An Integrated Suggestion to Decolourize Allura Red AC Dye in the Wastewater of Pharmaceutical Industries Using a Local Bacterial Isolates

Abstract

Many types of dyes used in pharmaceutical and food industries such as (Allura Red AC) might be thrown out into the environment, leading to contaminating the natural water sources and its concentration in some tanks. To support the biological treatment, designed this project was to isolate and identify the bacteria from the biological treatment tanks, able to decolourize some industrial dyes waste used in the pharmaceutical industry in Iraq. Streaking on Tryptone soy agar with Allura red dye 0.001-mg/L, more than 11 single bacterial colonies were separated and tested for their ability on decolorization. In this paper, only Two isolates will be in focus for decolourizing. The Absorbance of Allura red at wavelength 510 nm of spectrometer demonstrated its clearly decreased within the incubation time 6–24 hours and depended on the initial concentration of the dye, which was done in MSM media. The isolates were identified by the VITEK-2 XL compound system as well as the 16S rRNA gene. The identification results showed that these isolates were related to Escherichia coli and Klebsiella pneumoniae.