A rapidly new-typed detection of norovirus based on F0F1-ATPase molecular motor biosensor

Zhao, Zhuo; Zhang, Jie; Xu, Meiling; Liu, Zhipeng; Wang, Hua; Liu, Ming; Yu, Yanyan; Sun, Li; Zhang, Hui; Wu, Haiyan

doi:10.1007/s12257-015-0384-6

Cited by 9 publications

(6 citation statements)

References 34 publications

(35 reference statements)

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Section: Other Optical Biosensor Methodsmentioning

confidence: 99%

“…This assay could achieve a LOD of 16 fM and a single-molecule sensitivity, since individual vesicles could be visualized by TIRF microscopy [140]. In another study conducted by Connelly et al [141], a fluorescence based microfluidic biosensor was shown to be able to detect FCV with a LOD of 1.6 × 10 5 PFU/mL within 2.5 h. Another fluorescence based biosensor developed by Zhao et al [142] had been shown to be able to detect NV RNA with a limit of quantification of 0.005 ng/mL within 1 h. The F0F1-ATPase molecular motor biosensor was applied to detect NV RNA with a "ε-subunit antibody-streptomycin-biotin-probe" system. The fluorescence intensity change compared to the control showed that the virus was successfully captured and conjugated to the probe [142].…”

Section: Other Optical Biosensor Methodsmentioning

confidence: 99%

“…The F0F1-ATPase molecular motor biosensor was applied to detect NV RNA with a "ε-subunit antibody-streptomycinbiotin-probe" system. The fluorescence intensity change compared to the control showed that the virus was successfully captured and conjugated to the probe [142]. A fluorescence based biosensor developed by Bally et al [140] was able to successfully detect single NoV VLP (GII.4), by incorporating a H type 1 GSL attached lipid bilayer as a substrate support and a GSL contained rhodamine-labeled vesicle to emit a fluorescent signal after total internal reflection fluorescence (TIRF) illumination, as shown in Figure 4.…”

Section: Other Optical Biosensor Methodsmentioning

confidence: 99%

“…In another study conducted by Connelly et al [141], a fluorescence based microfluidic biosensor was shown to be able to detect FCV with a LOD of 1.6 × 10 5 PFU/mL within 2.5 hr. Another fluorescence based biosensor developed by Zhao et al [142] had been shown to be able to detect NV RNA with a limit of quantification of 0.005 ng/mL within 1 hr. The F0F1-ATPase molecular motor biosensor was applied to detect NV RNA with a "ε-subunit antibody-streptomycinbiotin-probe" system.…”

Section: Other Optical Biosensor Methodsmentioning

confidence: 99%

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A Survey of Analytical Techniques for Noroviruses

Liu

Moore

2020

Foods

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As the leading cause of acute gastroenteritis worldwide, human noroviruses (HuNoVs) have caused around 685 million cases of infection and nearly $60 billion in losses every year. Despite their highly contagious nature, an effective vaccine for HuNoVs has yet to become commercially available. Therefore, rapid detection and subtyping of noroviruses is crucial for preventing viral spread. Over the past half century, there has been monumental progress in the development of techniques for the detection and analysis of noroviruses. However, currently no rapid, portable assays are available to detect and subtype infectious HuNoVs. The purpose of this review is to survey and present different analytical techniques for the detection and characterization of noroviruses.

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Section: Other Optical Biosensor Methodsmentioning

confidence: 99%

Section: Other Optical Biosensor Methodsmentioning

confidence: 99%

Section: Other Optical Biosensor Methodsmentioning

confidence: 99%

Section: Other Optical Biosensor Methodsmentioning

confidence: 99%

See 2 more Smart Citations

A Survey of Analytical Techniques for Noroviruses

Liu

Moore

2020

Foods

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“…A molecular motor F 0 F 1 -ATPase biosensor is designed to detect foodborne noroviruses based on probe-RNA specific binding 123 . A specific probe is designed to encompass a conservative region of noroviruses; and F 0 F 1 -ATPase in chromatophore is constructed as a molecular motor to drive the biosensor in noroviruses capturing.…”

Section: Other Advanced Technologies In Poc Of Foodborne Virusesmentioning

confidence: 99%

Recent Advances in Biosensor Development for Foodborne Virus Detection

Neethirajan¹,

Ahmed²,

Chand³

et al. 2017

Nanotheranostics

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Outbreaks of foodborne diseases related to fresh produce have been increasing in North America and Europe. Viral foodborne pathogens are poorly understood, suffering from insufficient awareness and surveillance due to the limits on knowledge, availability, and costs of related technologies and devices. Current foodborne viruses are emphasized and newly emerging foodborne viruses are beginning to attract interest. To face current challenges regarding foodborne pathogens, a point-of-care (POC) concept has been introduced to food testing technology and device. POC device development involves technologies such as microfluidics, nanomaterials, biosensors and other advanced techniques. These advanced technologies, together with the challenges in developing foodborne virus detection assays and devices, are described and analysed in this critical review. Advanced technologies provide a path forward for foodborne virus detection, but more research and development will be needed to provide the level of manufacturing capacity required.

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Fluorometric determination of Vibrio parahaemolyticus using an F0F1-ATPase-based aptamer and labeled chromatophores

Duan

Zhang

et al. 2018

Microchim Acta

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An FF-ATPase-based aptasensor is described for the fluorometric determination of Vibrio parahaemolyticus. Chromatophores containing FF-ATPases were first prepared from Rhodospirillum rubrum cells. Then, an aptamer-functionalized chromatophore acts as the capture probe, and a chromatophore labeled with the pH probe fluorescein acts as the signalling probe. In the presence of V. parahaemolyticus, the rotation rate of FF-ATPase is decreased due to the formation of the aptamer-chromatophore complex. This leads to a retarded proton flux out of the chromatophores. As a result, the pH value inside the chromatophores is reduced, and the fluorescence of the pH probe F1300 is accordingly decreased. The relative fluorescence varies linearly over the 15 to 1.5 × 10 cfu·mL Vibrio parahaemolyticus concentration range, and the limit of detection is 15 cfu·mL. The method was applied to analyze artificially contaminated salmon samples where it showed excellent perfomance. Graphical abstract In this assay, aptamer functionalized chromatophores act as a capture probe, and the fluoresce in labeled chromatophores as signalling probe. The formation of aptamer-chromatophore complex leads to a retarded proton flux out of the chromatophores. As a result, the pH value inside the chromatophores is reduced, and fluorescence intensity is accordingly decreased.

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A rapidly new-typed detection of norovirus based on F0F1-ATPase molecular motor biosensor

Cited by 9 publications

References 34 publications

A Survey of Analytical Techniques for Noroviruses

A Survey of Analytical Techniques for Noroviruses

Recent Advances in Biosensor Development for Foodborne Virus Detection

Fluorometric determination of Vibrio parahaemolyticus using an F0F1-ATPase-based aptamer and labeled chromatophores

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