A ruthenium(II) complex-based lysosome-targetable multisignal chemosensor for in vivo detection of hypochlorous acid

Cao, Liyan; Zhang, Run; Zhang, Wenzhu; Du, Zhongbo; Liu, Chunjun; Ye, Zhiqiang; Song, Bo; Yuan, Jingli

doi:10.1016/j.biomaterials.2015.07.052

Cited by 114 publications

(73 citation statements)

References 66 publications

(49 reference statements)

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“…13). 31 Probe 14 was weakly luminescent because MLCT (metal-to-ligand charge transfer) state was hindered by an efficient PET (photoinduced electron transfer) process from the Fc moiety to the Ru(II) center. The cleavage of the Fc moiety occurred via a HOCl-induced oxidation of a hydrazide-derived linker, resulting in elimination of PET, accompanied by striking photoluminescence (PL) and electrochemiluminescence (ECL) enhancements.…”

Section: The Chlorination Of Thioesters and Amides By Hoclmentioning

confidence: 99%

Recent progress in chromogenic and fluorogenic chemosensors for hypochlorous acid

Yue

Huo

Yin

et al. 2016

Analyst

162

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Due to the biological and industrial importance of hypochlorous acid, the development of optical probes for HOCl has been an active research area. Hypochlorous acid and hypochlorite can oxidize electron-rich analytes with accompanying changes in molecular sensor spectroscopic profiles. Probes for such processes may monitor HOCl levels in the environment or in an organism and via bio-labeling or bioimaging techniques. This review summarizes recent developments in the area of chromogenic and fluorogenic chemosensors for HOCl.

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Section: The Chlorination Of Thioesters and Amides By Hoclmentioning

confidence: 99%

Recent progress in chromogenic and fluorogenic chemosensors for hypochlorous acid

Yue

Huo

Yin

et al. 2016

Analyst

162

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“…We have previously reported that the ruthenium complex has a broad single-photon absorption spectrum from 350 to 550 nm17181920. For in vivo application of deep-tissue imaging, we first evaluated the two-photon absorption spectrum of these two-photon dual imaging probes.…”

Section: Resultsmentioning

confidence: 99%

“…Up to now, however, no such dual-mode probe has been developed for in vivo real-time molecular imaging. We have previously synthesized a transition-metal complex-based sensing platform for detecting cellular GSH and ROS levels in vitro 17181920. This sensing platform consists of three probes, which exhibit favorable photophysical properties including high photostability, selective and quantitative response.…”

mentioning

confidence: 99%

Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury

Wang

Zhang

Bridle

et al. 2017

Sci Rep

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Oxidative stress reflects an imbalance between reactive oxygen species (ROS) and antioxidants, which has been reported as an early unifying event in the development and progression of various diseases and as a direct and mechanistic indicator of treatment response. However, highly reactive and short-lived nature of ROS and antioxidant limited conventional detection agents, which are influenced by many interfering factors. Here, we present a two-photon sensing platform for in vivo dual imaging of oxidative stress at the single cell-level resolution. This sensing platform consists of three probes, which combine the turn-on fluorescent transition-metal complex with different specific responsive groups for glutathione (GSH), hydrogen peroxide (H2O2) and hypochlorous acid (HOCl). By combining fluorescence intensity imaging and fluorescence lifetime imaging, these probes totally remove any possibility of crosstalk from in vivo environmental or instrumental factors, and enable accurate localization and measurement of the changes in ROS and GSH within the liver. This precedes changes in conventional biochemical and histological assessments in two distinct experimental murine models of liver injury. The ability to monitor real-time cellular oxidative stress with dual-modality imaging has significant implications for high-accurate, spatially configured and quantitative assessment of metabolic status and drug response.

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“…41 Compared with nanoprobe, small molecule fluorescent probes are much easier in membrane permeable and diffuse into cells, and then, in 2015, three small molecules based, lysosome-targeted fluorescent probes were reported with a turn-on response model. [42][43][44] However, to the best of our knowledge, there is no report yet describing a small molecule-based ratiometric fluorescent probe for successful detection of intracellular HOCl in lysosome. Therefore, it is urgent to develop a new effective small molecule-based and ratiometric response model fluorescent probe for imaging endogenous and exogenous HOCl in lysosomes with good membrane permeable, fast response and excellent biocompatibility.…”

Section: Introductionmentioning

confidence: 93%

A lysosome-targeted and ratiometric fluorescent probe for imaging exogenous and endogenous hypochlorous acid in living cells

et al. 2016

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Hypochorous acid plays important roles in many physiological and pathological processes. At the cell organelles level, the abnormal concentration of hypochorous acid in the lysosomes causes redox imbalance and the loss of function of the lysosomes. Herein, the first small molecule based, lysosomal-targeted ratiometric fluorescent HOCl probe (Lyso-HA) was synthesized through reasonable design. The new probe was highly selective toward HOCl over other reactive oxygen species and exhibits a large variation (up to 97-fold) in its fluorescence ratio (I585/I450) with good signal resolution. The probe Lyso-HA was membrane-permeable and suitable for ratiometric visualization of exogenous and endogenous HOCl at lysosomes in living cells.

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A ruthenium(II) complex-based lysosome-targetable multisignal chemosensor for in vivo detection of hypochlorous acid

Cited by 114 publications

References 66 publications

Recent progress in chromogenic and fluorogenic chemosensors for hypochlorous acid

Recent progress in chromogenic and fluorogenic chemosensors for hypochlorous acid

Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury

A lysosome-targeted and ratiometric fluorescent probe for imaging exogenous and endogenous hypochlorous acid in living cells

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