A recommended esterification method for gas chromatographic measurement of conjugated linoleic acid

Yamasaki, Masao; Kishihara, Koji; Ikeda, Ikuo; Sugano, Michihiro; Yamada, Koji

doi:10.1007/s11746-999-0109-0

Cited by 56 publications

(44 citation statements)

References 26 publications

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“…c9,c11-CLA, c9,t11-CLA, t9,t11-CLA and t10,c12-CLA were purchased from Matreya, Inc. (State College, PA, USA). Each CLA was converted to corresponding methyl ester with H 2 SO 4 -methanol solution [16]. Each methyl ester was analyzed by capillary gas chromatography (GC).…”

Section: Methodsmentioning

confidence: 99%

Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells

Beppu

Hosokawa

Tanaka

et al. 2006

The Journal of Nutritional Biochemistry

122

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This study compared the growth inhibitory effects of pure conjugated linoleic acid (CLA) isomers (cis(c)9,c11-CLA, c9,trans(t)11-CLA, t9,t11-CLA, and t10,c12-CLA) on human colon cancer cell lines (Caco-2, HT-29, and DLD-1). When Caco-2 cells were incubated up to 72 h with 200 M each isomer even in the presence of 10% fetal bovine serum (FBS), cell proliferation was inhibited by all CLA isomers in a time dependent manner. The strongest inhibitory effect was shown by t9,t11-CLA, followed by t10,c12-CLA, c9,c11-CLA, and c9,t11-CLA, respectively. The strongest effect of t9,t11-CLA was also observed in other colon cancer cell lines (HT-29 and DLD-1). The order of the inhibitory effect of CLA isomer was confirmed in the presence of 1% FBS. CLA isomers supplemented in the culture medium were readily incorporated into the cellular lipids of Caco-2 and changed their fatty acid composition. The CLA contents in cellular lipids were 26.2+2.7% for t9,t11-CLA, 35.9+0.3% for c9,t11-CLA, and 46.3+0.8% for t10,c12-CLA, respectively. DNA fragmentation was clearly recognized in Caco-2 cells treated with t9,t11-CLA. This apoptotic effect of t9,t11-CLA was dose-and time-dependent. DNA fragmentation was also induced by 9c,11t-CLA and t10,c12-CLA. However, fragmentation levels with both isomers were much lower than that with t9,t11-CLA.t9t11-CLA treatment of Caco-2 cells decreased Bcl-2 levels in association with apoptosis, whereas Bax levels remained unchanged. These results suggest that decreased expression of Bcl-2 by t9t11-CLA might increase the sensitivity of cells to lipid peroxidation and to programmed cell death, apoptosis. AbstractThis study compared the growth inhibitory effects of pure conjugated linoleic acid (CLA) isomers (cis(c)9,c11-CLA, c9,trans(t)11-CLA, t9,t11-CLA, and t10,c12-CLA) on human colon cancer cell lines (Caco-2, HT-29, and DLD-1). When Caco-2 cells were incubated up to 72 h with 200 µM each isomer even in the presence of 10% fetal bovine serum (FBS), cell proliferation was inhibited by all CLA isomers in a time dependent manner. The strongest inhibitory effect was shown by t9,t11-CLA, followed by t10,c12-CLA, c9,c11-CLA, and c9,t11-CLA, respectively. The strongest effect of t9,t11-CLA was also observed in other colon cancer cell lines (HT-29 and DLD-1). The order of the inhibitory effect of CLA isomer was confirmed in the presence of 1% FBS. * Manuscript 2CLA isomers supplemented in the culture medium were readily incorporated into the cellular lipids of Caco-2 and changed their fatty acid composition. The CLA contents in cellular lipids were 26.2+2.7% for t9,t11-CLA, 35.9+0.3% for c9,t11-CLA, and 46.3+0.8% for t10,c12-CLA, respectively. DNA fragmentation was clearly recognized in Caco-2 cells treated with t9,t11-CLA. This apoptotic effect of t9,t11-CLA was doseand time-dependent. DNA fragmentation was also induced by 9c,11t-CLA and t10,c12-CLA. However, fragmentation levels with both isomers were much lower than that with t9,t11-CLA. t9t11-CLA treatment of Caco-2 cells decreased Bcl-2 levels in...

