Abstract:This work reports a facile synthesis of magnetic hybrids (Fe 3 O 4 -Au hybrids) with high SERS activity for detecting a low abundance of cancer cells. We labeled Raman reporter molecule 4-aminothiophenol (4-ATP) and the antibody of CEA (anti-CEA) on Fe 3 O 4 -Au hybrid nanoparticles (anti-CEA/4-ATP/Fe 3 O 4 -Au) as SERS tags and anti-CEA-labeled Au NPs (anti-CEA/Au) as SERS-active substrates to improve detection sensitivity. In the presence of CEA positive-expressed cancer cells (such as non-small lung cancer … Show more
“…(i) multiplex detection capability due to spectral fingerprinting; (ii) not being vulnerable to photo-bleaching; and (iii) low cytotoxicity due to the usage of AuNPs [1]. 10.1080/20022727.2017.1373551-F0003Figure 3.Schematic illustration of formation of sandwich structure and SERS HOT spot with both SERS tags (anti-CEA/4-ATP/Fe 3 O 4 -Au NPs) and SERS – active substrate [41].
…”
Section: Nanoparticles and Raman Techniquesmentioning
confidence: 99%
“…Schematic illustration of formation of sandwich structure and SERS HOT spot with both SERS tags (anti-CEA/4-ATP/Fe 3 O 4 -Au NPs) and SERS – active substrate [41]. …”
Section: Nanoparticles and Raman Techniquesmentioning
confidence: 99%
“…The second is AuNPs attaching to targeted ligands (e.g. proteins, antibodies, aptamers, and small molecules), aiming for specific receptors, proteins and cell types [28,41,55,56]. Regarding the shape of AuNPs, some advantages were demonstrated when using star structures, but much work has also been done with spheres [4].…”
Section: Nanoparticles and Raman Techniquesmentioning
confidence: 99%
“…A SERS reporter was used in conjunction with anti-CEA labeled AuNPs (anti-CEA/Au) as SERS active substrate, to form hot spots on the surface of CEA positive-expressed cancer cells (e.g. A549), to improve detection sensitivity [41]. …”
Section: Nanoparticles and Raman Techniquesmentioning
confidence: 99%
“…Rhodamine6G was used as SERS reporter and a monoclonal antibody specific to PLCγ1 marker that is over expressed in HEK293 cancer cells. PLCγ1 in competition with GRB2 to attach to FGFR2, that provides a binding site for these two proteins, and determines prognosis of recurrence-free survival in lung adenocarcinoma [19,33,41]. Bei et alexplored AgNP-based, SERS applications for biochemical analysis of blood plasma samples and demonstrated the capability of this method to detect nasopharyngeal cancer [91].…”
Section: Detection Of Other Malignanciesmentioning
In vitro detection technique Raman spectroscopy (Rs), in one number times another Rs based expert ways of art and so on, are useful instruments for cancer discovery. top gave greater value to Raman spectroscopy sers is a relatively new careful way for in vitro and in vivo discovery that takes away bad points of simple Raman spectroscopy (Rs). Raman spectroscopy (RS) and in particular, multiple RS-based techniques are useful for cancer detection. Surface enhanced Raman spectroscopy (SERS) is a relatively new method for both in vitro and in vivo detection, which eliminates the drawbacks of simple RS. Using nanoparticles has elevated the sensitivity and specificity of SERS. SERS has the potential to increase sensitivity, specificity and spatial resolution in cancer detection, especially in cooperation with other diagnostic imaging tools such as magnetic resonance imaging (MRI) and PET-scan polyethylene terephthalate. Developing a hand held instrument for detecting cancer or other illnesses may also be feasible by using SERS. Frequently, novel nanoparticles are used in SERS. With a focus on nanoparticle utilization, we review the benefits of RS in cancer detection and related biomarkers. With a focus on nanoparticles utilizations, the benefits of RS in cancer detection and related biomarkers were reviewed. In addition, Raman applications to detect some of prevalent were discussed. Also more investigated cancers such as breast and colorectal cancer, multiple nanostructures and their possible special biomarkers, especially as SERS nano-tag have been reviewed. The main purpose of this article is introducing of most popular nanotechnological approaches in cancer detection by using Raman techniques. Moreover, have been caught up on detection and reviewed some of the most prevalent and also more investigated cancers such as breast, colorectal cancer, multiple intriguing nanostructures, especially as SERS nano-tag, special cancer biomarkers and related approaches. The main purpose of this article is to introduce the most popular nanotechnological approaches in cancer detection by using Raman techniques.
