A Rapid and Label‐free SERS Detection Method for Biomarkers in Clinical Biofluids

Kong, Kien Voon; Leong, Weng Kee; Lam, Zhiyong; Gong, Tianxun; Goh, Douglas; Lau, Weber Kam On; Olivo, Malini

doi:10.1002/smll.201401713

Cited by 31 publications

(14 citation statements)

References 54 publications

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“…SERS is a powerful spectroscopic technique enabling for a highly sensitive detection due to the significant amplification of Raman signal from molecules attached to the metallic surface with nanometer size [ 110 , 111 ]. It has been widely used in various bio- or chemical sensing applications for probing of single molecules [ 111 – 113 ], molecular analysis [ 114 ], bio markers [ 115 – 119 ], and environmental monitoring [ 120 , 121 ]. It has even been used in forensic science for detection of explosives, drugs, blood, DNA, and fingerprints [ 122 ], since the Raman spectra of pyridine on silver electrode were first observed in 1974 [ 123 ] and their mechanism was analyzed in 1977 [ 124 ].…”

Section: M13 Phage-based Sers Applicationsmentioning

confidence: 99%

Recent advances in M13 bacteriophage-based optical sensing applications

Kim

Moon

2016

Nano Convergence

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Recently, M13 bacteriophage has started to be widely used as a functional nanomaterial for various electrical, chemical, or optical applications, such as battery components, photovoltaic cells, sensors, and optics. In addition, the use of M13 bacteriophage has expanded into novel research, such as exciton transporting. In these applications, the versatility of M13 phage is a result of its nontoxic, self-assembling, and specific binding properties. For these reasons, M13 phage is the most powerful candidate as a receptor for transducing chemical or optical phenomena of various analytes into electrical or optical signal. In this review, we will overview the recent progress in optical sensing applications of M13 phage. The structural and functional characters of M13 phage will be described and the recent results in optical sensing application using fluorescence, surface plasmon resonance, Förster resonance energy transfer, and surface enhanced Raman scattering will be outlined.

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Section: M13 Phage-based Sers Applicationsmentioning

confidence: 99%

Recent advances in M13 bacteriophage-based optical sensing applications

Kim

Moon

2016

Nano Convergence

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“…53 This sensor was tested with clinical urine samples and was able to differentiate between samples with pH 5.2 and 5.8 accurately. 124 Label-free detection of alpha-1 antitrypsin (A1AT), a potential biomarker for bladder cancer, 125 was also demonstrated with a functional chip prepared by first depositing the triosmium carbonyl cluster Os3(CO)10(μ-H))(μ-SCH2CH2COOH) onto BMFON, followed by the attachment of A1AT antibodies onto the carboxylic acid group (figure 19). Binding of A1AT with the antibodies induced changes in the CO vibrations which were concentration dependent but this had to be analyzed through principal component analysis (PCA).…”

Section: Sers-based Detection On Planar Substratementioning

confidence: 99%

“…Spectral changes of the CO vibrational frequencies, induced by the addition of protein, can be detected and quantified by SERS. Adapted from ref 125. with permission from John Wiley and Sons.particularly important for subcellular work (although PTIR, which uses the AFM-IR setup, is able to achieve a high resolution of 20-50 nm with IR imaging (see section 2.1.4)).…”

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confidence: 99%

Vibrational spectroscopy of metal carbonyls for bio-imaging and -sensing

Lam

Kong

Olivo

et al. 2016

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Transition metal carbonyls exhibit strong CO absorptions in the 2200-1800 cm(-1) region, which is free of interference from other functional groups. This feature has led to their applications in bio-imaging and -sensing, in particular through mid-IR, Raman and more recently, surface-enhanced Raman spectroscopy (SERS). Their use in mid-IR quantitative sensing based on vibrational intensities, and chemical sensing based on frequency shifts and vibrational lifetimes, is reviewed. Their development for Raman sensing following the breakthrough in SERS highlights the potential of coupling metal carbonyls to plasmonic nanostructures as novel optical materials for SERS-based bio-imaging and -sensing.

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“…In 2012, an estimated 8.2 million people died from cancer, and about 14 million new cases of cancer were diagnosed. 6 For example, loss of E-cadherin expression and increase of N-cadherin expression can cause a transformation of a normal epithelial cell to a cancerous one. Assaying cancer cells is a very effective approach towards early diagnosis of cancers since the occurrence and development of cancer are closely related to the change of cells, such as cell surface components, cell proliferation, and cell differentiation.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of magnetic Fe₃O₄–Au hybrids for sensitive SERS detection of cancer cells at low abundance

Qiu

Deng

et al. 2015

J. Mater. Chem. B

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This work reports a facile synthesis of magnetic hybrids (Fe 3 O 4 -Au hybrids) with high SERS activity for detecting a low abundance of cancer cells. We labeled Raman reporter molecule 4-aminothiophenol (4-ATP) and the antibody of CEA (anti-CEA) on Fe 3 O 4 -Au hybrid nanoparticles (anti-CEA/4-ATP/Fe 3 O 4 -Au) as SERS tags and anti-CEA-labeled Au NPs (anti-CEA/Au) as SERS-active substrates to improve detection sensitivity. In the presence of CEA positive-expressed cancer cells (such as non-small lung cancer cells, A549), sandwich structures were formed as both SERS tags and SERS-active substrates could be bound to CEA at cell surfaces, leading to the formation of SERS hot spots at the junction of SERS tags and SERSactive substrates. As a result, an enhanced SERS signal of 4-ATP arose, which could be used to detect CEA on cell surfaces. This assay can specifically detect CEA-expressed A549 cells at a very low abundance (B10 cells mL À1 ) and can be used for evaluating CEA expression levels of different cancer cells line. This assay has the advantages of high sensitivity (because the SERS technique itself enables the detection of very low concentration analytes, even single molecules), high specificity (because of the enhanced SERS signal arising in sandwich configuration, which is formed in specific antigen-antibody interaction events), and high efficiency (because the magnetic SERS tags can concentrate the captured cells and separate them from the complex detection system without repetitive washing steps). These features make our developed assay a rapid and facile method to sensitively detect a low abundance of cancer cells.

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A Rapid and Label‐free SERS Detection Method for Biomarkers in Clinical Biofluids

Cited by 31 publications

References 54 publications

Recent advances in M13 bacteriophage-based optical sensing applications

Recent advances in M13 bacteriophage-based optical sensing applications

Vibrational spectroscopy of metal carbonyls for bio-imaging and -sensing

Synthesis of magnetic Fe₃O₄–Au hybrids for sensitive SERS detection of cancer cells at low abundance

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A Rapid and Label‐free SERS Detection Method for Biomarkers in Clinical Biofluids

Cited by 31 publications

References 54 publications

Recent advances in M13 bacteriophage-based optical sensing applications

Recent advances in M13 bacteriophage-based optical sensing applications

Vibrational spectroscopy of metal carbonyls for bio-imaging and -sensing

Synthesis of magnetic Fe3O4–Au hybrids for sensitive SERS detection of cancer cells at low abundance

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Synthesis of magnetic Fe₃O₄–Au hybrids for sensitive SERS detection of cancer cells at low abundance