A rapid paper-based blood typing method from droplet wicking

Hertaeg, Michael J.; Tabor, Rico F.; McLiesh, Heather

doi:10.1039/d0an01896a

Cited by 15 publications

(16 citation statements)

References 33 publications

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“…A rapid paper-based blood group testing method from droplet wicking was found to have a high sensitivity, which was sensitive to antibody dilution between 1:2 and 1:8. It can determine ABO groups within 10 seconds and RH(D) for 20 seconds (29). The use of low-density and thick paper was more sensitive to detection (29).…”

Section: Novel Paper-based Testingmentioning

confidence: 99%

“…It can determine ABO groups within 10 seconds and RH(D) for 20 seconds (29). The use of low-density and thick paper was more sensitive to detection (29).…”

Section: Novel Paper-based Testingmentioning

confidence: 99%

“…Additionally, bioactive paper for blood group typing has been established by observing RBCs' haemagglutination (30)(31)(32)(33), such as Kleenex paper towels (34,35). The low cost, quickness, portability, and ease of paper-based testing holds considerable potential for point-of-care blood group typing with naked-eye readouts (27,29,36). The paper-based analytical device may be further developed for future applications in blood group testing.…”

Section: Novel Paper-based Testingmentioning

confidence: 99%

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Blood Group Testing

Guo

2022

Front. Med.

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Red blood cell (RBC) transfusion is one of the most frequently performed clinical procedures and therapies to improve tissue oxygen delivery in hospitalized patients worldwide. Generally, the cross-match is the mandatory test in place to meet the clinical needs of RBC transfusion by examining donor-recipient compatibility with antigens and antibodies of blood groups. Blood groups are usually an individual's combination of antigens on the surface of RBCs, typically of the ABO blood group system and the RH blood group system. Accurate and reliable blood group typing is critical before blood transfusion. Serological testing is the routine method for blood group typing based on hemagglutination reactions with RBC antigens against specific antibodies. Nevertheless, emerging technologies for blood group testing may be alternative and supplemental approaches when serological methods cannot determine blood groups. Moreover, some new technologies, such as the evolving applications of blood group genotyping, can precisely identify variant antigens for clinical significance. Therefore, this review mainly presents a clinical overview and perspective of emerging technologies in blood group testing based on the literature. Collectively, this may highlight the most promising strategies and promote blood group typing development to ensure blood transfusion safety.

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Section: Novel Paper-based Testingmentioning

confidence: 99%

“…It can determine ABO groups within 10 seconds and RH(D) for 20 seconds (29). The use of low-density and thick paper was more sensitive to detection (29).…”

Section: Novel Paper-based Testingmentioning

confidence: 99%

Section: Novel Paper-based Testingmentioning

confidence: 99%

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Blood Group Testing

Guo

2022

Front. Med.

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“…Paper-based colorimetric assays, featuring rapidity and convenience, hold great promise for point-of-care (POC) blood typing with naked-eye readouts. − However, these approaches frequently encounter inevitable environmental interference and low precision during the process of readout. , Moreover, the simultaneously grouping of multiplexed blood types such as ABO and Rhesus (Rh) always depends on high-throughput signal transduction strategies and highly cost-effective readout methods. − Our group previously invented a dye-assisted grouping methodology for rapid blood typing by identifying blood component-induced color changes after staining these components using the dye bromocresol green (BCG); we also explored a disk-based approach for multiplexed blood grouping within 2 min by using machine learning-empowered spectrometry . However, the latter still faces difficulties in dealing with field-deployable samples because spectrometers with an integrating sphere represent a heavy burden to guarantee the stability and repeatability of a result when measuring the resultant reflectance spectra, which significantly lowers the on-site feasibility.…”

Section: Introductionmentioning

confidence: 99%

Flipped Quick-Response Code Enables Reliable Blood Grouping

Zhang

Liu

et al. 2021

ACS Nano

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Accurate and rapid blood typing plays a vital role in a variety of biomedical and forensic scenarios, but recognizing weak agglutination remains challenging. Herein, we demonstrated a flipping identification with a prompt error-discrimination (FLIPPED) platform for automatic blood group readouts. Bromocresol green dye was exploited as a characteristic chromatography indicator for the differentiation of plasma from whole blood by presenting a teal color against a brown color. After integrating these color changes into a quick-response (QR) code, prompt typing of ABO and Rhesus groups was automatically achieved and data could be uploaded wirelessly within 30 s using a commercially available smartphone to facilitate blood cross-matching. We further designed a color correction model and algorithm to remove potential errors from scanning angles and ambient light intensities, by which weak agglutination could be accurately recognized. With comparable accuracy and repeatability to classical column assay in grouping 450 blood samples, the proposed approach further demonstrates to be a versatile sample-to-result platform for clinical diagnostics, food safety, and environmental monitoring.

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“…By means of blood agglutination with different channel lengths, users can determine whether they are sick or have a rare blood type. Furthermore, a paper-based device can also be used for fast blood typing, and the cost is relatively lower than the cost of other devices [30].…”

Section: Introductionmentioning

confidence: 99%

Micro-Droplet Platform for Exploring the Mechanism of Mixed Field Agglutination in B3 Subtype

Chen

et al. 2021

Biosensors

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B3 is the most common subtype of blood group B in the Taiwanese population, and most of the B3 individuals in the Taiwanese population have the IVS3 + 5 G > A (rs55852701) gene variation. Additionally, a typical mixed field agglutination is observed when the B3 subtype is tested with anti-B antibody or anti-AB antibody. The molecular biology of the gene variation in the B3 subtype has been identified, however, the mechanism of the mixed field agglutination caused by the type B3 blood samples is still unclear. Therefore, the purpose of this study was to understand the reason for the mixed field agglutination caused by B3. A micro-droplet platform was used to observe the agglutination of type B and type B3 blood samples in different blood sample concentrations, antibody concentrations, and at reaction times. We found that the agglutination reaction in every droplet slowed down with an increase in the dilution ratio of blood sample and antibody, whether type B blood or type B3 blood was used. However, as the reaction time increased, the complete agglutination in the droplet was seen in type B blood, while the mixed field agglutination still occurred in B3 within 1 min. In addition, the degree of agglutination was similar in each droplet, which showed high reproducibility. As a result, we inferred that there are two types of cells in the B3 subtype that simultaneously create a mixed field agglutination, rather than each red blood cell carrying a small amount of antigen, resulting in less agglutination.

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A rapid paper-based blood typing method from droplet wicking

Abstract: Paper-based diagnostics are leading the field of low-cost, point of care analytical techniques. However, large scale testing facilities such as hospitals are still primarily using the gel column agglutination technique....

Cited by 15 publications

References 33 publications

Blood Group Testing

Blood Group Testing

Flipped Quick-Response Code Enables Reliable Blood Grouping

Micro-Droplet Platform for Exploring the Mechanism of Mixed Field Agglutination in B3 Subtype

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