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Section: Methodsmentioning

confidence: 99%

Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells

Beppu

Hosokawa

Tanaka

et al. 2006

The Journal of Nutritional Biochemistry

122

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“…Para definir el perfil lipídico de las especies de gramíneas, se realizó la extracción y metilación de los ácidos grasos adaptando las técnicas descritas por Garcés y Mancha (1993) y por Yamasaki et al (1999). Al forraje (50 mg) se le adicionaron 2.150 μl de metanol absoluto, 990 μl de tolueno, 66 μl de ácido sulfúrico al 99,9 %, 1.000 μl de N,N dimetilformamida y 2 ml de n-hexano.…”

Section: -232unclassified

Efecto de la edad de rebrote sobre el perfil de ácidos grasos en gramíneas tropicales

Mojica-Rodríguez¹,

Rincón²,

Fornaguera³

et al. 2017

Corpoica Cienc. Tecnol. Agropecuaria

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<p>Se evaluó el efecto de tres edades de rebrote (tres, seis y nueve semanas) sobre la producción de forraje, calidad nutricional y perfil de ácidos grasos en gramíneas de pastoreo (diez cultivares y un híbrido)y en gramíneas de corte (cuatro cultivares). Se utilizó un diseño de parcelas divididas con bloques al azar, cuya parcela principal fue la especie forrajera y la subparcela, la edad de rebrote. Los ácidos grasos predominantes presentes en las gramíneas de corte y pastoreo fueron el palmítico (C16:0), linoleico (C18:2) y linolénico (C18:3). La concentración de ácidos grasos en el forraje fue afectada por el estado de madurez en las gramíneas de pastoreo y de corte, pero los efectos no fueron similares en todas las especies. Con excepción del pasto M. maximus cv. Tanzania, que presentó un mayor contenido de precursores de ácido linoleico conjugado, las demás especies presentaron contenidos similares de precursores, lo que sugiere que el uso de estas en la alimentación de bovinos en sistemas de doble propósito resultaría en concentraciones similares de ácido linoleico conjugado c9 t11 en la grasa de la leche.</p>

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“…For esterified lipids, artefacts are still produced (because more severe reaction conditions are required), but at a much lower quantity than those arising from the use of BF 3 . 45,59 Some authors showed good results using a traditional base-catalysed methylation, followed by a mild treatment using H 2 SO 4 -methanol. 45 In general, acid-catalysed methods can be used in a direct transesterification or in a two-step methylation incorporating a previous hydrolysis or saponification of the lipids to free fatty acids.…”

Section: Sulphuric Acid (H 2 So 4 ) In Methanolmentioning

confidence: 99%

Derivatization of fatty acids and its application for conjugated linoleic acid studies in ruminant meat lipids

et al. 2005

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Conjugated linoleic acid (c9, t11 CLA) is a dietary fatty acid produced mainly by ruminant animals and exhibits promising health-promoting biological effects. For lipid fatty acid composition analyses, including CLA, lipids must be pre-treated so that the free and esterified fatty acids (triacylglycerols, phospholipids, etc) are available for determination. The most common treatments involve fatty acid methyl ester derivatives from relatively simple chemical reactions, but this becomes complicated when esterification of CLA is involved because of potential changes in its positional and geometrical isomers by reaction with certain reagents. In this review we explain concisely the advantages and disadvantages of the most popular methods (acid-and base-catalysed methods) generally employed for total fatty acid derivatization and their determination on a gas chromatograph. Based on our experiences we put forward the (trimethylsilyl)diazomethane method as an alternative and successful approach for ruminant tissue lipid determinations.

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A recommended esterification method for gas chromatographic measurement of conjugated linoleic acid

Cited by 56 publications

References 26 publications

Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells

Potent inhibitory effect of trans9, trans11 isomer of conjugated linoleic acid on the growth of human colon cancer cells

Efecto de la edad de rebrote sobre el perfil de ácidos grasos en gramíneas tropicales

Derivatization of fatty acids and its application for conjugated linoleic acid studies in ruminant meat lipids

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