2007
DOI: 10.1590/s0037-86822007000200002
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Evaluation of Taenia solium and Taenia crassiceps cysticercal antigens for immunodiagnosis of neurocysticercosis using ELISA on cerebrospinal fluid samples

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Cited by 18 publications
(22 citation statements)
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“…VF was obtained as previously described 17 . Briefly, the parasites were ruptured individually in petri dishes using two needles and the VF released was collected with a Pasteur pipette and transferred to centrifuges tubes.…”
Section: Cysticercal Antigensmentioning
confidence: 99%
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“…VF was obtained as previously described 17 . Briefly, the parasites were ruptured individually in petri dishes using two needles and the VF released was collected with a Pasteur pipette and transferred to centrifuges tubes.…”
Section: Cysticercal Antigensmentioning
confidence: 99%
“…The WEC was obtained as previously described 15,17 , with few modifications. Briefly, parasites maintained at -80 o C were thawed and resuspended in sterile 0.15 M phosphate-buffered saline (PBS), pH 7.2 (1 volume of parasites to 3 volumes of PBS containing PMSF and leupeptin at the concentrations indicated above) and the material then homogenized in an ice-water bath using a Polytron homogenizer equipped with a PT-20 ST probe (three 30 s pulses at speed 3, with 30 s intervals between pulses).…”
Section: Cysticercal Antigensmentioning
confidence: 99%
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“…Reviewing the literature, it appears that the ELISA method has shown different sensitivity and specificity scores [17][18][19][20][21][22][23][24][25][26][27][28] . This study determined a sensitivity of 31.8% and specificity of 100% for the ELISA method in CSF in relation to diagnosing neurocysticercosis, which implies low sensitivity of ELISA for diagnosing neurocysticercosis, with a 68.3% rate of false negative.…”
Section: Carvalho-junior Rm Et Al -Enzyme Immunoassay For Diagnosis Omentioning
confidence: 99%