A protein delivery system: biodegradable alginate–chitosan–poly(lactic-co-glycolic acid) composite microspheres

Zheng, Caihong; J, Gao; Zhang, Ye-Ping; Liang, Wenquan

doi:10.1016/j.bbrc.2004.09.007

Cited by 119 publications

(55 citation statements)

References 22 publications

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“…54 To improve the drug-encapsulation properties of PLGA NPs, a possible strategy consists in the modification of the PLGA surface by coating with stabilizing hydrophilic bioadhesive polymers or surfactants. 55 In the present study, we highlighted that cationic CS and anionic Alg coating on PLGA NPs determined a significant improvement in RSV entrapment efficiency.…”

Section: Particle-size Analysis and Zeta Potentialmentioning

confidence: 99%

Development of novel cationic chitosan- and anionic alginate–coated poly(D,L-lactide-co-glycolide) nanoparticles for controlled release and light protection of resveratrol

Sanna¹,

Roggio²,

Siliani³

et al. 2012

IJN

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Background:Resveratrol, like other natural polyphenols, is an extremely photosensitive compound with low chemical stability, which limits the therapeutic application of its beneficial effects. The development of innovative formulation strategies, able to overcome physicochemical and pharmacokinetic limitations of this compound, may be achieved via suitable carriers able to associate controlled release and protection. In this context, nanotechnology is proving to be a powerful strategy. In this study, we developed novel cationic chitosan (CS)-and anionic alginate (Alg)-coated poly(d,l-lactide-co-glycolide) nanoparticles (NPs) loaded with the bioactive polyphenolic trans-(E)-resveratrol (RSV) for biomedical applications. Methods: NPs were prepared by the nanoprecipitation method and characterized in terms of morphology, size and zeta potential, encapsulation efficiency, Raman spectroscopy, swelling properties, differential scanning calorimetry, and in vitro release studies. The protective effect of the nanosystems under the light-stressed RSV and long-term stability were investigated. Results: NPs turned out to be spherical in shape, with size ranging from 135 to about 580 nm, depending on the composition and the amount of polyelectrolytes, while the encapsulation efficiencies increased from 8% of uncoated poly(d,l-lactide-co-glycolide) (PLGA) to 23% and 32% of Alg-and CS-coated PLGA NPs, respectively. All nanocarriers are characterized by a biphasic release pattern, and more effective controlled release rates are obtained for NPs formulated with higher polyelectrolyte concentrations. Stability studies revealed that encapsulation provides significant protection against light-exposure degradation, by reducing the trans-cis photoisomerization reaction. Moreover, the nanosystems are able to prevent the degradation of trans isoform and the leakage of RSV from the carrier for a period of 6 months. Conclusion: Our findings indicated that the newly developed CS-and Alg-coated PLGA NPs are suitable to be used for the delivery of bioactive RSV. The encapsulation of RSV into optimized polymeric NPs provides improved drug loading, effective controlled release, and protection against light-exposure degradation, thus opening new perspectives for the delivery of bioactive related phytochemicals to be used for (nano)chemoprevention/chemotherapy.

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Section: Particle-size Analysis and Zeta Potentialmentioning

confidence: 99%

Development of novel cationic chitosan- and anionic alginate–coated poly(D,L-lactide-co-glycolide) nanoparticles for controlled release and light protection of resveratrol

Sanna¹,

Roggio²,

Siliani³

et al. 2012

IJN

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“…Among these, PLGA microsphere/hydrogel combination systems could not only provide entrapped proteins a more 'friendly' environment, but also retard the initial burst release. Alginate and chitosan are the two most commonly used hydrophilic polymers, but an obvious limitation was that the size of composite microspheres was too large, for example, alginate particles were embedded into PLGA microparticles and the resulting composite microparticles had a d mv of ∼ 65 µm (Schoubben et al, 2009), alginate-PLGA composite microspheres of 19-73 µm diameter (LuzardoAlvarez et al, 2005), and alginate-chitosan-PLGA composite ones of 30 µm diameter (Zheng et al, 2004a), compared with the convenient PLGA microspheres of ∼ 5 µm. To maintain the size of the microparticles, we put more attention on those slightly modified methods.…”

Section: Introductionmentioning

confidence: 99%

A one-step modified method to reduce the burst initial release from PLGA microspheres

