A quantitative evaluation of sealing ability of 4 obturation techniques by using a glucose leakage test

Xu, Qiong; Ling, Junqi; Cheung, Gary Shun Pan; Yan, Hu

doi:10.1016/j.tripleo.2007.05.014

Cited by 35 publications

(37 citation statements)

References 29 publications

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“…The relevance of the proposed technique is further confirmed by comparing our results with those of other studies, using UV-VIS. 13,14,21,22,23,24,25,26,27,28,29 HPLC-MS/MS is able to detect concentrations at least 1,000 times lower. These data underscore the superiority of HPLC-MS/MS over UV-VIS detectors, and suggest that the new quantification method is appropriate for tracer detection, even at very low concentrations.…”

Section: Discussionmentioning

confidence: 99%

Applying liquid chromatography-tandem mass spectrometry to assess endodontic sealer microleakage

et al. 2015

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The objective of this study was to describe a new method for the quantitative analysis of a microleakage of endodontic filling materials. Forty extracted single-rooted teeth were randomly divided into three experimental groups. After root canal shaping, the experimental groups were filled using the lateral condensation technique with the Epiphany system (G1), with gutta-percha + Sealapex (G2), and with gutta-percha + AH Plus (G3). Each root was mounted on a modified leakage testing device, and caffeine solution was used as a tracer (2000 ng mL -1 , pH 6.0), applied in the coronal direction towards the tooth apex, creating a hydrostatic pressure of 2.55 kPa. Presence of caffeine in the receiving solution was measured after 10, 30, and 60 days, using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). None of the groups presented microleakage at 10 days. At 30 days, G2 and G3 showed similar infiltration patterns (means: 16.0 and 13.9 ng mL -1 , respectively), whereas G1 showed significantly higher values (mean: 105.2 ng mL -1 ). At 60 days, leakage values were 182.6 ng mL -1 for G1, 139.0 ng mL -1 for G2, and 53.5 ng mL -1 for G3. AH Plus showed the best sealing ability and HPLC-MS/MS showed high sensitivity and specificity for tracer quantification.

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Section: Discussionmentioning

confidence: 99%

Applying liquid chromatography-tandem mass spectrometry to assess endodontic sealer microleakage

et al. 2015

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“…Desta maneira utilizaram-se dentes com canais únicos e retos, que tiveram suas coroas eliminadas e as raízes mantidas com 12 mm de comprimento. Um consenso entre os pesquisadores ocorre em relação à utilização de dentes com canais únicos e retos, mas uma pequena variação no comprimento pode ser observada, variando de 11 mm (XU et al, 2007), 12 mm (DE DEUS et al, 2008a;KAYA et al, 2007;SLUIS et al, 2007), 14 mm (OZOK et al, 2008 até 15 mm (SHEMESH; WU; WESSELINK, 2006;SOUZA et al, 2008;XU et al, 2005).…”

Section: Resultsunclassified

“…Durante a instrumentação dos canais radiculares a manutenção da patência foi realizada com a utilização de um instrumento número 15 (OZOK et al, 2008;XU et al, 2005;XU et al, 2007) e a irrigação entre o uso de cada instrumento com 2 mL de solução de hipoclorito de sódio a 2,5% (KAYA et al, 2007;XU et al, 2005;XU et al, 2007). A utilização de EDTA 17% na irrigação também tem sido empregada em diversas pesquisas (KAYA et al, 2007;OZOK et al, 2008;XU et al, 2005;XU et al, 2007).…”

Section: Resultsunclassified

“…A utilização de EDTA 17% na irrigação também tem sido empregada em diversas pesquisas (KAYA et al, 2007;OZOK et al, 2008;XU et al, 2005;XU et al, 2007).…”

Section: Resultsunclassified

“…Os espécimes do grupo controle negativo permaneceram com o material obturador em todo o canal radicular e tiveram as raízes impermeabilizadas com esmalte de unhas (KAYA et al, 2007;OZOK et al, 2008;WESSELINK, 2006;SLUIS et al, 2007;XU et al, 2005 A utilização da hifenação da técnica de CLAE-EM/EM nos possibilitou quantificar concentrações do agente traçador muito abaixo dos valores já publicados (DE-DEUS et al, 2008;KAYA et al, 2007;OZOK et al, 2008;SHEMESH et al, 2006;SLUIS et al, 2007;SOUZA et al, 2008;XU et al, 2005;XU et al, 2007;ZOU et al, 2007). Foi realizado um estudo comparativo do limite mínimo de detecção da cafeína pelo CLAE-EM/EM, com um Espectrofotômetro UV-ViS, onde este limite de detecção foi de 2000 ng/mL de cafeína, valor esse acima do limite máximo de leitura da curva de calibração obtido através do CLAE-EM/EM (10 -1800 ng/mL).…”

Section: Resultsunclassified

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Análise da capacidade seladora de materiais obturadores endodônticos utilizando a técnica de cromatografia líquida de alta eficiência acoplada à espectrometria de massas

Michelotto¹

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Percentage of gutta‐percha in mesial canals of mandibular molars obturated by lateral compaction or single cone techniques

Rodrigues

Bonetti-Filho

Faria

et al. 2012

Microscopy Res & Technique

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The aim of this study was to compare the percentage of gutta-percha (PGP) in mesial root canals of mandibular molars obturated with LC (Lateral Compaction) or SC (Single Cone) ProTaper Universal System techniques at different levels of the root. Mesial root canals of 20 human permanent molars with similar anatomical characteristics were instrumented using the ProTaper Universal rotary system technique until the F2 instrument, with 20 canals filled by SC ProTaper Universal technique and 20 canals by the LC technique. The mesial roots were sectioned transversely to 3, 5, and 7 mm from the root apex. Digital images of specimens were obtained at MIC-D digital microscope in increases of 30 to 35X. The gutta-percha area was measured using ImageTool software. Data were analyzed using two-way ANOVA and Bonferroni test (α = 0.05). The SC technique provided greater PGP than the LC technique in the apical third (3 mm) (P < 0.001). In the other thirds (5 and 7 mm) there was no statistical difference between the two techniques regarding the PGP (P > 0.05). There was no statistically significant difference between thirds of the root canal for both techniques (P > 0.05). It was concluded that SC technique provided greater PGP than the LC technique in the apical third of mesial root canals of mandibular molars. There was no difference between the two techniques regarding the PGP in the cervical and middle thirds.

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A quantitative evaluation of sealing ability of 4 obturation techniques by using a glucose leakage test

Cited by 35 publications

References 29 publications

Applying liquid chromatography-tandem mass spectrometry to assess endodontic sealer microleakage

Applying liquid chromatography-tandem mass spectrometry to assess endodontic sealer microleakage

Análise da capacidade seladora de materiais obturadores endodônticos utilizando a técnica de cromatografia líquida de alta eficiência acoplada à espectrometria de massas

Percentage of gutta‐percha in mesial canals of mandibular molars obturated by lateral compaction or single cone techniques

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