Poly-3-hydroxybutyrate (PHB) production from alkylphenols, mono and poly-aromatic hydrocarbons using Bacillus sp. CYR1: A new strategy for wealth from waste

Reddy, M. Venkateswar; Mawatari, Yasuteru; Yajima, Yoshiaki; Seki, Chigusa; Hashizume, Tamotsu; Chang, Young-Cheol

doi:10.1016/j.biortech.2015.06.043

Cited by 57 publications

(16 citation statements)

References 29 publications

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“…and Pseudomonas sp., through the fermentation of various substrates [ 62 ]. Among the pure cultures, the bacteria Bacillus strains have been widely studied because of their potential in producing significant amounts of PHB from organic carbon substrates, such as glycerol, dairy wastes, agro-industrial wastes, food industry waste, fatty acids, toxic chemical compounds, and sugars [ 11 , 63 , 64 , 65 , 66 , 67 , 68 ].…”

Section: Resultsmentioning

confidence: 99%

Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1

et al. 2021

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Cheese whey (CW) can be an excellent carbon source for polyhydroxyalkanoates (PHA)-producing bacteria. Most studies have used CW, which contains high amounts of lactose, however, there are no reports using raw CW, which has a relatively low amount of lactose. Therefore, in the present study, PHA production was evaluated in a two-stage process using the CW that contains low amounts of lactose. In first stage, the carbon source existing in CW was converted into acetic acid using the bacteria, Acetobacter pasteurianus C1, which was isolated from food waste. In the second stage, acetic acid produced in the first stage was converted into PHA using the bacteria, Bacillus sp. CYR-1. Under the condition of without the pretreatment of CW, acetic acid produced from CW was diluted at different folds and used for the production of PHA. Strain CYR-1 incubated with 10-fold diluted CW containing 5.7 g/L of acetic acid showed the higher PHA production (240.6 mg/L), whereas strain CYR-1 incubated with four-fold diluted CW containing 12.3 g/L of acetic acid showed 126 mg/L of PHA. After removing the excess protein present in CW, PHA production was further enhanced by 3.26 times (411 mg/L) at a four-fold dilution containing 11.3 g/L of acetic acid. Based on Fourier transform infrared spectroscopy (FT-IR), and 1H and 13C nuclear magnetic resonance (NMR) analyses, it was confirmed that the PHA produced from the two-stage process is poly-β-hydroxybutyrate (PHB). All bands appearing in the FT-IR spectrum and the chemical shifts of NMR nearly matched with those of standard PHB. Based on these studies, we concluded that a two-stage process using Acetobacter pasteurianus C1 and Bacillus sp. CYR-1 would be applicable for the production of PHB using CW containing a low amount of lactose.

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Section: Resultsmentioning

confidence: 99%

Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1

et al. 2021

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“…In addition, Azotobacter SSB81 was able to utilize 1000 mg l -1 concentration of 2,4-D as sole carbon source and the growth of SSB81 was primarily inhibited by the presence of 2,4-D in the medium, but after 48 h growth rate was increased slowly [ 28 ]. In our study, strain CY-1 grew in 2,4-D in concentrations up to 700 mg l -1 , beyond this concentration (at 800 mg l -1 ) bacteria were unable to show growth up to 120 h. Bacteria belongs to Cupriavidus genus can produce polyhydroxybutyrate (PHB) [ 29 , 30 ], in addition to degradation of toxic compounds like 2,4-D. PHB is a type of biopolymer produced by many species of bacteria [ 31 , 32 ]. De Morais et al, [ 29 ] investigated the nanofibers production using PHB extracted from Spirulina and the bacteria Cupriavidus necator .…”

