A Preparation of Cow's Late Colostrum Fraction Containing αs1-Casein Promoted the Proliferation of Cultured Rat Intestinal IEC-6 Epithelial Cells

Abstract

Colostrum is a complex mixture of bioactives that promotes neonate growth. Recently, we have found by in vivo study that skimmed, sterilized, and concentrated bovine late colostrum (SCBLC), obtained from a Holstein herd on days 6-7 after parturition, had an ability to maintain intestinal integrity. In the present study we investigated effects of SCBLC on rat intestinal IEC-6 cell proliferation in vitro. A fraction containing αs1-casein was found to have a robust stimulation effect as compared to other protein fractions from SCBLC and even the αs1-casein fraction from milk from other Holstein herds. Furthermore, the SCBLC αs1-casein molecule demonstrated not only slightly slower mobility on both SDS- and native-PAGE than other bovine milk αs1-caseins, but also a peculiar conformation reminiscent of moltenglobule in the circular dichroism spectrum. These findings may be of relevant to the competence of SCBLC to preserve intestinal integrity.

An in depth proteomic analysis based on ProteoMiner, affinity chromatography and nano-HPLC–MS/MS to explain the potential health benefits of bovine colostrum

“…Most of the beneficial effects of BC have been ascribed to the protein components. For instance immunoglobulins, that represent the main protein class of colostrum, have direct antimicrobial and endotoxinneutralizing effects throughout the alimentary tract, playing a direct role in the defense of the gastro-intestinal (GI) tract [8][9][10][11]. BC also has growth-promoting effects on calf gut epithelial cells as well as on human epithelial cells, which is attributable to the combination of several growth factors, including insulin-like growth factors (IGFs), basic fibroblast growth factors and plateletderived growth factor [5,12,13].…”

“…Based on the beneficial effects, as reported above, BC has been included in several health products making various claims such as supporting the immune system, treating gastro-intestinal infection and for the maintenance of the skin and mucous membranes integrity, favoring their healing [9,13] and also for the prevention and treatment of vaginal dryness [14].…”

Effect of bovine colostrum intake on growth, reproductive parameters and survival in red kids

“…Colostrum is a high‐value product in the diet of young suckling animals (Cairangzhuoma et al., ; Xu et al., ) in that it contains elements required for the survival and growth of the young animals (Sun et al., ). In general, the period from birth to weaning is a critical step for many mammals.…”