Zheng

Liang

2010

Drug Delivery

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“…A síntese de micelas de albumina e colágeno foi realizada de acordo com a metodologia descrita por Zheng et al (2004), com algumas modificações.…”

Section: Síntese E Preparo Das Micelasunclassified

Application and viability of L. acidophilus, Bifidobacterium and S. thermophilus microencapsulated in soy frozen yogurt

Gon

Gonzalez

Sereia

2015

REBRAPA

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Resumo: A soja é um alimento funcional e se caracteriza por oferecer vários efeitos benéficos à saúde permitindo a ampliação da pesquisa para desenvolvimento de produtos funcionalizados como substitutos alternativos de derivados lácteos. Este estudo teve por objetivo elaborar a partir do extrato aquoso de soja um frozen yogurt enriquecido com cálcio e adicionado de uma cultura mista contendo Lactobacillus acidophilus, Bifidobacterium e Streptococcus thermophilus microencapsuladas. As micelas foram preparadas a partir de albumina e colágeno com a adição da cultura probiótica superativada. O frozen yogurt foi preparado à base de extrato aquoso de soja. As micelas de albumina e colágeno foram capazes de proteger os microrganismos probióticos do frio mantendo-os viáveis durante o armazenamento. As micelas promoveram a obtenção de um produto com características físicas, químicas e sensoriais adequadas a de um "gelado comestível", mostrando que o frozen yogurt de soja pode ser considerado uma boa opção de produto para o mercado.Palavras-chaves: Alimento funcional; Micelas; Probióticos; Gelado Comestível. Application and viability of L. acidophilus, Bifidobacterium and S. thermophilus microencapsulated in soy frozen yogurt:Soybean is a functional food and it is characterized by providing several beneficial health effects allowing the expansion of research and development of functionalized products as alternative substitutes of dairy products. This study aimed to elaborate frozen yogurt enriched with calcium and added a mixed culture containing Lactobacillus acidophilus, Bifidobacterium and Streptococcus thermophilus microencapsulated, from aqueous extract of soybean. The micelles were prepared from albumin and collagen with the addition of over activated probiotic culture. The frozen yogurt was prepared on the basis of aqueous extract of soybean. The micelles of albumin and collagen were able to protect the probiotic microorganisms from cold keeping them viable for storage. The micelles were able to obtain a product with adequate physical, chemical and sensory characteristics for an edible frozen, what shows that soy frozen yogurt may be a good choice of product for the market. (CDR, 1999;TOLEDO, et al., 2007). Keywords

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A protein delivery system: biodegradable alginate–chitosan–poly(lactic-co-glycolic acid) composite microspheres

Cited by 119 publications

References 22 publications

Development of novel cationic chitosan- and anionic alginate–coated poly(D,L-lactide-co-glycolide) nanoparticles for controlled release and light protection of resveratrol

Development of novel cationic chitosan- and anionic alginate–coated poly(D,L-lactide-co-glycolide) nanoparticles for controlled release and light protection of resveratrol

A one-step modified method to reduce the burst initial release from PLGA microspheres

Application and viability of L. acidophilus, Bifidobacterium and S. thermophilus microencapsulated in soy frozen yogurt

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A protein delivery system: biodegradable alginate–chitosan–poly(lactic-co-glycolic acid) composite microspheres

Cited by 119 publications

References 22 publications

Development of novel cationic chitosan- and anionic alginate&ndash;coated poly(D,L-lactide-co-glycolide) nanoparticles for controlled release and light protection of resveratrol

Development of novel cationic chitosan- and anionic alginate&ndash;coated poly(D,L-lactide-co-glycolide) nanoparticles for controlled release and light protection of resveratrol

A one-step modified method to reduce the burst initial release from PLGA microspheres

Application and viability of L. acidophilus, Bifidobacterium and S. thermophilus microencapsulated in soy frozen yogurt

Contact Info

Product

Resources

About

Development of novel cationic chitosan- and anionic alginate–coated poly(D,L-lactide-co-glycolide) nanoparticles for controlled release and light protection of resveratrol

Development of novel cationic chitosan- and anionic alginate–coated poly(D,L-lactide-co-glycolide) nanoparticles for controlled release and light protection of resveratrol