Section: Resultsmentioning

confidence: 99%

Bio-Augmentation of Cupriavidus sp. CY-1 into 2,4-D Contaminated Soil: Microbial Community Analysis by Culture Dependent and Independent Techniques

et al. 2015

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In the present study, a 2,4-dichlorophenoxyacetic acid (2,4-D) degrading bacterial strain CY-1 was isolated from the forest soil. Based on physiological, biochemical and 16S rRNA gene sequence analysis it was identified as Cupriavidus sp. CY-1. Further 2,4-D degradation experiments at different concentrations (200 to 800 mg l-1) were carried out using CY-1. Effect of NaCl and KNO3 on 2,4-D degradation was also evaluated. Degradation of 2,4-D and the metabolites produced during degradation process were analyzed using high pressure liquid chromatography (HPLC) and GC-MS respectively. The amount of chloride ions produced during the 2,4-D degradation were analyzed by Ion chromatography (IC) and it is stoichiometric with 2,4-D dechlorination. Furthermore two different types of soils collected from two different sources were used for 2,4-D degradation studies. The isolated strain CY-1 was bio-augmented into 2,4-D contaminated soils to analyze its degradation ability. Culture independent methods like denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP), and culture dependent methods like colony forming units (CFU) and most probable number (MPN) were used to analyze the survivability of strain CY-1 in contaminated soil. Results of T-RFLP were coincident with the DGGE analysis. From the DGGE, T-RFLP, MPN and HPLC results it was concluded that strain CY-1 effectively degraded 2,4-D without disturbing the ecosystem of soil indigenous microorganisms.

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“…17 PHB content of the native and recombinant strains was then determined according to Law and Slepecky procedure. 18,19 In this case, PHB in reaction with concentrated sulfuric acid in the vicinity of 100˚C water bath, was converted to crotonic acid through dehydration. 20 Absorbance of the resultant molecule was measured at 235 235 nm wavelength in spectrophotometer (Cecil BioAquarius, Cambridge, United Kingdom) against sulfuric acid blank, and quantified pursuant to the PHB standard curve.…”

Section: Cultivation and Phb Quantificationmentioning

confidence: 99%

Considerable increase in Poly(3-hydroxybutyrate) production via phbCgene overexpression in Ralstonia eutropha PTCC 1615

Barati
¹
,

Asgarani
²
,

Gharavi
³

et al. 2020

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IntroductionToday, there is a wide range of applications for synthetic plastics. However due to their resistance against natural destruction, nonrenewable sources, and toxic substances generated during their long run degradation in nature, they have become a great environmental problem. 1 Much attention has been paid to bioplastics which have sustainable sources and environmentally friendly elimination. 2 Bioplastics are polymers which are produced as storage materials by different organisms. An important group of biopolymers is called polyhydroxyalkanoate (PHA) that accumulates in some microorganisms as an energy reservoir. High molecular weight of the natural polyesters in range of 50-1000 kDa has led to properties like chemically synthesized plastics and so resulted in many uses. 3 Poly(3-hydroxybutyrate) (PHB) is a well-known member of PHA family. Ralstonia eutropha (also Alcaligenes eutrophus or Cupriavidus necator) is a model organism for PHB production. 4 Three enzymes conduct the polymer biosynthesis pathway in this bacterium. First step of the pathway is catalyzed by β-ketothiolase which combines two acetyl-CoA molecules to form acetoacetyl-CoA. Reduction of acetoacetyl-CoA to (R)-3-hydroxybutyryl-CoA is the next step which is carried out by acetoacetyl-CoA reductase. Finally, polymerization of (R)-3-hydroxybutyryl-CoA monomers as the last step occurs in the presence of PHB synthase. These enzymes are encoded by phbA, phbB and phbC genes, respectively. 5,6 Some PHAs such as PHB are produced commercially, but because of high production costs, they cannot yet compete with synthetic plastics. To decrease expenses,

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Poly-3-hydroxybutyrate (PHB) production from alkylphenols, mono and poly-aromatic hydrocarbons using Bacillus sp. CYR1: A new strategy for wealth from waste

Cited by 57 publications

References 29 publications

Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1

Two-Stage Polyhydroxyalkanoates (PHA) Production from Cheese Whey Using Acetobacter pasteurianus C1 and Bacillus sp. CYR1

Bio-Augmentation of Cupriavidus sp. CY-1 into 2,4-D Contaminated Soil: Microbial Community Analysis by Culture Dependent and Independent Techniques

Considerable increase in Poly(3-hydroxybutyrate) production via phbCgene overexpression in Ralstonia eutropha PTCC 1615

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