A review on induced mutagenesis in soybean

Hm, Khan

doi:10.5897/jco10.004

Cited by 22 publications

(13 citation statements)

References 14 publications

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“…Ethyl methanesulfonate (EMS), N-nitroso-N-methylurea (NMU), and ethyl nitrosourea (ENU) constitute 64 % of all agents used for chemical mutagenesis (reviewed in [ 18 ]). In soybean, EMS is commonly used, and diverse mutant phenotypes have been reported (reviewed in [ 19 ]). Mutation densities of up to 1/140 kb detected by TILLING have been reported in EMS- or NMU-induced mutant population [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing

et al. 2015

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BackgroundFunctions of most genes predicted in the soybean genome have not been clarified. A mutant library with a high mutation density would be helpful for functional studies and for identification of novel alleles useful for breeding. Development of cost-effective and high-throughput protocols using next generation sequencing (NGS) technologies is expected to simplify the retrieval of mutants with mutations in genes of interest.ResultsTo increase the mutation density, seeds of the Japanese elite soybean cultivar Enrei were treated with the chemical mutagen ethyl methanesulfonate (EMS); M2 seeds produced by M1 plants were treated with EMS once again. The resultant library, which consisted of DNA and seeds from 1536 plants, revealed large morphological and physiological variations. Based on whole-genome re-sequencing analysis of 12 mutant lines, the average number of base changes was 12,796 per line. On average, 691 and 35 per line were missense and nonsense mutations, respectively. Two screening strategies for high resolution melting (HRM) analysis and indexed amplicon sequencing were designed to retrieve the mutants; the mutations were confirmed by Sanger sequencing as the final step. In comparison with HRM screening of several genes, indexed amplicon sequencing allows one to scan a longer sequence range and skip screening steps and to know the sequence information of mutation because it uses systematic DNA pooling and the index of NGS reads, which simplifies the discovery of mutants with amino acid substitutions.ConclusionsA soybean mutant library with a high mutation density was developed. A high mutation density (1 mutation/74 kb) was achieved by repeating the EMS treatment. The mutation density of our library is sufficiently high to obtain a plant in which a gene is nonsense mutated. Thus, our mutant library and the indexed amplicon sequencing will be useful for functional studies of soybean genes and have a potential to yield useful mutant alleles for soybean breeding.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-2079-y) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing

et al. 2015

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“…Increasing the variability and adaptability of soybean by mutation breeding, and followed by selection may become one of the methods to solve the problem. Mutation breeding has been used in recent years as an alternative technique in generating new variability and development of crop species and varieties (Khan and Tyagi, 2013). One of the soybean varieties that have high yielding is Grobagan.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, Grobogan varieties have early maturity (76 days) and larger pod (Krisdiana, 2013) Genetic mutations can be induced by chemical mutagenic agents using some mutation reagents (Oladosu et al 2016) and physic mutagens by gamma rays, x-rays, and ultraviolet (Soeranto et al 2001;Aisyah et al 2005;Sreeju et al 2011). Genetic mutations in soybean crops have been shown to increase the germination rate, viability, survival, yield, quality contributing characters such as pod setting, branching, protein, and oil content, also resistant to drought and diseases (Khan and Tyagi, 2013).…”

Section: Introductionmentioning

confidence: 99%

RAPD Analysis for Genetic Variability Detection of Mutant Soybean (<i>Glycine max</i> (L.) Merr)

Wahyudi

Hapsari

Sundari

2020

J.Tropical Biodiversity Biotechnology

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This study aimed to detect and evaluate the genetic mutation from mutagenized soybean by RAPD markers. Soybean seeds of “Grobogan” variety were treated with two different concentrations of EMS (0.5% and 1%) and three incubation times (4, 6 and 8 h). DNA whole-genome was isolated from young leaf seedling with the Qiagen DNeasy Plant Mini Kit. Twenty OPA primers (OPA-1 to OPA-20) were used for DNA amplification. The results showed that EMS treatments successfully generated genetic variation in soybean, which indicated by high values of PIC, EMR, and MI. RAPD primers that effective to detect the mutation were OPA-2, OPA-07, OPA-10, OPA-11, OPA-12, OPA-13, OPA-14, OPA-15, OPA-16, OPA-18 and OPA-20. The band expression of those primers was exhibited a stronger intensity along with increasing of EMS concentration and incubation time used in this study. Treatment of 0.5% EMS in 6 hours incubation was successfully generated soybean mutants with the lowest genetic similarity compared to the wild-type. Thus, this study provides a new approach to generate genetic variability in soybean and has the potential to improve for soybean breeding program.

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“…42, No. 2 (2020) BARAKAT HASSAN AHMED et al compounds which is described as artificial induction mutation (Sakin, 2002;Srivastava et al, 2011;Khan & Tyagi, 2013;Nura et al, 2013;AL-Nuaimi & Al-Shamma, 2014;Khan & Verma, 2015;Okaz et al, 2016;Sakr, 2016;Al-Shamma & Mohammed, 2018).…”

Section: Introductionmentioning

confidence: 99%

Effect of Chemical Mutagens on Some Morphological and Yield Components Traits of Wheat ( Triticum aestivum L)

2020

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T HIS INVESTIGATION was carried out to induce the mutations in bread wheat (Triticum aestivum L) at the Experimental and Research Farm, Faculty of Agriculture, Al-Azhar University. Two wheat geno.types were treated with different concentrations of di methyl sulfoxide and sodium azide (NaN3). The highest grain yield/ plant (81.27g and 80.56g) were obtained from Sids 14 treated with either (di-methyl sulfoxide at 2000ppm) or sodium azide at 6000ppm. The untreated plant Sids 14 gave 39.23g. The variety Sids 14 was more productive than Misr 1 induction of stable promising mutants according to the final results at M 2 especially with respect to high grain yield/ plant. In general, Sids 14 was more response to chemicals treatment for 1000 grains weight than Misr 1 and gave higher 1000 grains weight. Sids 14 di methyl sulfoxide 1 , Sids 14 DMS 2 , Sids 14 Sodium Azide 1 , Sids 14 Azide 2 and Sids 14 Azide 3 gave (58.27,55g). The geno.types Misr 1 Azide 3 (2, 3 and 6) were short but the genotypes Sids 14 DMS 1 (3 and 7) were tall, while the genotypes Sids 14 DMS 1 (5 and 6) were very tall in both M 1 and M 2. In spite of the high 1000 grain weights variety Sids 14 gave the highest grains no./ spike 72, Sids 14 Azide 3 and Sids 14 DMS 1 giving 88.33 and 87.44 grains, respectively. Mutants Sids 14 Azide 3 and Sids 14 DMS 1 surpassed the original plants in grain no. spike by 22.68% and 21.44%, respectively. There was a significant and positive correlation between yield and spike no./ plant, spikelet no./ spike and grain/ spike across both varieties and generations. There was a low correlation coefficient between grain yield/ plant and 1000 grain weights (0.016) and the negative correlation between grain yield/ plant and plant height (cm).

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A review on induced mutagenesis in soybean

Cited by 22 publications

References 14 publications

Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing

Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing

RAPD Analysis for Genetic Variability Detection of Mutant Soybean (<i>Glycine max</i> (L.) Merr)

Effect of Chemical Mutagens on Some Morphological and Yield Components Traits of Wheat ( Triticum aestivum L)

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