A quick approach for medetomidine enantiomer determination in dog plasma by chiral liquid chromatography–tandem mass spectrometry and application to a pharmacokinetic study

Abstract

In the present study, a rapid, sensitive and high-throughput liquid chromatographytandem mass spectrometry (LC-MS/MS) method for the determination of medetomidine enantiomers in dog plasma was developed and validated. The separation and individual quantification of chiral compounds can be a tricky task in

Enantiospecific pharmacokinetics of intravenous dexmedetomidine in beagles

“…The calculated limit of detection (LOD) was 0.01 ng/mL, and the lower limit of quantification (LLOQ) was 0.1 ng/mL. The intraday accuracy and precision were 4.8 [3.1–5.8]% and 6.2 [4.4–7.0]% for dexmedetomidine, and 2.2 [2.0–3.6]% and 6.7 [6.1–7.0]% for levomedetomidine, respectively (Bardhi et al, 2021).…”

“…Blood was then centrifuged for 10 min at 1500 g at 10°C, the plasma extracted and then stored frozen at −80°C until analysis. The quantification of dexmedetomidine and levomedetomidine was performed by chiral LC-MS/MS as described previously (Bardhi et al, 2021). The analytical method was validated in accordance with EMEA/CHMP/ EWP/192217/2009 guidelines at the beginning of the experiment (EMA., 2011).…”

“…The analytical method was validated in accordance with EMEA/CHMP/ EWP/192217/2009 guidelines at the beginning of the experiment (EMA., 2011). The details of the quantification methodology are provided elsewhere (Bardhi et al, 2021). The calculated limit of detection (LOD) was 0.01 ng/mL, and the lower limit of quantification (LLOQ) was 0.1 ng/mL.…”