An in depth proteomic analysis based on ProteoMiner, affinity chromatography and nano-HPLC–MS/MS to explain the potential health benefits of bovine colostrum

Altomare, Alessandra; Fasoli, Elisa; Colzani, Mara; Parra, Ximena Maria Paredes; Ferrari, Marina G.; Cilurzo, Francesco; Rumio, Cristiano; Cannizzaro, Luca; Carini, Marina; Righetti, Pier Giorgio; Aldini, Giancarlo

doi:10.1016/j.jpba.2016.01.013

Cited by 19 publications

(17 citation statements)

References 33 publications

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“…Most of the beneficial effects of BC have been ascribed to the protein components consisting of 1786 proteins, as recently found by our team [5]. For instance immunoglobulins, which represent the main protein class of colostrum, have direct antimicrobial and endotoxin-neutralizing effects throughout the alimentary tract, playing the role of a direct GI tract defence [6].…”

Section: Introductionmentioning

confidence: 71%

“…By using an in depth proteomic and bioinformatic approach, we recently identified 1786 proteins and among these we selected protein clusters that on the basis of their biological activity can explain some claimed health benefits. For instance, BC is used as a functional ingredient in products claimed to help the wound healing process and we identified 93 proteins involved in the different steps of the wound healing process [5].…”

Section: Resultsmentioning

confidence: 99%

“…3c), were assigned to proteins with an MW from 112 kDa to 163 kDa. Several abundant proteins identified in colostrum fall into this MW range such as two different isoforms of xanthine dehydrogenase/oxidase (146.6 kDa), thrombospondin-1/2 (129 kDa), complement factor H (140 kDa), two different isoforms of alpha-mannosidase (130 kDa), G-protein coupled receptor 126 (132 kDa), aldehyde oxidase (147 kDa) [5]. Fig.…”

Section: Profiling Intact Proteins By Lc-esi-msmentioning

confidence: 97%

“…Selection of such bioactive proteins can be carried out by selecting them from the database of BC proteins recently identified by us using a proteomic and bioinformatics approach [5]. Sensitive and selective analytical methods such as ELISA are then used for their quantitative measurement.…”

Section: Targeting Bioactive Proteins By Elisa Assaymentioning

confidence: 99%

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Set-up and application of an analytical approach for the quality control of purified colostrum as food supplement

Altomare

Regazzoni

Parra

et al. 2016

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Section: Profiling Intact Proteins By Lc-esi-msmentioning

confidence: 97%

Section: Targeting Bioactive Proteins By Elisa Assaymentioning

confidence: 99%

See 2 more Smart Citations

Set-up and application of an analytical approach for the quality control of purified colostrum as food supplement

Altomare

Regazzoni

Parra

et al. 2016

Journal of Chromatography B

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“…Nano LC-MS/MS is a superior platform for protein identification. Compared to conventional mass spectrometry technology, it is more sensitive because it can identify a protein based on MS/MS sequencing even with only one peptide and is also more reliable because it can identify 1,000 protein mixtures with the same reliability as that for identifying one protein [9]. In this study, we employed nano-HPLC-MS/MS analysis to identify serum biomarkers that predict the clinical response to CNI therapy in PMN patients and validated them by Western blot and ELISA.…”

Section: Introductionmentioning

confidence: 99%

Response Predictors to Calcineurin Inhibitors in Patients with Primary Membranous Nephropathy

et al. 2018

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Background: Currently, there is an urgent need to find ways of identifying primary membranous nephropathy (PMN) patients who are likely to benefit from calcineurin inhibitors (CNI) or who are resistant to them. In this study, we employed nano-HPLC-MS/MS analysis to identify serum biomarkers that predict the clinical response to CNI therapy in PMN patients. Methods: The endpoint was complete remission (CR) after CNI treatment. PMN patients were grouped into no-remission (NR) or CR groups to screen predictive candidates using the nano-HPLC-MS/MS analysis. Results: Compared with NR patients, 3 upregulated proteins and 5 downregulated proteins were found to present a twofold change in CR patients’ serum. Serum amyloid A1 protein (SAA1) was further validated by ELISA; it was decreased in patients in the NR group compared with patients in the CR group, but SAA1 in patients in these groups was lower than in healthy controls and minimal change disease patients. The area under the receiver operating characteristic (ROC) curve of SAA1 was used to distinguish PMN NR patients from those in remission and was 0.901, with a sensitivity of 78.3% and specificity of 86.8%, similar to that of the phospholipase A2 receptor (PLA2R) antibody. Combining SAA1 with the PLA2R antibody, the area under the ROC curve was 0.956, which was higher than that of SAA1 or the PLA2R antibody alone. Conclusions: Serum SAA1 may be a candidate PMN biomarker that can be used to discriminate CNI NR cases from remission patients. The combination of SAA1 and the PLA2R antibody increases the accuracy of diagnosis.

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Liquid‐phase based separation systems for depletion, prefractionation, and enrichment of proteins in biological fluids and matrices for in‐depth proteomics analysis—An update covering the period 2014–2016

Rassi

Puangpila

2016

Electrophoresis

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This review article is an update of our previous one by C. Puangpila, E. Mayadunne, and Z. El Rassi, Electrophoresis 2015, 36, 238-252. Similarly to the previous article, this review has two main topics, including (i) proteomic sample preparation (e.g., depletion of high-abundance proteins, reduction of the protein dynamic concentration range, and enrichment of a particular subproteome), and (ii) the subsequent chromatographic and/or electrophoretic prefractionation prior to protein separation and identification by LC-MS/MS. More than 70 papers published in the period extending from mid-2014 to the present have been reviewed. Although an effort was made to yield a comprehensive review article, one may safely state that this review article is by no means thorough, and the aim was to rather provide a concise description of the latest developments in the field.

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An in depth proteomic analysis based on ProteoMiner, affinity chromatography and nano-HPLC–MS/MS to explain the potential health benefits of bovine colostrum

Cited by 19 publications

References 33 publications

Set-up and application of an analytical approach for the quality control of purified colostrum as food supplement

Set-up and application of an analytical approach for the quality control of purified colostrum as food supplement

Response Predictors to Calcineurin Inhibitors in Patients with Primary Membranous Nephropathy

Liquid‐phase based separation systems for depletion, prefractionation, and enrichment of proteins in biological fluids and matrices for in‐depth proteomics analysis—An update covering the period 2014–2016

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