A rapid, simple and simultaneous detection of 2-keto-, 5-keto-and 2,5-diketogluconic acids by thin-layer chromatography in culture media of acetic acid bacteria

Gosselé, F.; Swings, Jean; Ley, J. De

doi:10.1016/s0172-5564(80)80039-x

Cited by 41 publications

(36 citation statements)

References 6 publications

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“…europaeus. Remarkably, the ubiquinone system of strains LTH 2460T and LTH is consistent with the systems of species within the corresponding clusters (Swings, 1992).…”

Section: Discussionsupporting

confidence: 68%

“…(i) Growth in the presence of 30 YO (w/v) glucose. This property is shared with strain LTH 2458T but is only described otherwise as a characteristic of the species Acetobacter diazotrophicus (Swings, 1992). (ii) Growth on ethanol in AE broth in the presence of 0-8 % (w/v) acetic acid.…”

Section: Discussionmentioning

confidence: 99%

“…Strains isolated from vinegar fermentations have been allotted to species of the genus Acetobacter, e.g. Acetobacter aceti, Acetobacter hansenii, Acetobacter pasteurianus and Acetobacter xylinus (Kittelmann et al, 1989;Swings, 1992). In addition, Sievers et al (1 992) described Acetobacter europaeus as a new species which represents the main flora component in industrial vinegar fermenters in Central Europe.…”

Section: Introductionmentioning

confidence: 99%

“…For determination of DNA similarities, 5 pg chromosomal DNA was denatured (0.05 x SSC and 0.4 M NaOH, 25 "C, 20 min) and transferred to a nylon membrane (Qiabrane; Qiagen) using a dot-blot apparatus (Schleicher & Schull). The DNA was immobilized at 80 "C for 1 h. The accessibility of DNA was determined by hybridization with the radiolabelled 16s rDNA bacterial probe EUB 338 (Amann et al, 1990) as described previously (Hertel et al, 1992). The probe-specific hybridization temperature and washing temperature were 45 and 55 "C, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…The radioactive dot-blot hybridization with the organismspecific oligonucleotide probe was performed as described by Hertel et al (1992) with the following modifications: chromosomal DNA and Qiabrane nylon membranes were used and labelling of the oligonucleotide probe was carried out with the Ready-To-Go T4 Polynucleotide Kinase kit and [Y-~~PIATP.…”

Section: Introductionmentioning

confidence: 99%

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Description of Acetobacter oboediens sp. nov. and Acetobacter pomorum sp. nov., two new species isolated from industrial vinegar fermentations

Sokollek

Hertel

Hammes

1998

International Journal of Systematic Bacteriology

129

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S t uttg a rt, GermanyTwo strains of Acetobacter sp., LTH 2460T and LTH 2458T, have been isolated from running red wine and cider vinegar fermentations, respectively. Taxonomic characteristics of the isolates were investigated. Comparative analysis of the 165 rRNA sequences revealed > 99% similarity between strain LTH 2460T and the type strains of the related species Acetobacter europaeus and Acetobacter xylinus and between strain LTH 2458T and Acetobacter pasteurianus. On the other hand, low levels of DNA relatedness (< 34%) were determined in DNA-DNA similarity studies. This relatedness below the species level was consistent with specific physiological characteristics permitting clear identification of these strains within established species of acetic acid bacteria. Based on these results, the names Acetobacter oboediens sp. nov. and Acetobacterpomorum sp. nov. are proposed for strains LTH 2460T and LTH 2458T, respectively. The phylogenetic positions of the new species are reflected by a 165 rRNA-based tree. Furthermore, a 165 rRNA-targeted oligonucleotide probe specific for A. oboediens was constructed.

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“…europaeus. Remarkably, the ubiquinone system of strains LTH 2460T and LTH is consistent with the systems of species within the corresponding clusters (Swings, 1992).…”

Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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