A review on amperometric-type immunosensors based on screen-printed electrodes

Abstract

In this brief review, we summarize the recent research activities involved in the development of amperometric-type immunosensors based on screen-printed electrodes (SPEs). We focus on the underlying principle involved in these types of sensors, their fabrication and electrode surface modification. We also discuss the various factors involved in the designing of such immunosensors and how they affect their performances. Finally we provide an insight into the drawbacks associated with these SPEs.

Reduced graphene oxide-Sb2O5 hybrid nanomaterial for the design of a laccase-based amperometric biosensor for estriol

“…The design of novel, highly sensitive, selective and stable electrochemical biosensors devices able to be used for the fast, accurate, reproducible, in situ and low cost analysis of chemical compounds with biomedical, forensic and environmental importance is a challenge in electroanalytical chemistry [1][2][3]. During last years, progresses in nanomaterials chemistry have empowered biosensor technology by providing a great variety of advanced nanomaterials for their use as transduction elements in electrochemical biosensors [4][5][6][7].…”

Electrochemical Biosensors Based on Nanostructured Carbon Black: A Review

“…Table 3 lists the biosensors based on CB reported up to date. Electrochemical biosensors can be constructed exploring different biological events in according to the target analyte and biological recognition element, ranging from biocatalytic assays (enzymatic biosensors) [73] to affinity assays (genosensors and immunosensors) [74,75]. In analyzing Table 3, it is evident that CB has been mainly applied for enzymatic biosensors.…”

Recent developments, characteristics and potential applications of screen-printed electrodes in pharmaceutical and biological analysis

“…The modification of the SPEs with Abs to produce immunosensors has received considerable attention, since they are extremely selective and sensitivity platforms, having excellent limits of detection and enabling the use of small volumes of samples [32]. On the development of the Abs-modified SPEs, several parameters must be evaluated and optimized, such as the interaction or the binding mechanism between the analyte and the electrode surface to increase the specificity, sensitivity and stability of the immunosensor [34]. Various strategies have been reported for the detection of several drugs and biological compounds by immunosensors based on SPEs (Table 4).…”

“…At the redox potential, ions are released or adsorbed onto the interacting liquid-solid interface, which results in electron transfer to or from the electrodes. Electron transfer induces an electrical current flow between the WE and the CE in the electrolytic cell system, producing an amperometric response, frequently analyzed by cyclic voltammetry (CV) current peak, which is directly proportional to the concentration of the compound in the sample [34].…”