A Ricin B-Like Lectin Protein Physically Interacts with TaPFT and Is Involved in Resistance to Fusarium Head Blight in Wheat

Song, Puwen; Zhang, Lufan; Wu, Liuliu; Hu, Haiyan; Liu, Qili; Li, Dongxiao; Hu, Ping; Zhou, Feng; Bu, Ruifang; Wei, Qichao; Yu, Yao; Guan, Yuanyuan; Chen, Eryong; Su, Xiaojia; Huang, Zhongwen; Qiao, Mu; Ru, Zhengang; Li, Chengwei

doi:10.1094/phyto-11-20-0506-r

Cited by 6 publications

(4 citation statements)

References 47 publications

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“…( 39 ) It also regulates resistance against biotrophic PstDC3000 and necrotrophic S. sclerotiorum via oxidative burst and JA signaling pathway activation in transgenic Arabidopsis. ( 40 ) ZmGLP2-1 contains a ricin B-like lectin (RBL) domain, which protects against Fusarium head blight (FHB) caused by Fusarium graminearum ( F. graminearum ) in wheat 87 and other fungal infections. It suggests that these genes protect against various pathogens by regulating membrane biology.…”

Section: Resultsmentioning

confidence: 99%

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Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Ilyas¹,

Ali

Binjawhar

et al. 2023

ACS Omega

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Germin (GER) and germin-like proteins (GLPs) play an important role in various plant processes. Zea mays contains 26 germin-like protein genes ( ZmGLPs ) located on chromosomes 2, 4, and 10; most of which are functionally unexplored. The present study aimed to characterize all ZmGLPs using the latest computational tools. All of them were studied at a physicochemical, subcellular, structural, and functional level, and their expression was predicted in plant development, against biotic and abiotic stresses using various in silico approaches. Overall, ZmGLPs showed greater similarity in their physicochemical properties, domain architecture, and structure, mostly localized in the cytoplasmic or extracellular regions. Phylogenetically, they have a narrow genetic background with a recent history of gene duplication events on chromosome 4. Functional analysis revealed novel enzymatic activities of phosphoglycolate phosphatase, adenosylhomocysteinase, phosphoglycolate phosphatase-like, osmotin/thaumatin-like, and acetohydroxy acid isomeroreductase largely mediated by disulfide bonding. Expression analysis revealed their crucial role in the root, root tips, crown root, elongation and maturation zones, radicle, and cortex with the highest expression being observed during germination and at the maturity levels. Further, ZmGLPs showed strong expression against biotic ( Aspergillus flavus , Colletotrichum graminicola , Cercospora zeina , Fusarium verticillioides , and Fusarium virguliforme ) while limited expression was noted against abiotic stresses. Concisely, our results provide a platform for additional functional exploration of the ZmGLP genes against various environmental stresses.

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Section: Resultsmentioning

confidence: 99%

“… ZmGLP2-1 contains a ricin B-like lectin (RBL) domain, which protects against Fusarium head blight (FHB) caused by Fusarium graminearum (F. graminearum) in wheat and other fungal infections. It suggests that these genes protect against various pathogens by regulating membrane biology.…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Ilyas¹,

Ali

Binjawhar

et al. 2023

ACS Omega

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“…During the infection, the TaRBL expression is upregulated in resistant cultivars and downregulated in the susceptible ones. Furthermore, inducing TaRBL gene silencing in resistant cultivars using stripe mosaic virus resulted in an apparent reduction of FHB resistance [ 70 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide screening of lectin putative genes from Sorghum bicolor L., distribution in QTLs and a probable implications of lectins in abiotic stress tolerance

