A Scanning Electron Microscopic Study on Endothelial Cells and Kupffer Cells in Rat Liver Sinusoids

Scanning Electron Microscopy of Human Liver Sinusoids

“…4, 8, 10 The first aim of this study is to demonstrate that the difficulty in studying human livers has thus been technically overcome. The previous SEM observations of the hepatic sinusoidal wall have been done mainly in common laboratory animals: rats (BROOKS and HAGGIS, 1973;MOTTA and PORTER, 1974;MOTTA, 1975;MUTO, 1975;GRISHAM et al, 1976;EGUCHI et al, 1976), guinea pigs (ITOSHIMA et al, 1974) and rabbits and mice (GRISHAM et al, 1975). The previous works showed that the sinusoidal endothelial cell was provided with numerous fenestrations which were located intracellularly (BROOKS and HAGGIS 1973;MOTTA and PORTER, 1974;ITOSHIMA et al, 1974;MUTO, 1975;MOTTA, 1975;GRISHAM et al, 1975GRISHAM et al, , 1976EGUCHI, 1976) and also partly intercellularly (MUTO, 1975;EGUCHI et al, 1976).…”

“…Recent scanning electron microscope (SEM) studies made on the liver demonstrated that this microscope has advantages over the conventional TEM in that a wide en face view of the tortuous liver cell plates and the sinusoidal wall can easily be obtained. Thus, the SEM studies have contributed much to the elucidation of the tridimensional architectures of hepatocytes (MOTTA and PORTER, 1974;VIAL and PORTER, 1975), bile capillaries (GRISHAM et al, 1975;LAYDEN et al, 1975;MOTTA and FUMAGALLI, 1975;EGUCHI et al, 1976;GRISHAM et al, 1976); and sinusoids (BROOKS and HAGGIS, 1973;MOTTA and PORTER, 1974;ITOSHIMA et al, 1974;MOTTA, 1975a, b;MUTO, 1975;GRISHAM et al, 1975GRISHAM et al, , 1976EGUCHI et al, 1976). These investigations, however, have been made by using laboratory animals, while the application of the SEM has not been undertaken for the human liver, because of the difficulties in obtaining a fresh organ or its pieces whose blood should be eliminated by perfusion.…”

The uptake and long-term storage of India ink particles and latex beads by fibroblasts in the dermis and subcutis of mice, with special regard to the non-inflammatory defense reaction by fibroblasts.

“…By scanning as well as transmission electron microscopy, morphological and functional differences between macrophages and reticuloendothelial cells in some reticular tissues have been clarified by T. FujITA (1974, 1978), T. FujITA and KASHIMURA (1981), MOTTA (1975, MUTO (1975MUTO ( , 1976, TAMARU and FujITA (1978), and other authors. Previously, we confirmed that repeated stimulation by foreign erythrocytes did not cause any transformation of endothelial cells to Kupffer cells or hepatic macrophages (TAMARU and FuJITA, 1978).…”

“…Through transmission as well as scanning electron microscopy, it has become clear that both the reticu-*This study was supported by grants from the Ministry of Education, Science and Culture, Japan lum and endothelial cells are quite different from the macrophages in morphology as well as in function (T. FUJITA, 1974, 1978: T. FUJITA and KASHIMURA, 1981MOTTA, 1975;MUTO, 1975MUTO, , 1976TAMARU and FUJITA, 1978, etc. ) in many kinds of reticular tissues.…”

Three-dimensional organization of the collagen fibrillar framework of the human and rat livers.

“…During these last two decades, scanning electron microscopic (SEM) techniques have greatly contributed towards the visualization of the three-dimensional microstructure of the liver (FUJITA, TOKUNAGA and INOUE, 1971;ITOSHIMA et al, 1974;GRISHAM et al, 1975;LAYDEN et al, 1975;MUTo, 1975;MUTO et al, 1977;MOTTA and PORTER, 1974;MOTTA, 1975MOTTA, , 1977MOTTA, , 1978MOTTA, , 1984MOTTA, MUTO and FUJITA, 1978;KESSEL and KARDON, 1979;VONNAHME, 1981, TAKAHASHI-IWANAGA andFUJITA, 1986). However, the threedimensional organization of the collagen fibrils has *This investigation was supported by a grant from the Ministry of Education, Science and Culture, Japan (63570009).…”