A Rapid and Miniaturized Method for the Selection of Microbial Phenol Degraders Using Colourimetric Microtitration

Abstract

A high-throughput method is described, consisting of a colourimetric microtitration for screening phenol-degrading microorganisms, using a mixture of 4-aminoantipyrine and potassium ferricyanide as the colour indicator. This contemporary study summarizes a new method to determine phenol-degrading bacteria isolated from different areas. The method was used for testing a total of 72 bacteria collected from the natural environment and five known strains obtained from diagnostic and research laboratories employing 200 mg/L phenol (the linear range saturation concentration). Depending on the change in colour indicator, the degradation profiles of 11 strains of bacteria are shown, of which seven strains were able to degrade more than 80 % of phenol within 6-8 h, while the other four strains took 12-24 h. Two of the environmentally isolated strains showed high efficiency of phenol degradation and were confirmed by the high-performance liquid chromatography analysis. These strains were identified by 16S rRNA sequencing as unique (Escherichia coli moh1 and Bacillus cereus moh2) and were deposited in the GenBank of NCBI. Two pathogenic strains (Uropathogenic E. coli and Salmonella sp.) were found to be the fast degraders of phenol, which is of medical concern, as phenol is generally used as a disinfectant in hospitals. This method can be used for the estimation and screening of phenol degraders in a single step, for its application in bioremediation as well as in hospitals for screening the phenol resistance of pathogens.

Biodegradation of toxic organic compounds using a newly isolated Bacillus sp. CYR2

“…I-103 AB531392.1 (99% sequence identity), Bacillus thuringiensis KF150502.1 (100% sequence identity), and Bacillus cereus EU741099.1 (100% sequence identity) (Figure 1). Many authors reported about efficiency of Bacillus group for degradation of various toxic compounds [21][22][23][24].…”

An Integrated Suggestion to Decolourize Allura Red AC Dye in the Wastewater of Pharmaceutical Industries Using a Local Bacterial Isolates

“…For molecular identification of decolorized isolates, Two universal primers supplied by Macrogen Company as a lyophilized form were used for isolation of specific 16S rRNA gene. 18 Lyophilized primers (27F with sequence 5'AGAGTTTGATCCTGGCTCAG-3'and 1492R with sequence 5'TACGGTTACCTTGTTACGACTT-3'sequences), both were dissolved in a nuclease free water to give a final concentration of 100 pmol/μL as a stock solution. A working solution of these primers was prepared by adding 10 μL of primer stock solution…”

Poly-3-hydroxybutyrate (PHB) production from alkylphenols, mono and poly-aromatic hydrocarbons using Bacillus sp. CYR1: A new strategy for wealth from waste

“…Toledo et al (2006) reported that strains Bacillus pumilus, and Bacillus subtilis were able to grow and utilize PAH compounds such as naphthalene, phenanthrene, fluoranthene and pyrene. Kuang et al (2013) reported 62% degradation of phenol at 200 mg/l concentration using Bacillus fusiformis within 7 h. Fayidh et al (2015) reported 43% and 94% degradation of phenol at 200 mg/l concentration using Bacillus sp. and Bacillus cereus, respectively.…”