A quantitative enzyme-linked immunosorbent assay (ELISA) for the immunodiagnosis of neurocysticercosis using a purified fraction from Taenia solium cysticerci

Evaluation of two Taenia solium cysticercal antigenic preparations (vesicular fluid and a glycoprotein fraction with affinity for lentil lectin) for the immunodiagnosis of neurocysticercosis by enzyme-linked immunosorbent assay (ELISA)

“…One CSF sample from a patient with toxoplasmosis cross-reacted with the LLaGp fraction. Other studies have also shown that components of T. solium cysticerci are recognized by CSF and/ or serum samples from patients with toxoplasmosis 17,31 .…”

Crude antigen from Taenia crassiceps cysticercus used as heterologous antigen in ELISA and in EITB for neurocysticercosis diagnosis of patients from Paraná-Brazil

“…The results of an immunodiagnostic test for NCC could increase the probability of getting the correct diagnosis. A step further in this direction could be to use crude antigen from C. cellulosae (Silva et al, 2000;Iudici Neto et al, 2007;Parija et al, 2007). However, it is very difficult to obtain enough quantities of an antigen as that depends either on finding naturally infected swine or infecting them experimentally with T. solium eggs.…”

Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for immunodiagnosis of neurocysticercosis using ELISA on cerebrospinal fluid samples

“…The assay was done as previously described 23 , with the following modifications: the cysticercal antigens were diluted to 3µg of protein/ml in 0.1M carbonate-bicarbonate buffer, at pH 9.5, prior to sensitizing U-bottomed ELISA plates. The CSF samples and the CYSP were diluted to 1:2 and 1:100, respectively, in PBS-Tween containing 1% bovine serum albumin.…”