“…(i) multiplex detection capability due to spectral fingerprinting; (ii) not being vulnerable to photo-bleaching; and (iii) low cytotoxicity due to the usage of AuNPs [1]. 10.1080/20022727.2017.1373551-F0003Figure 3.Schematic illustration of formation of sandwich structure and SERS HOT spot with both SERS tags (anti-CEA/4-ATP/Fe 3 O 4 -Au NPs) and SERS – active substrate [41].
…”
Section: Nanoparticles and Raman Techniquesmentioning
confidence: 99%
“…Schematic illustration of formation of sandwich structure and SERS HOT spot with both SERS tags (anti-CEA/4-ATP/Fe 3 O 4 -Au NPs) and SERS – active substrate [41]. …”
Section: Nanoparticles and Raman Techniquesmentioning
confidence: 99%
“…The second is AuNPs attaching to targeted ligands (e.g. proteins, antibodies, aptamers, and small molecules), aiming for specific receptors, proteins and cell types [28,41,55,56]. Regarding the shape of AuNPs, some advantages were demonstrated when using star structures, but much work has also been done with spheres [4].…”
Section: Nanoparticles and Raman Techniquesmentioning
confidence: 99%
“…A SERS reporter was used in conjunction with anti-CEA labeled AuNPs (anti-CEA/Au) as SERS active substrate, to form hot spots on the surface of CEA positive-expressed cancer cells (e.g. A549), to improve detection sensitivity [41]. …”
Section: Nanoparticles and Raman Techniquesmentioning
confidence: 99%
“…Rhodamine6G was used as SERS reporter and a monoclonal antibody specific to PLCγ1 marker that is over expressed in HEK293 cancer cells. PLCγ1 in competition with GRB2 to attach to FGFR2, that provides a binding site for these two proteins, and determines prognosis of recurrence-free survival in lung adenocarcinoma [19,33,41]. Bei et alexplored AgNP-based, SERS applications for biochemical analysis of blood plasma samples and demonstrated the capability of this method to detect nasopharyngeal cancer [91].…”
Section: Detection Of Other Malignanciesmentioning
In vitro detection technique Raman spectroscopy (Rs), in one number times another Rs based expert ways of art and so on, are useful instruments for cancer discovery. top gave greater value to Raman spectroscopy sers is a relatively new careful way for in vitro and in vivo discovery that takes away bad points of simple Raman spectroscopy (Rs). Raman spectroscopy (RS) and in particular, multiple RS-based techniques are useful for cancer detection. Surface enhanced Raman spectroscopy (SERS) is a relatively new method for both in vitro and in vivo detection, which eliminates the drawbacks of simple RS. Using nanoparticles has elevated the sensitivity and specificity of SERS. SERS has the potential to increase sensitivity, specificity and spatial resolution in cancer detection, especially in cooperation with other diagnostic imaging tools such as magnetic resonance imaging (MRI) and PET-scan polyethylene terephthalate. Developing a hand held instrument for detecting cancer or other illnesses may also be feasible by using SERS. Frequently, novel nanoparticles are used in SERS. With a focus on nanoparticle utilization, we review the benefits of RS in cancer detection and related biomarkers. With a focus on nanoparticles utilizations, the benefits of RS in cancer detection and related biomarkers were reviewed. In addition, Raman applications to detect some of prevalent were discussed. Also more investigated cancers such as breast and colorectal cancer, multiple nanostructures and their possible special biomarkers, especially as SERS nano-tag have been reviewed. The main purpose of this article is introducing of most popular nanotechnological approaches in cancer detection by using Raman techniques. Moreover, have been caught up on detection and reviewed some of the most prevalent and also more investigated cancers such as breast, colorectal cancer, multiple intriguing nanostructures, especially as SERS nano-tag, special cancer biomarkers and related approaches. The main purpose of this article is to introduce the most popular nanotechnological approaches in cancer detection by using Raman techniques.
