblets regenerated in vitro

Phase change in lily bulblets regenerated in vitro

Narcissus bulblet formation in vitro: effects of carbohydrate type and osmolarity of the culture medium

Influence of vernalization and bulb size on the production of lily cut flowers and lily bulbs

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