A promising wound dressing based on alginate hydrogels containing vitamin D3 cross-linked by calcium carbonate/d-glucono-δ-lactone

Abstract

In the present study, we fabricated vitamin D 3 -loaded alginate hydrogel and assessed its wound healing capability in the animal model. The various concentrations of vitamin D3 were added to the pre-dissolved sodium alginate in deionized water and cross-linked by calcium carbonate in combination with d-glucono-δ-lactone. The microstructure, swelling behavior, weight loss, hemo-and cytocompatibility of the fabricated hydrogels were evaluated. In the last stage, the therapeutic efficacy of the prepared hydrogels was evaluated in the full-thickness dermal wound model. The scanning electron microscopy images showed that the prepared hydrogel was highly porous with the porosity of 89.2 ± 12.5% and contained the interconnected pores. Weight loss assessment showed that the prepared hydrogel is biodegradable with the weight loss percentage of about 89% in 14 days. The results showed that the prepared hydrogels were hemo-and cytocompatible. The animal study results implied that alginate hydrogel/3000 IU vitamin D 3 group exhibited the highest wound closure present which was statistically significant than the control group (p < 0.05). Moreover, the histological examinations revealed that hydrogel containing 3000 IU vitamin D3 had the best performance and induced the highest re-epithelialization and granular tissue formation. All in all, this study suggests that alginate hydrogels with 3000 IU vitamin D 3 can be exploited as a potential wound dressing in skin tissue engineering.

A tailored polylactic acid/polycaprolactone biodegradable and bioactive 3D porous scaffold containing gelatin nanofibers and Taurine for bone regeneration

“…The weight loss measurement was carried out according to our previous paper 43 . Briefly, the prepared scaffolds were gently cut into 20 mm diameter disks, weighed, and immersed in the glass test tubes filled with 15 ml of PBS containing 250 ng/ml amphotericin B, 100 unit/ml penicillin, and 100 µg/ml of streptomycin.…”

“…The compression test method was applied to evaluate the mechanical properties of the scaffolds 43 . Three dried cylindrical samples of each scaffold (height and diameter of 20 mm and 10 mm, respectively) were prepared and evaluated using a mechanical testing machine (Santam, Karaj, Iran) at a crosshead speed of 0.5 mm/min.…”

“…The absorbance was attributed to the hemoglobin leaked from red blood cells (RBC) and hemolysis degree was calculated using Eq. ( 3 ) 43 : where D t , D nc, and D pc are the absorbance of the sample, the absorbance of the negative control, and the absorbance of the positive control. The negative and positive controls were 0.2 ml of blood diluted with normal saline and deionized water, respectively.…”

“…The results were statistically analyzed by Minitab (version 17, Minitab Inc., https://www.minitab.com/en-us/ , State College, USA) software using Student’s t-test and the data were expressed as the mean ± standard deviation (SD) 43 . In all evaluations, p < 0.05 was considered as statistically significant.…”

Chitosan hydrogel loaded with Aloe vera gel and tetrasodium ethylenediaminetetraacetic acid (EDTA) as the wound healing material: in vitro and in vivo study

“…Various types of biomaterials have been developed for wound healing applications, such as ointments, foams, transparent films, gauze, hydrocolloids, micro/nanofibers, and hydrogels 9,10 . Among them, hydrogel‐based wound dressings offer promising advantages, such as high water uptake and retention, keeping the wound moist, no wound adherence, promoting autolysis debridement, and facilitating epithelial migration and granulation 10,11 . Moreover, according to the theory of moist wound healing, the epithelialization occurs at twice the rate compared with a dry wound 12,13 .…”