Cancer is the second most common cause of death in the world. The principal limitations thus far encountered in the clinical practice of probing cancer are diverse and include low sensitivity, time consumption, bulkiness, and cost. In this respect, nanomaterial (NM)-based sensing techniques are recognized as a superior alternative to efficiently resolve such limitations. A better understanding of NM-based sensing platforms is thus important so that these novel avenues can easily be explored for clinical applications. These platforms have the merits of high sensitivity, high specificity, rapid response, and easy-to-read signals. This review offers a comprehensive survey of NM-based advanced cancer-sensing techniques and will help the scientific community establish optimum sensing strategies based on an accurate assessment of the interactions between cancer biomarkers and NM-based platforms.but are not limited to, carcinoma, sarcoma, leukemia, lymphoma, non-Hodgkin lymphoma, myeloma, central nervous system cancer, lung and bronchus cancer, colon cancer, rectum cancer, prostate cancer, bladder cancer, kidney and renal pelvis cancer, endometrial cancer, pancreatic cancer, liver cancer, and thyroid cancers. [1] Moreover, the most commonly occurring cancers tend to differ by common criteria like sex and age. For example, men are at risk for prostate, lung, and colorectal cancer, women are at risk for breast, lung and colorectal cancer, and children are at risk for leukemia, brain tumors, and lymphoma. The severity of the cancer depends upon the development level or staging of the disease. [2] Likewise, the type of treatment required to treat the cancer is determined by the staging of the disease.The more severe condition of cancer is metastasis, i.e., the uncontrolled growth of cells that invade surrounding tissues. The metastasis condition of cancer can affect any organ of the body. [3] As per the WHO 2010 report on cancer, the lives of 30% patients could have been saved if the cancer was diagnosed earlier. The detection of cancer is not easy because the immune system does not necessarily consider cancerous cells as foreign bodies. However, a few changes (such as altered biomarker levels and the presence of cancerous cells in biological fluids) can be detected. [4] In general, cancer biomarkers are overexpressed proteins present in blood/serum and at the cancer cell surface. The accurate and efficient detection of these biomarkers can be useful for cancer detection. The major problem with biomarkers is their low level in the body during the earlier stages of cancer. Hence, an efficient sensor/device capable of detecting these molecules even at very low levels, if developed, could help the clinician efficiently diagnose cancer cells in the human body.The sensing techniques presently used by clinicians suffer from several limitations; specifically, they are time consuming, bulky, have low sensitivity, and are expensive. Nanomaterial (NM)-based techniques have become an important alternative to the laborious conventional...
Raman‐Spektroskopie ist eine aufstrebende Technik in der Analyse und Bildgebung von Biomaterialien, da sie in der Lage ist, einen einzigartigen spektroskopischen Fingerabdruck zu erzeugen. Die Darstellung von Zellen und Geweben durch Raman‐Mikrospektroskopie stellt einen zerstörungsfreien und markerfreien Ansatz dar. Dabei tragen alle Komponenten zu den Raman‐Signalen bei, die komplexe spektrale Signaturen liefern. Verfahren wie Resonanz‐Raman‐Streuung und oberflächenverstärkte Raman‐Streuung verstärken die Signale und ergeben einfachere Spektren. Raman‐aktive Markierungen können eingeführt werden, um die Spezifität und die Multimodalität zu erhöhen. Nichtlineare kohärente Raman‐Streuung bietet höhere Empfindlichkeiten, die eine schnelle Abbildung größerer Probenflächen ermöglichen. Schließlich eröffnen faserbasierte Bildgebungsverfahren den Weg zu In‐vivo‐Anwendungen der Raman‐Spektroskopie. Dieser Aufsatz fasst die Grundlagen der medizinischen Raman‐Bildgebung und ihre Fortschritte seit 2012 zusammen.
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