A rapid HPLC method for determination of Sudan dyes and Para Red in red chilli pepper

Ertaş, Erdal; Özer, Hayrettin; Alasalvar, Cesarettin

doi:10.1016/j.foodchem.2007.01.010

Cited by 176 publications

(90 citation statements)

References 8 publications

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“…In the last 5 years several analytical methods based on GC [10], LC [11,12], or CE [13] have been reported in the literature. However, except when very expensive HPLC-MS/MS instrumentation is used [14,15], the direct detection of the target analytes in complex food matrices is a difficult task, and some sample clean-up treatment is mandatory before performing the analysis.…”

Section: Introductionmentioning

confidence: 99%

Determination of banned Sudan dyes in food samples by molecularly imprinted solid phase extraction‐high performance liquid chromatography

Baggiani¹,

Anfossi

Baravalle

et al. 2009

J of Separation Science

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Original PaperDetermination of banned Sudan dyes in food samples by molecularly imprinted solid phase extraction-high performance liquid chromatography A method for molecularly imprinted SPE of banned Sudan azo-dyes from food samples was investigated. The molecularly imprinted polymer was obtained by suspension polymerization using 1-(4-chlorophenyl)azonaphthalen-2-ol as the mimic template. The molecular recognition properties of imprinted beads were evaluated for use as a SPE sorbent, in order to develop a selective extraction protocol for the Sudan class of dyes. The optimized extraction protocol resulted in a reliable molecularly imprinted SPE (MISPE) method suitable for HPLC analysis. It was selective for the main analyte, Sudan I, and the related azo-dyes Sudan II, III, IV, Sudan Red B, and Sudan Red 7B, while the permitted azo-dyes Allura Red AC, Neococcin, and Sunset Yellow FCF were not extracted. The method was tested for Sudan I, II, III, and IV in five different food samples (hot chilli pepper, hot chilli tomato sauce, sausage, tomato sauce, and hard boiled egg yolk) at three concentration levels (15, 100, and 300 lg/g). It demonstrated itself to be insensitive to the presence of different complex matrices, precise, accurate, and with good recovery rates (85 -101%). The LOD and LOQ were satisfactory for most analytical determinations.

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Section: Introductionmentioning

confidence: 99%

Determination of banned Sudan dyes in food samples by molecularly imprinted solid phase extraction‐high performance liquid chromatography

Baggiani¹,

Anfossi

Baravalle

et al. 2009

J of Separation Science

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“…A rapid HPLC method with UV-Vis detector for the determination of sudan and para red dyes in red chilli peppers on a reverse phase C-18 column with isocratic elution, using a mobile phase of acetonitrile/methanol with detection at 506 nm was notified (Ertas et al, 2007). In the same year a reversed phase HPLC coupled with mass spectrometry was appeared in references for the estimation of these dyes in acetonitrile extract acidified with acetic acid (Botek et al, 2007).…”

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confidence: 99%

Estimation of Para Red Dye in Chilli Powder and Tomato Sauces by a Simple Spectrophotmetric Method followed by Thin layer Chromatography

Mustafa¹,

Khan²,

Sultana³

et al. 2013

Journal of Applied Sciences and Environmental Management

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A number of grinded chilli powder samples and tomato sauce samples were collected from various localities of Karachi city. All the samples were estimated for the presence of para red which is an azo dye and more specifically belongs from the group of sudan dyes. According to the regulation of various governmental agencies its use in food is strictly prohibited because of its ability to form carcinogenic compounds. Although there were a number of sophisticated chromatographic methods for the estimation of this dye but we developed here a simple, convenient, expeditious spectrophotometric method followed by thin layer chromatography technique and compared the results by single point and multiple point external standard methods. On the basis of these results we also specify the localities of Karachi city where the contamination is more prominent. © JASEM

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“…There are other methods for the quantitative detection and characterization of azo dyes, including electronic spectroscopy [11], infrared spectroscopy [12][13][14], mass spectroscopy [15,16], gas chromatography/mass spectroscopy, high-performance liquid chromatography (HPLC) with UV-Vis and mass detectors [17][18][19][20][21][22][23]. It is well known that electrochemical methods are the most widely used in direct determination of chemical molecules, as they are not only rapid and sensitive, but also more feasible for microanalysis.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Behavior of Acid Orange 7 by Cyclic Voltammetry in Different Solvents

Ennouri¹,

Panizza²,

Mhiri³

et al. 2017

Port. Electrochim. Acta

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In the present work, the electrochemical oxidation of acid orange 7 (AO7) on a glassy carbon (GC) electrode has been investigated by cyclic voltammetry in different solvents: aqueous solvent (H2O), dimethyl sulfoxide (DMSO) and acetone (ACE), using sulfuric acid as a supporting electrolyte. The analysis of AO7 oxidation voltammograms in different solvents showed that the more donor numbers, the easier it becomes the oxidation of AO7. The experimental parameters show that the oxidation peak current of AO7 was linearly proportional to its concentration in a range from 0.04 mM to 0.2 mM. The limit of detection was estimated by gradually decreasing the concentration levels of AO7, and was found to be 6.6 µM.

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A rapid HPLC method for determination of Sudan dyes and Para Red in red chilli pepper

Cited by 176 publications

References 8 publications

Determination of banned Sudan dyes in food samples by molecularly imprinted solid phase extraction‐high performance liquid chromatography

Determination of banned Sudan dyes in food samples by molecularly imprinted solid phase extraction‐high performance liquid chromatography

Estimation of Para Red Dye in Chilli Powder and Tomato Sauces by a Simple Spectrophotmetric Method followed by Thin layer Chromatography

Electrochemical Behavior of Acid Orange 7 by Cyclic Voltammetry in Different Solvents

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