Scanning Electron Microscopy of Human Liver Sinusoids

Fine structural and cytochemical aspects of granuloma formation derived from kupffer cells in mice injected with latex particles.

“…WIDMANN and FAHIMI (1975) reported that latex particles larger than 0.8 im in diameter were never taken up by the sinusoidal endothelial cells of the liver, but in our study large particles, 2.0 pm in diameter could be taken up into this type of cell, though not so often, in addition to smaller particles of 0.2 pm in diameter. We wish to emphasize that the hepatic sinusoidal endothelial cell is also involved in cleaning up the blood stream as TAMARU andFUJITA (1978) andFUJITA et al (1983) reported, though this type of cell essentially differs from the Kupffer cell in morphology (WIssE,1972(WIssE, ,1974a(WIssE, ,1977MoTTA,1975;MUTO,1977;MUTO et al, 1977) and in the grade of endocytotic activity (WIDMANN and FAHIMI,1975). It is well known that the Kupffer cell characteristically shows peroxidase activity in the cisternae of its rough endoplasmic reticulum and nuclear envelope (FAHIMI, 1970;WIDMANN et al, 1972;WISSE, 1974a).…”

Three-dimensional organization of the collagen fibrillar framework of the human and rat livers.

“…During these last two decades, scanning electron microscopic (SEM) techniques have greatly contributed towards the visualization of the three-dimensional microstructure of the liver (FUJITA, TOKUNAGA and INOUE, 1971;ITOSHIMA et al, 1974;GRISHAM et al, 1975;LAYDEN et al, 1975;MUTo, 1975;MUTO et al, 1977;MOTTA and PORTER, 1974;MOTTA, 1975MOTTA, , 1977MOTTA, , 1978MOTTA, , 1984MOTTA, MUTO and FUJITA, 1978;KESSEL and KARDON, 1979;VONNAHME, 1981, TAKAHASHI-IWANAGA andFUJITA, 1986). However, the threedimensional organization of the collagen fibrils has *This investigation was supported by a grant from the Ministry of Education, Science and Culture, Japan (63570009).…”

Further electron-microscope studies on the human hepatic sinusoidal wall with special reference to the fat-storing cell.

“…ITO and SHIBASAKI (1968) carried out the first detailed electron microscopic study on the sinusoidal wall in the normal human liver. The present paper represents a second study, dealing with the human hepatic sinusoidal cells-mainly the fine structure of the fat-storing cell (Ito cell) and the Kupffer cell-while refraining from dealing with the sinusoidal endothelial lining, since this has been thoroughly described by MUTO, NISHI and FUJITA (1977) in their excellent scanning and transmission electron microscopic work with biopsy specimens from normal human livers. …”