A rapid molecular method for differentiating two special forms (lycopersici and radicis-lycopersici) of Fusarium oxysporum

Attitalla, Idress Hamad; Fatehi, Jamshid; Levenfors, Jens P.; Brishammar, Sture

doi:10.1017/s0953756204000322

Cited by 20 publications

(8 citation statements)

References 38 publications

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“…Particularly in VCG identification, while some formae speciales conform to only one VCG, others may belong to many VCGs. For this reason, although VCG identification may be beneficial, it cannot be utilized as a universal method in identifying formae speciales or isolates that are not pathogenic (Attitalla et al 2004). However, due to the greater accuracy and reliability of PCR for identification purposes, putting into practice the diagnosis of the disease as a result of this method will be quicker and easier.…”

Section: Discussionmentioning

confidence: 99%

PCR detection of Fusarium oxysporum f.sp. radicis-lycopersici and races of F. oxysporum f.sp. lycopersici of tomato in protected tomato growing areas of Eastern Mediterranean Region of Turkey

Çolak¹

2013

Turk J Agric For

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Levofloxacin was labeled with 99m Tc using cysteine • HCl as co-ligand and SnCl 2 .2H 2 O as reducing agent. The influence of various parameters such as amount of cysteine • HCl, reducing agent, pH value, and reaction time on labeling process was studied. After optimizing the conditions the labeling was performed at pH 5 using 1 mg of levofloxacin, 500 μ g of cysteine• HCl, 50 μ g of SnCl 2 .2H 2 O, and 15 min reaction time. The radiochemical purity was determined with the help of instant thin layer chromatography (ITLC) and reverse phase high performance liquid chromatography (RP-HPLC) which was more than 95% and was stable for up to 6 h. Biodistribution of 99m Tc-levofloxacin ( 99m Tc-lefx) was studied in infection induced rat models using live Staphylococcus aureus and heat killed S. aureus (sterile inflammation model). In the case of the live S. aureus induced abscess model, the accumulation of 99m Tc-lefx at target was 3.96, which was higher than that of 99m Tc-ciprofloxacin ( 99m Tc-cifx), taken as the control.

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Section: Discussionmentioning

confidence: 99%

PCR detection of Fusarium oxysporum f.sp. radicis-lycopersici and races of F. oxysporum f.sp. lycopersici of tomato in protected tomato growing areas of Eastern Mediterranean Region of Turkey

Çolak¹

2013

Turk J Agric For

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“…Elias et al 23 studied the population structure of Fol based on RFLP analysis of the genomic DNA which provided genetic evidence that Vegetative Compatibility Grouping (VCG) was an indicator of evolutionary origin. Based on Vegetative Compatibility, mtDNA RFLP, IGS and isozyme polymorphism, several researchers showed that isolates of Fol might represent two genetically distinct evolutionary lineages 24 25 26 27 . Hirano and Arie 28 , compared the endopolygalacturonase ( pg1) and exopolygalacturonase ( pgx4 ) gene sequences of Fol and Forl from Japan for their differentiation based on PCR.…”

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Molecular phylogeny, pathogenicity and toxigenicity of Fusarium oxysporum f. sp. lycopersici

Nirmaladevi

Mudili

Srivastava

et al. 2016

Sci Rep

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The present study aimed at the molecular characterization of pathogenic and non pathogenic F. oxysporum f. sp. lycopersici strains isolated from tomato. The causal agent isolated from symptomatic plants and soil samples was identified based on morphological and molecular analyses. Pathogenicity testing of 69 strains on five susceptible tomato varieties showed 45% of the strains were highly virulent and 30% were moderately virulent. Molecular analysis based on the fingerprints obtained through ISSR indicated the presence of wide genetic diversity among the strains. Phylogenetic analysis based on ITS sequences showed the presence of at least four evolutionary lineages of the pathogen. The clustering of F. oxysporum with non pathogenic isolates and with the members of other formae speciales indicated polyphyletic origin of F. oxysporum f. sp. lycopersici. Further analysis revealed intraspecies variability and nucleotide insertions or deletions in the ITS region among the strains in the study and the observed variations were found to be clade specific. The high genetic diversity in the pathogen population demands for development of effective resistance breeding programs in tomato. Among the pathogenic strains tested, toxigenic strains harbored the Fum1 gene clearly indicating that the strains infecting tomato crops have the potential to produce Fumonisin.

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“…radicislycopersici (FORL) olmak üzere iki ayrı formu bulunmaktadır. FOL Fusarium solgunluğuna, FORL ise Fusarium kök ve kök boğazı çürümelerine neden olmaktadır (Attitalla et al 2004).…”

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Effects of Potassium, Salicylic Acid and Humic Acid Applications on Seedling Emergence and Fusarium Wilt (Fusarium oxysporum f.sp. lycopersici) in Tomato

Gülser¹,

Tüfenkçi²,

Demir³

2014

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

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Özet: Bu çalışmada potasyum, salisilik asit, ve humik asit uygulamalarının domateste fide çıkışı ve Fusarium (Fusarium oxysporum f. sp. lycopersici) solgunluğuna etkileri kontrollü koşullarda araştırılmıştır. Denemede patojen ile bulaşık saksı topraklarına potasyum (K) (0 ve 200 ppm), salisilik asit (SA) (0 ve 0.1 mmol) ve humik asit (HA)'in (0-200 ppm) farklı iki dozu uygulanmıştır. Deneme sonunda fusarium kontrol (F) uygulamasına göre en düşük hastalık şiddeti sırasıyla SA (%.40.3) ve HA uygulamalarında (%40.7) elde edilmiştir. Çimlenme oranları en yüksek HA, steril kontrol (C) ve SA uygulamalarında sırasıyla %80, %77.7 ve %71 olarak belirlenmiştir. Çimlenme hızı ise en yüksek SA, Potasyum-Salisilik Asit (KSA) ve F uygulamalarında sırasıyla 11.4, 8.17 ve 6.63 gün olarak bulunmuştur. Genel olarak SA ve HA uygulamalarının F. oxysporum f. sp. lycopersici hastalık şiddetini azalttığı, domateste fide çıkışı ve gelişimine olumlu etkileri olduğu belirlenmiştir.

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A rapid molecular method for differentiating two special forms (lycopersici and radicis-lycopersici) of Fusarium oxysporum

Cited by 20 publications

References 38 publications

PCR detection of Fusarium oxysporum f.sp. radicis-lycopersici and races of F. oxysporum f.sp. lycopersici of tomato in protected tomato growing areas of Eastern Mediterranean Region of Turkey

PCR detection of Fusarium oxysporum f.sp. radicis-lycopersici and races of F. oxysporum f.sp. lycopersici of tomato in protected tomato growing areas of Eastern Mediterranean Region of Turkey

Molecular phylogeny, pathogenicity and toxigenicity of Fusarium oxysporum f. sp. lycopersici

Effects of Potassium, Salicylic Acid and Humic Acid Applications on Seedling Emergence and Fusarium Wilt (Fusarium oxysporum f.sp. lycopersici) in Tomato

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