Three-dimensional organization of the collagen fibrillar framework of the human and rat livers.

Ohtani, Osamu

doi:10.1679/aohc.51.473

Cited by 54 publications

(49 citation statements)

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“…However, the methods of observation used in these studies did not clearly visualize how the collagen fibrillar framework changes in these diseases. Recently, the three-dimensional structure of the collagen fibrillar framework of the normal rat and human livers was observed by scanning electron microscopy (SEM) after digestion of the parenchymal tissue with 2.5 N NaOH solution (Ohtani 1988). In the present study, the same method was applied to liver specimens taken from patients with chronic active hepatitis (CAR) and viral and alcoholic liver cirrhoses (LC).…”

mentioning

confidence: 99%

Scanning Electron Microscopic Study on the Three-Dimensional Structure of the Collagen Fibrillar Framework in the Chronic Active Hepatitis and Liver Cirrhosis.

Kawasaki

Ogata

1992

Tohoku J. Exp. Med.

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mentioning

confidence: 99%

Scanning Electron Microscopic Study on the Three-Dimensional Structure of the Collagen Fibrillar Framework in the Chronic Active Hepatitis and Liver Cirrhosis.

Kawasaki

Ogata

1992

Tohoku J. Exp. Med.

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“…Even in normal conditions the liver contains considerable quantities of collagen fibrils mainly in Glisson's sheath and in the liver capsule (Ohtani, 1988). These collagen Correspondence to Prof. Tatsuo Sakai, Department of Anatomy, School of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.…”

Section: mentioning

confidence: 99%

“…Under normal conditions the liver contains considerable quantities of collagen fibrils mainly in Glisson's sheath and in the liver capsule, and fibrils are also found in isolation or in bundles in the space of Disse to form sheaths for housing the hepatic sinusoids (Ohtani, 1988). The quantity of collagen fibrils in the normal liver is modest (12n4 mg and 1n3 mg of collagen fibrils\g of wet tissue weight in the human and rat liver, respectively ; Ohtani, 1988), whereas it increases conspicuously in various types of hepatic fibrosis after injury to hepatic tissue.…”

Section: Distribution Of Collagen Fibrils In the Livermentioning

confidence: 99%

Ultrastructural localisation and size distribution of collagen fibrils in Glisson's sheath of rat liver: implications for mechanical environment and possible producing cells

Hosoyamada¹,

Kurihara

Sakai

2000

Journal of Anatomy

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The ultrastructure and size distributions of collagen fibrils in Glisson's sheath were investigated in the rat liver to analyse the mechanical environment around the fibrils and their possible cells of origin. Glisson's sheath was found to contain 2 populations of collagen fibrils with different diameters and distinct localisations, namely fibroblast-associated and bile epithelium-associated. Fibroblast-associated collagen was composed of fibrils arranged in bundles and constituted the majority of the collagen in Glisson's sheath. Bile epithelium-associated collagen was represented by small dispersed groups of fibrils just beneath the basement membrane of the bile duct. The basement membrane of the bile duct was frequently reduplicated into a few or as many as 10 layers of laminae densae, with scattered collagen fibrils between these laminae. The diameters of the fibrils of both groups of collagen increased in relation to the calibre of the bile duct, whereas at any given place in Glisson's sheath bile epithelium-associated collagen fibrils had a smaller diameter compared with those of the fibroblast-associated fibrils. The increment in fibril diameter along the bile duct is considered to be correlated with the increase in mechanical stress acting on Glisson's sheath. The difference in diameter between the 2 populations as well as the incorporation of fibrils between the laminae densae of the basement membrane of the bile duct supports the view that the bile epithelium-associated collagen is produced by the epithelial cells of the bile duct, thus having a different origin from that of fibroblast-associated collagen. These findings provide the first evidence that the epithelial cells of the interlobular bile duct produce fibril-forming collagen. Furthermore, it is suggested that cholestasis stimulates the epithelial cells of interlobular bile duct to increased synthesis of fibril-forming collagen that is also produced by these cells under physiological conditions.

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“…The 2N NaOH method allows to study by SEM the 3-D organization of the collagen network housed in the extracellular space after selective digestion of all tissue and cell components but collagen, as previously successfully revealed in numerous organs, including pancreas [19], liver [18,20], cardiac tissue [21], lymphoid follicles [22], uterus [23], human umbilical cord [24] and placenta [25]. The 6N NaOH method, by removing connective tissues, allows to study by SEM 3-D the cellular distribution [26,27], mainly represented by vessels and trophoblast.…”

mentioning

confidence: 99%

3-D Ultrastructural Distribution of Collagen in Human Placental Villi at Term in Relation to Vascular Tree

Vizza

Correr

Barberini

et al. 2005

Journal of Reproduction and Development

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Abstract. In order to understand the 3-D distribution of collagen in relation to vascularization, chorionic villi of human placentae, belonging to normal pregnancies at term, were studied by scanning electron microscopy (SEM) after alkali maceration techniques, and by transmission electron microscopy (TEM). The villous tree appeared made of an uninterrupted structure of collagen fibres. The collagen fibres connected the chorionic villi axis with their basal plates and organised differently according to the various levels of villous branching. The collagen of stem villi showed copious fibres. The external fibres (facing the villous surface) were arranged mainly longitudinally. The central core of the villi (inner fibres) were arranged concentrically around the wall of the fetal vessels. Both external and internal fibres formed stratified lamellae or small parallel bundles. The inner core of stem villi showed small holes housing capillary spaces. Mature intermediate and terminal villi showed a scarce amount of collagen arranged in thin concentric layer within the villous core, surrounding numerous dilated capillary and sinusoid spaces.These observations demonstrated that the extracellular matrix of human chorionic villi is highly compartmentalised and shows a variable structural 3-D distribution depending on the branching level of the villous tree, such a distribution ensures the most favourable microenvironment for feto-maternal exchanges and it is likely able to provide a modulated support to the developing chorionic fetal vessels and trophoblastic layer as well. Key words: Placenta, Chorionic villi, Extracellular matrix, Collagen, Angiogenesis, Human (J. Reprod. Dev. 51: [433][434][435][436][437][438][439][440][441][442][443] 2005) he main function of the placenta is to support an adequate feto-maternal blood exchange during pregnancy, indeed an adequate nutrient and oxygen supply is essential for normal d e v e l o p m e n t o f t h e e m b r y o [ 1 ] . F r o m a morphological point of view, human placenta consists of the chorionic plate (fetal surface of placenta), the villous trees and the basal plate (maternal surface of placenta) [2][3][4][5]. The villous tree includes stem villi, emerging from the chorionic plate into the intervillous space. From the stem villi arise free villi and anchoring villi. The latter give rise to smaller intermediate and terminal villi that contain enlarged capillary loops and are responsible of the physiological exchange between maternal and fetal blood [1]. The villous stroma of This paper is dedicated to the memory of Prof. P. M. Motta.

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Three-dimensional organization of the collagen fibrillar framework of the human and rat livers.

Cited by 54 publications

References 48 publications

Scanning Electron Microscopic Study on the Three-Dimensional Structure of the Collagen Fibrillar Framework in the Chronic Active Hepatitis and Liver Cirrhosis.

Scanning Electron Microscopic Study on the Three-Dimensional Structure of the Collagen Fibrillar Framework in the Chronic Active Hepatitis and Liver Cirrhosis.

Ultrastructural localisation and size distribution of collagen fibrils in Glisson's sheath of rat liver: implications for mechanical environment and possible producing cells

3-D Ultrastructural Distribution of Collagen in Human Placental Villi at Term in Relation to Vascular Tree

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