Crude antigen from Taenia crassiceps cysticercus used as heterologous antigen in ELISA and in EITB for neurocysticercosis diagnosis of patients from Paraná-Brazil

Minozzo, João Carlos; Moura, Juliana de; Almeida, Sérgio Monteiro de; Soccol, Vanete Thomaz

doi:10.1590/s1516-89132008000600007

Cited by 4 publications

(4 citation statements)

References 36 publications

(43 reference statements)

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“…Although PCR, targeted at a highly repetitive element (pTsol9) of the T. solium genome, has been reported as a molecular diagnostic test taking CSF of radiologically and clinically confirmed NCC patients with a positivity of 96.7 % (Almeida et al, 2006) and 90 -100 % (Michelet et al, 2011) respectively. Detection of highly specific circulating antigens or specific DNA in CSF can be important in immunocompetent NCC patients who turn seronegative in ELISA (Michelet et al, 2011;Ito et al, 2006). PCR could be useful as an alternative diagnostic test for NCC during the non-availability of imagery techniques.…”

Section: Discussionmentioning

confidence: 99%

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Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India)

Satyaprakash,

Khan,

Zade

et al. 2023

Helminthologia

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SummaryNeurocysticercosis (NCC), one of the most important neuroparasitic diseases in humans, is caused byCysticercus cellulosae, the metacestode stage of digenetic zoonotic cestodeTaenia solium. The present study aims at the detection of anti-cysticercus antibodies in the sera of epileptic patients (n=26) visiting a tertiary care hospital in Nagpur, Maharashtra state, India, by an in-house developed indirect IgG-ELISA and enzyme-linked immunoelectro transfer blot (EITB) assay using different antigens (namely, Whole Cyst Antigen (WCA), Cystic Fluid Antigen (CFA), Scolex Antigen (SA), Excretory-Secretory Antigen (ESA) and Membrane-Body Antigen (MBA)) prepared fromT. soliummetacestodes to find out the status of NCC. An attempt has also been made for molecular detection of NCC from blood samples of those patients by Polymerase Chain Reaction (PCR) assay targeted atlarge subunit rRNAgene ofT. solium. The IgG ELISA level of anti-cysticercus antibodies against WCA, CFA, SA, ESA and MBA antigens were as follows: 19.23 %, 23.07 %, 38.46 %, 30.76 % and 15.38 %. The seroreactivity to CFA, SA and ESA was found in equal proportions in patients with ring-enhancing lesions. In the EITB assay, the lower and medium molecular weight protein bands of SA and ESA were immunodominant compared to the higher WCA and CFA peptides. PCR positivity could be observed in 34.6 % (9/26) of the patients under study. It is the first report of detecting NCC among epileptic patients of the Nagpur region of Maharashtra state in India using serological and molecular tools.

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Section: Discussionmentioning

confidence: 99%

“…The reaction was developed with O-phenylene-diamine dihydrochloride (OPD) (Sigma Aldrich, USA), and the absorbance was measured at 492 nm with an ELISA reader (Thermo Fisher, USA). A positive ELISA result was defined as two standard deviations above the negative sera’s optical density (OD) mean value ( Minozzo et al ., 2008 ).…”

Section: Methodsmentioning

confidence: 99%

Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India)

Satyaprakash,

Khan,

Zade

et al. 2023

Helminthologia

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show abstract

“…The reaction was developed with O-phenylene-diamine dihydrochloride (OPD) (Sigma Aldrich, USA) and the absorbance was measured at 492 nm with ELISA reader (Multiskan Go, Thermo Fisher, Finland). A positive ELISA result was defined as two standard deviations above the mean OD value of negative sera ( 21 ).…”

Section: Methodsmentioning

confidence: 99%

Optimization of In-House Indirect-ELISA & EITB Assays Employing Cysticercus cellulosae Antigens for Serological Detection and PCR Assays for Molecular Detection of Porcine Cysticercosis

Satyaprakash¹,

Khan²,

Chaudhari³

et al. 2023

IJPA

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Background: Porcine cysticercosis, caused by metacestodes of Taenia solium is an important neglected zoonosis. We evaluated the presence of anti-cysticercal antibodies and T. solium specific DNA in pig sera and blood samples respectively collected from Maharashtra, India. Methods: A total of three antigens (Scolex Antigen (SA), Membrane Body Antigen (MBA) and Excretory-Secretory Antigen (ESA)) were prepared from metacestodes of T. solium and employed in an in-house developed indirect-IgG ELISA for serological screening of 1000 porcine sera samples at Department of Veterinary Public Health, Nagpur Veterinary College, Maharashtra, India. The ELISA positive sera samples were subjected to EITB Assay for detection of immunodominant peptides. An effort has been made for molecular detection of porcine cysticercosis by PCR assay targeting large subunit rRNA gene of T. solium from blood samples of the corresponding ELISA-positive pigs. Results: The overall seroprevalence of porcine cysticercosis employing SA, MBA and ESA was 12.6%, 8.7% and 12.5% respectively. The lower and medium molecular weight peptides were the most frequently recognised in EITB assay. The numbers of bands recognised in EITB assay were observed to be proportionate with the corresponding ELISA O.D. values. An amplification product of 286 bp was observed in 22.98% (20/87), 30.35% (30/99) and 17.14% (12/70) of the sero-positive samples against SA, ESA and MBA respectively. Conclusion: EITB still remains the gold standard serodiagnosis test for cysticercosis. The inclusion of a greater number of positive samples and purification of antigens may improve the diagnostic efficacy of the tests.

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“…Indirect ELISA (Minozzo et al, 2008): The tests and reference values of the positive and negative sera were optimized after checkerboard titration (Sigma, USA). All reagents were used as 100µl/well, tested sera were diluted with PBS as 1:100, and Horseradish peroxidase-conjugated anti-human &/or anti-pig IgG was diluted as 1:2000.…”

Section: Introductionmentioning

confidence: 99%

Antigens From Pig Cysticercosis and Their Valuability in Diagnosis of Human Cysticercosis

Abdel-Radi

El-Hafez

Morsy

2020

Journal of the Egyptian Society of Parasitology

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Crude antigen from Taenia crassiceps cysticercus used as heterologous antigen in ELISA and in EITB for neurocysticercosis diagnosis of patients from Paraná-Brazil

Abstract: Neurocysticercosis (NCC), the cerebral presence of

Cited by 4 publications

References 36 publications

Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India)

Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India)

Optimization of In-House Indirect-ELISA & EITB Assays Employing Cysticercus cellulosae Antigens for Serological Detection and PCR Assays for Molecular Detection of Porcine Cysticercosis

Antigens From Pig Cysticercosis and Their Valuability in Diagnosis of Human Cysticercosis

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