Set-up and application of an analytical approach for the quality control of purified colostrum as food supplement

Altomare, Alessandra; Regazzoni, Luca; Parra, Ximena Maria Paredes; Selmin, Francesca; Rumio, Cristiano; Carini, Marina

doi:10.1016/j.jchromb.2016.06.013

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…For measurements of blood XDH levels and blood 8-OHdG levels, 5 mL blood samples were collected simultaneously and immediately centrifuged to separate the serum, which was then frozen at −80 °C until measurement of blood levels of both enzymes. An enzyme-linked immunosorbent assay (ELISA) kit (Cloud-Clone Corp. Houston, TX 77084, USA) [ 88 ] and an ELISA kit for humans (Cloud-Clone Corp. Katy, TX 77494, USA) [ 89 ] were used to measure blood XDH and 8-OHdG levels. The ELISA kit used in this study is usually less sensitive to the components present in the sample (serum) and to dilution, resulting in less variation in the assay data.…”

Section: Methodsmentioning

confidence: 99%

Clinical Significance of Elevated Xanthine Dehydrogenase Levels and Hyperuricemia in Patients with Sepsis

Matsuoka,

Yamaguchi,

Kinoshita

2023

IJMS

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Patient outcomes for severe sepsis and septic shock remain poor. Excessive oxidative stress accelerates organ dysfunction in severe acute illnesses. Uric acid (UA) is the most abundant antioxidant. We hypothesized that UA and related molecules, which play a critical role in antioxidant activity, might be markers of oxidative stress in sepsis. The study aimed to clarify the clinical significance of UA and the relationship between UA, molecules related to UA, and outcomes by measuring blood UA, xanthine dehydrogenase (XDH), and 8-hydroxy-2-deoxyguanosine (8-OHdG) levels over time. Blood UA levels in septic patients were correlated with the SOFA score (ρ = 0.36, p < 0.0001) and blood XDH levels (ρ = 0.27, p < 0.0001). Blood XDH levels were correlated with the SOFA score (ρ = 0.59, p < 0.0001) and blood 8-OHdG levels (ρ = −0.32, p < 0.0001). Blood XDH levels were persistently high in fatal cases. Blood XDH level (OR 8.84, 95% CI: 1.42–91.2, p = 0.018) was an independent factor of poor outcomes. The cutoff of blood XDH level was 1.38 ng/mL (sensitivity 92.8%, specificity 61.9%), and those 1.38 ng/mL or higher were associated with a significantly reduced survival rate (blood XDH level > 1.38 ng/mL: 23.7%, blood XDH level < 1.38 ng/mL: 96.3%, respectively, p = 0.0007). Elevated UA levels due to elevated blood XDH levels in sepsis cases may reduce oxidative stress. Countermeasures against increased oxidative stress in sepsis may provide new therapeutic strategies.

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Section: Methodsmentioning

confidence: 99%

Clinical Significance of Elevated Xanthine Dehydrogenase Levels and Hyperuricemia in Patients with Sepsis

Matsuoka,

Yamaguchi,

Kinoshita

2023

IJMS

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“…The IgG contents of the IgG-enriched and IgGdepleted fractions were then determined by size exclusion chromatography (SEC) according to the method reported by Altomare et al (2016b); SEC was performed on a Thermo Finnigan Surveyor HPLC system (ThermoFinnigan Italia, Milan, Italy) equipped with a variable wavelength detector and an auto-sampler, controlled by Xcalibur software (version 2.0.7 Thermo Fisher Scientific, Rodano, Italy). The SEC separation was performed on a 4.6-× 300-mm Phenomenex Yarra 3u SEC-3000 column (Phenomenex Inc., Torrance, CA), with a 4 × 3 mm GFC4000 pre-column (Phenomenex Inc.), by running an isocratic flow of mobile phase containing 0.1 M sodium phosphate bibasic, 0.025% sodium azide, pH 6.8, at a constant flow rate of 0.5 mL/min.…”

Section: Igg and Igm Analysis By Size Exclusion Chromatographymentioning

confidence: 99%

Colostrum from cows immunized with a veterinary vaccine against bovine rotavirus displays enhanced in vitro anti-human rotavirus activity

Civra

Altomare

Francese

et al. 2019

Journal of Dairy Science

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Human rotaviruses represent a major cause of severe diarrheal disease in infants and young children. The limited impact of oral vaccines on global estimates of rotavirus mortality and the suboptimal use of oral rehydration justify the need for alternative prophylactic and therapeutic strategies, especially for immunocompromised hosts. The protective effects of colostrum-the first milk produced during the initial 24 to 48 h after parturition-are well documented in the literature. In particular, the ingestion of hyperimmune bovine colostrum has been proposed as an alternative preventive approach against human rotavirus gastroenteritis. Although the immunization of pregnant cows with human rotavirus boosts the release of specific immunoglobulin G in bovine colostrum, it raises regulatory and safety issues. In this study, we demonstrated that the conventional bovine rotavirus vaccine is sufficient to enhance the anti-human rotavirus protective efficacy of bovine colostrum, thus providing a conservative approach to produce hyperimmune bovine colostrum, making it exploitable as a functional food.

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“…The analytical separation techniques based on chromatography and electrophoresis have been used for the separation, fractionation, and purification of the colostrum/milk components [5,[18][19][20][21][22]. For separation of IgG, SEC [23,24], affinity chromatography [24,25], and RPLC [26] have been used. Recently, mixed-mode RP chromatography has been described for isolation of IgG from bovine colostrum [27].…”

Section: Introductionmentioning

confidence: 99%

Preparative separation of immunoglobulins from bovine colostrum by continuous divergent‐flow electrophoresis

Št́astná

Šlais

2022

J of Separation Science

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Immunoglobulins in bovine colostrum were separated and fractionated from other proteins using the method and instrumentation developed in our laboratory. The proposed separation was based on bidirectional isotachophoresis/moving boundary electrophoresis with electrofocusing of the analytes in a pH gradient from 3.9 to 10.1. The preparative instrumentation included the trapezoidal non-woven fabric that served as separation space with divergent continuous flow. The defatted and casein precipitate-free colostrum supernatant was loaded directly into the instrument without any additional colostrum prepreparation. Immunoglobulin G was fractionated from other immune proteins such as bovine serum albumin, β-lactoglobulin, and α-lactalbumin, and was continuously collected in separated fractions over 3 h. The fractions were further processed, and isolated immunoglobulin G in the liquid fractions was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by re-focusing in gel isoelectric focusing. Separated immunoglobulin G was detected in seven fractions by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a gradually decreased concentration in the fractions. Re-focusing of the proteins in the fractions by gel isoelectric focusing revealed multiple separated zones of immunoglobulin G with the isoelectric point values covering the range from 5.4 to 7.2. Each fraction contained distinct zones with gradually increased isoelectric point values and decreased concentrations from fraction to fraction.

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Set-up and application of an analytical approach for the quality control of purified colostrum as food supplement

Cited by 4 publications

References 14 publications

Clinical Significance of Elevated Xanthine Dehydrogenase Levels and Hyperuricemia in Patients with Sepsis

Clinical Significance of Elevated Xanthine Dehydrogenase Levels and Hyperuricemia in Patients with Sepsis

Colostrum from cows immunized with a veterinary vaccine against bovine rotavirus displays enhanced in vitro anti-human rotavirus activity

Preparative separation of immunoglobulins from bovine colostrum by continuous divergent‐flow electrophoresis

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