RAPD Analysis for Genetic Variability Detection of Mutant Soybean (&lt;i&gt;Glycine max&lt;/i&gt; (L.) Merr)

Wahyudi, Didik; Hapsari, Lia; Sundari, Sundari

doi:10.22146/jtbb.53653

Cited by 18 publications

(21 citation statements)

References 18 publications

(27 reference statements)

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“…Molecular techniques such as molecular marker offers an opportunity to help detect the changes by examining both genetic variants in the population and detect the desired mutants in a more robust way [13]. This molecular marker can served as complements for morphological markers [14]. Molecular markers can reveal differences among irradiated and non-irradiated individual plants at the DNA level and, thus, provide a more direct, reliable and efficient tool for germplasm conservation and management [15].…”

Section: Resultsmentioning

confidence: 99%

Effect of gamma iradiated seeds of Jabon Merah (Neolamarckia macrophylla (Wall.) Bosser) to genetic diversity

Larekeng

Rachmat

Iswanto

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Jabon merah (Neolamarckia macrophylla (Wall.) as one of the potential industrial forest plantations to meet the need for wood that has a moderate level of genetic diversity. This study aims to analyze the morphological and genetic characters and analyze the stability of genes in explants from red jabon seeds induced by gamma rays at doses of 0 gray, 5 gray, 10 gray, 15 gray, 30 gray, 45 gray and 60 gray, 75 gray, 90 gray, 120 gray and 240 gray. The induced seeds were cultured in vitro. The subculture was started with an initialization on MS0 medium and repeatedly cultured. Finally, the LD50 value was determined using curve fit analysis, explant morphological analysis with SPSS, genetic analysis of explants using RAPD markers and SSR/Microsatellite. The DNA analysis with 9 SSR/Microsatellite and RAPD marker primers showed that most of the induction doses produced more fragments than the control.

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Section: Resultsmentioning

confidence: 99%

Effect of gamma iradiated seeds of Jabon Merah (Neolamarckia macrophylla (Wall.) Bosser) to genetic diversity

Larekeng

Rachmat

Iswanto

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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“…A previous study using mutagenic treatment successfully produced polymorphic bands in Chili (C. annuum). Such band polymorphism was due to variation in a band, the disappearance of bands reducing or altering the binding sites of Taq polymerase and appearance of new bands due to DNA structural alteration [43]. The clustering analysis showed two groups, which appear to be based on cultivars and not by treatment, CH, and M-S group (Fig.…”

Section: F Rapd Profilingmentioning

confidence: 94%

Genetic Variation Based on RAPD Profiling and Production Loss of Cayenne Pepper due to Periodic Flooding

Pahlevi¹,

Indriyani²,

Mastuti³

et al. 2021

Int. J. Adv. Sci. Eng. Inf. Technol.

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Cayenne pepper is known as a sensitive plant to water stress, either drought or flooding. However, not many studies on the plant's response to the naturally occurring periodic flooding have been reported to date. This study aimed to determine the agronomic and genetic response of cayenne pepper against periodic flooding and find whether RAPD profile reflects periodic flooding endurance. Three cultivars of cayenne pepper: Cakra Hijau (CH); Mhanu XR (M); and Sret (S) were used. Plants were treated with periodic flooding P0 (one day of flooding followed by two days of drainage), P1 (2 x P0), and P2 (3 x P0), and C as control. A completely randomized design was used for the experiment, and the data obtained were analyzed statistically. Plant height and the number of fruits between the control and every flooding treated plant were significantly different, indicating that periodic flooding caused the delay of stem growth and decreased fruit number of all cultivars. The number of branches was influenced significantly by periodic flooding.In contrast, the plant survival rate showed no significant difference among all treatments. The higher the periodic flooding, the higher the risk of plant death and increased risk of production loss. Jaccard's clustering on RAPD profiling indicated that the group was developed based on cultivar more than periodic flooding. It was concluded that CH differed from others and had better endurance against periodic flooding, made it a right candidate for a breeding program.

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“…A large number of polymorphic can be explained by the use of DNA templates or methods. According to Wahyudi et al (2020a), the ability of the primer to detect the complementary DNA sequence in the DNA template has a significant impact on the power and authenticity of produced DNA bands. Furthermore, the primer's compatibility with the plant DNA sequence of the local maize cultivars is required for DNA amplification.…”

Section: Dna Concentration and Purity Of 8 Local Maize Cultivars From...mentioning

confidence: 99%

Genetic diversity of eight maize (Zea mays) cultivars from East Nusa Tenggara (Indonesia) based on inter simple sequence repeat markers

YANI

CHASANI²,

Daryono³

2022

Biodiversitas

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Abstract. Yani A, Chasani AR, Daryono BS. 2022. Genetic diversity of eight maize Zea mays L. cultivars from East Nusa Tenggara based on inter simple sequence repeat markers. Biodiversitas 23: 4124-4130. East Nusa Tenggara (NTT) is one of Indonesia's island that has many local maize cultivars. However, the existence of this local corn is threatened by the presence of high-yielding maize seeds. Therefore, characterization of local maize cultivars using molecular marker is needed to conserve the germplasm. This study aims to determine the genetic diversity of local maize cultivars (Zea maysL.) from East Nusa Tenggara based on ISSR markers. This research used eight local maize cultivars (Watar Ohaq, Watar Puluq, Wata Bura, Wata Kuma, Wata Bunga, Pena Muti, Boti Pulut, and Latun Wara). Young leaves of maize were used for DNA isolation. Total genomic DNA was isolated using Genomic DNA Mini Kit for Plant (Geneaid). DNA was then amplified using 5 ISSR molecular markers (ISSR-807, ISSR-808, UBC-809, UBC-811, UBC-834). DNA band profiles were scored based on the presence or absence of bands. Genetic variability was analyzed based on the UPGMA method using the MVSP ver application. 3.1. Local maize from NTT has high genetic diversity (similarity index between 0.29-0.65). Local maize cultivars were separated into two main clusters at a similarity index of 0.43 and the grouping pattern was not influenced by the geographic location of local maize origin.

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RAPD Analysis for Genetic Variability Detection of Mutant Soybean (<i>Glycine max</i> (L.) Merr)

Cited by 18 publications

References 18 publications

Effect of gamma iradiated seeds of Jabon Merah (Neolamarckia macrophylla (Wall.) Bosser) to genetic diversity

Effect of gamma iradiated seeds of Jabon Merah (Neolamarckia macrophylla (Wall.) Bosser) to genetic diversity

Genetic Variation Based on RAPD Profiling and Production Loss of Cayenne Pepper due to Periodic Flooding

Genetic diversity of eight maize (Zea mays) cultivars from East Nusa Tenggara (Indonesia) based on inter simple sequence repeat markers

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