2022

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Background Sorghum bicolor is one of the most important crops worldwide with the potential to provide resilience when other economic staples might fail against the continuous environmental changes. Many physiological, developmental and tolerance traits in plants are either controlled or influenced by lectins; carbohydrate binding proteins. Hence, we aimed at providing a comprehensive in silico account on sorghum’s lectins and study their possible implication on various desired agronomical traits. Results We have searched sorghum’s genome from grain and sweet types for lectins putative genes that encode proteins with domains capable of differentially binding carbohydrate moieties and trigger various physiological responses. Of the 12 known plant lectin families, 8 were identified regarding their domain architectures, evolutionary relationships, physiochemical characteristics, and gene expansion mechanisms, and they were thoroughly addressed. Variations between grain and sweet sorghum lectin homologs in term of the presence/absence of certain other joint domains like dirigent and nucleotide-binding adaptor shared by APAF-1, R-proteins, and CED-4 (NB-ARC) indicate a possible neofunctionalization. Lectin sequences were found to be preferentially overrepresented in certain quantitative trait loci (QTLs) related to various traits under several subcategories such as cold, drought, salinity, panicle/grain composition, and leaf morphology. The co-localization and distribution of lectins among multiple QTLs provide insights into the pleiotropic effects that could be played by one lectin gene in numerous traits. Conclusion Our study offers a first-time inclusive details on sorghum lectins and their possible role in conferring tolerance against abiotic stresses and other economically important traits that can be informative for future functional analysis and breeding studies.

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“…Besides the aforementioned genes/proteins, lipid transferase protein LTP [ 98 ] and its interacting protein TaRBL [ 115 ], ARF-GEF protein MIN7 [ 112 ], allene oxide synthase gene TaAOSb [ 70 ], histidine-rich calcium-binding protein TaHRC [ 52 ], putative membrane protein WFhb1-1 [ 122 ], wheat-wall associated kinases TaWAK2A-800 [ 121 ], serine hydroxymethyltransferase TaSHMT3A-1 [ 116 ], stearoyl-acyl carrier protein fatty acid desaturase TaSSI2 [ 118 ], Jacalin-related lectins protein TaJRL53 [ 114 ] were all excavated and proved to be positively involved in FHB response in plants ( Table 2 ). In summary, the results mentioned above urged us to determine the pathways by which the FHB-resistant genes were involved in fungus–plant interactions.…”

Section: The Resistance Mechanism Of Wheat To Fhbmentioning

confidence: 99%

Arms Race between the Host and Pathogen Associated with Fusarium Head Blight of Wheat

Chen

Zhou

et al. 2022

Cells

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Fusarium head blight (FHB), or scab, caused by Fusarium species, is an extremely destructive fungal disease in wheat worldwide. In recent decades, researchers have made unremitting efforts in genetic breeding and control technology related to FHB and have made great progress, especially in the exploration of germplasm resources resistant to FHB; identification and pathogenesis of pathogenic strains; discovery and identification of disease-resistant genes; biochemical control, and so on. However, FHB burst have not been effectively controlled and thereby pose increasingly severe threats to wheat productivity. This review focuses on recent advances in pathogenesis, resistance quantitative trait loci (QTLs)/genes, resistance mechanism, and signaling pathways. We identify two primary pathogenetic patterns of Fusarium species and three significant signaling pathways mediated by UGT, WRKY, and SnRK1, respectively; many publicly approved superstar QTLs and genes are fully summarized to illustrate the pathogenetic patterns of Fusarium species, signaling behavior of the major genes, and their sophisticated and dexterous crosstalk. Besides the research status of FHB resistance, breeding bottlenecks in resistant germplasm resources are also analyzed deeply. Finally, this review proposes that the maintenance of intracellular ROS (reactive oxygen species) homeostasis, regulated by several TaCERK-mediated theoretical patterns, may play an important role in plant response to FHB and puts forward some suggestions on resistant QTL/gene mining and molecular breeding in order to provide a valuable reference to contain FHB outbreaks in agricultural production and promote the sustainable development of green agriculture.

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A Ricin B-Like Lectin Protein Physically Interacts with TaPFT and Is Involved in Resistance to Fusarium Head Blight in Wheat

Cited by 6 publications

References 47 publications

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Molecular Characterization of Germin-like Protein Genes in Zea mays (ZmGLPs) Using Various In Silico Approaches

Genome-wide screening of lectin putative genes from Sorghum bicolor L., distribution in QTLs and a probable implications of lectins in abiotic stress tolerance

Arms Race between the Host and Pathogen Associated with Fusarium Head Blight of Wheat

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