A rapid chromatographic strip test for the pen-side diagnosis of rinderpest virus

Brüning, A; Bellamy, Kathryn; Talbot, Duncan; Anderson, J.

doi:10.1016/s0166-0934(99)00068-3

Cited by 42 publications

(14 citation statements)

References 6 publications

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“…The technique permits rapid diagnosis, allowing time for the early implementation of control measures to reduce the possibility of spread of FMD. The LFA has been developed widely to support clinical diagnosis of different diseases [225][226][227], including FMD [170,228]. A rapid lateral-flow assay (LFA) based on FMDV antigen detection, which is easy to use and can be utilized on the farm to reduce the time required for transport and laboratory diagnosis.…”

Section: Pen Side Diagnostic Approach Routine Diagnosis Ofmentioning

confidence: 99%

A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools

Longjam

Deb

Sarmah

et al. 2011

Veterinary Medicine International

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Foot-and-mouth disease (FMD) is one of the highly contagious diseases of domestic animals. Effective control of this disease needs sensitive, specific, and quick diagnostic tools at each tier of control strategy. In this paper we have outlined various diagnostic approaches from old to new generation in a nutshell. Presently FMD diagnosis is being carried out using techniques such as Virus Isolation (VI), Sandwich-ELISA (S-ELISA), Liquid-Phase Blocking ELISA (LPBE), Multiplex-PCR (m-PCR), and indirect ELISA (DIVA), and real time-PCR can be used for detection of antibody against nonstructural proteins. Nucleotide sequencing for serotyping, microarray as well as recombinant antigen-based detection, biosensor, phage display, and nucleic-acid-based diagnostic are on the way for rapid and specific detection of FMDV. Various pen side tests, namely, lateral flow, RT-LAMP, Immunostrip tests, and so forth. are also developed for detection of the virus in field condition.

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Section: Pen Side Diagnostic Approach Routine Diagnosis Ofmentioning

confidence: 99%

A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools

Longjam

Deb

Sarmah

et al. 2011

Veterinary Medicine International

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Section: Discussionmentioning

confidence: 99%

Simple and Rapid Lateral-Flow Assay for the Detection of Foot-and-Mouth Disease Virus

Oem

Ferris

Lee

et al. 2009

Clin Vaccine Immunol

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A simple lateral-flow assay (LFA) based on a monoclonal antibody (MAb 70-17) was developed for the detection of foot-and-mouth disease virus (FMDV) under nonlaboratory conditions. The LFA was evaluated with epithelial suspensions (n ‫؍‬ 704) prepared from current and historical field samples which had been submitted to the Pirbright Laboratory (United Kingdom) and from negative samples (n ‫؍‬ 100) collected from naïve animals in Korea. Four FMDV serotypes (type O, A, Asia 1, and C) were detected in the LFA, but not the remaining three FMDV serotypes (SAT 1, SAT 2, and SAT 3). The diagnostic sensitivity of the LFA for FMDV types O, A, C, and Asia 1 was similar, at approximately 87.3%, to that of 87.7% obtained with antigen enzyme-linked immunosorbent assay (Ag-ELISA). The diagnostic specificity of the LFA was 98.8%, compared to 100% for the Ag-ELISA. These results demonstrate that the LFA using the FMDV MAb 70-17 to detect FMDV is a supportive method for taking rapid measurements at the site of a suspected foot-and-mouth disease outbreak in Asia before diagnosing the disease in the laboratory, thereby offering the possibility of implementing control procedures more rapidly.The foot-and-mouth disease virus (FMDV), an Aphthovirus of the Picornaviridae family, causes a highly contagious and economically important disease in cloven-hoofed animals. Seven serotypes and many subserotypes have been identified. Footand-mouth disease (FMD) has been recognized as the most important constraint to international trade in animals and animal products (11,12).Typical cases of FMD are characterized by the formation of vesicles and epithelial erosions of the snout, tongue, hard and soft palate, coronary band, and feet. FMD cannot be distinguished clinically from other vesicular diseases, such as swine vesicular disease (SVD) and vesicular stomatitis (VS). Consequently, laboratory-based tests (virus isolation or demonstration of FMDV antigen or nucleic acid) are required for differential diagnosis. At present, routine diagnosis of FMD is made at several laboratories by the combined use of enzyme-linked immunosorbent assay (ELISA) and virus isolation techniques, supplemented by reverse transcriptase PCR (RT-PCR) (13-15, 18). However, most of these diagnostic methods require the availability of a dedicated laboratory facility, highly trained laboratory personnel, stable reagents, and multistep sample handling or preparation. In particular, virus isolation requires a laboratory cell culture facility, which can be difficult and expensive to maintain, besides requiring 4 to 6 days for test completion. Management of the logistical considerations associated with sample collection and transport is also required.A rapid and easy-to-perform test, which would allow for on-site diagnosis to be made in the case of a suspected disease outbreak, would circumvent problems associated with the transportation of samples to the laboratory and would be especially useful for a faster diagnosis in areas where the disease is endemic. Since FMD is extremely...

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“…The membrane strips were assembled into a device as described previously (Brüning et al 1999;Ferris et al 2009). 3 and 4).…”

Section: Adsorption Of Gold/rtasp Conjugate To Filtersmentioning

confidence: 99%

Development and laboratory evaluation of a lateral flow device (LFD) for the serodiagnosis of Theileria annulata infection

Abdo

Kristersson²,

Seitzer

et al. 2010

Parasitol Res

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Several DNA-based and serological tests have been established for the detection of Theileria annulata infection, including polymerase chain reaction, reverse line blot and loop-mediated isothermal amplification, indirect enzyme-linked immunosorbent assay (ELISA), and competitive ELISA. In this study, we have applied knowledge from the development and application of a recombinant protein-based indirect ELISA and competitive ELISA to establish a rapid test for point-of-care diagnosis of T. annulata infection in the field to be used by the veterinarian. For the development of a lateral flow test, the recombinantly expressed T. annulata surface protein (TaSP) was applied as the test antigen and anti-TaSP antiserum as the control line. TaSP antigen conjugated to colloidal gold particles was used as the detection system for visualization at the test line for the binding of anti-TaSP antibody present in the serum of infected animals. The developed test specifically detected antibodies in the serum of animals experimentally infected with T. annulata and showed no cross-reactivity with serum from animals infected with other tested bovine pathogens (Trypanosoma brucei, Anaplasma marginale, Babesia bigemina, Babesia bovis, and Theileria parva). Testing of field samples was compared to results obtained by other serological tests, resulting in a sensitivity and specificity of 96.3% and 87.5% compared to indirect fluorescence antibody test, 98.7% and 81.8% compared to indirect ELISA, and 100% and 47.6% compared to competitive ELISA. In conclusion, a rapid test for the detection of T. annulata infection (T. annulata lateral flow device, Ta-LFD) has been developed, which is easy to perform, delivers results to be read by the naked eye within 10 min, and is suitable for the detection of infection in field samples.

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A rapid chromatographic strip test for the pen-side diagnosis of rinderpest virus

Cited by 42 publications

References 6 publications

A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools

A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools

Simple and Rapid Lateral-Flow Assay for the Detection of Foot-and-Mouth Disease Virus

Development and laboratory evaluation of a lateral flow device (LFD) for the serodiagnosis of Theileria annulata infection

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