Micro-Droplet Platform for Exploring the Mechanism of Mixed Field Agglutination in B3 Subtype

Chen, Ding‐Ping; Chen, Chen; Wu, Pei‐Yu; Lin, Yi‐Ying; Lin, Wei-Tzu; Yan, Yi-Liang

doi:10.3390/bios11080276

Cited by 2 publications

(2 citation statements)

References 30 publications

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“…Not only the image observation as a quantitative result but also the naked-eye qualitative criteria requiring no prior experience could identify the blood agglutination on the chip. According to some studies, observing blood agglutination on a chip requires only quantitative results from skilled operators [23][24][25]. The naked-eye observation is an important interpretation because it is the first step to screening blood typing results.…”

Section: Image Observation Of Blood Agglutinationmentioning

confidence: 99%

Hemagglutination Detection with Paper–Plastic Hybrid Passive Microfluidic Chip

2021

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Hemagglutination is a critical reaction that occurs when antigens expressed on red blood cells (RBCs) react with the antibodies used for blood typing. Even though blood typing devices have been introduced to the market, they continue to face several limitations in terms of observation by the eye alone, blood manipulation difficulties, and the need for large-scale equipment, particularly process automated machines. Thus, this study aimed to design, fabricate, and test a novel hybrid passive microfluidic chip made of filter paper and polymer using a cost-effective xurography manufacturing technique. This chip is referred to as the microfluidic paper–plastic hybrid passive device (PPHD). A passive PPHD does not require external sources, such as a syringe pump. It is composed of a paper-based component that contains dried antibodies within its porous paper and a polymer component that serves as the detection zone. A single blood sample was injected into the chip’s inlet, and classification was determined using the mean intensity image. The results indicated that embedded antibodies were capable of causing RBC agglutination without a saline washing step and that the results could be classified as obviously agglutination or nonagglutination for blood typing using both the naked eye and a mean intensity image. As a proof-of-concept, this study demonstrated efficiency in quantitative hemagglutination measurement within a passive PPHD for blood typing, which could be used to simplify blood biomarker analysis.

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Section: Image Observation Of Blood Agglutinationmentioning

confidence: 99%

Hemagglutination Detection with Paper–Plastic Hybrid Passive Microfluidic Chip

2021

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“…Furthermore, a digital microfluidic device with droplet manipulation was used for blood agglutination assays [ 37 ]. Moreover, the current study group utilized a similar concept to study the mechanism of mixed-field agglutination in subtypes [ 38 ]. These studies showed the potential of the microdroplet platform for blood typing.…”

Section: Introductionmentioning

confidence: 99%

Irregular Antibody Screening Using a Microdroplet Platform

Chen,

Wu,

Lin

2023

Biosensors

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The screening procedure for antibodies is considered the most tedious among the three pretransfusion operations, i.e., ABO and Rhesus (Rh) typing, irregular antibody screening/identification, and crossmatching tests. The commonly used screening method for irregular antibodies in clinics at present is a manual polybrene test (MP). The MP test involves numerous reagent replacement and centrifuge procedures, and the sample volume is expected to be relatively less. Herein, screening red blood cells (RBCs) and serum irregular antibodies are encapsulated in microdroplets with a diameter of ~300 μm for a hemagglutination reaction. Owing to the advantage of spatial limitation in microdroplets, screening RBCs and irregular antibodies can be directly agglutinated, thereby eliminating the need for centrifugation and the addition of reagents to promote agglutination, as required by the MP method. Furthermore, the results for a large number of repeated tests can be concurrently obtained, further simplifying the steps of irregular antibody screening and increasing accuracy. Eight irregular antibodies are screened using the proposed platform, and the results are consistent with the MP method. Moreover, the volume of blood samples and antibodies can be reduced to 10 μL and 5 μL, respectively, which is ten times less than that using the MP method.

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Micro-Droplet Platform for Exploring the Mechanism of Mixed Field Agglutination in B3 Subtype

Cited by 2 publications

References 30 publications

Hemagglutination Detection with Paper–Plastic Hybrid Passive Microfluidic Chip

Hemagglutination Detection with Paper–Plastic Hybrid Passive Microfluidic Chip

Irregular Antibody Screening Using a Microdroplet Platform

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