A preliminary vaccine potency trial of a Newcastle Disease Virus inactivated with binary ethylenimine

Buonavoglia, Canio; Fioretti, Alessandro; Tollis, Maria; Menna, F.; Papparella, V.

doi:10.1007/bf00362801

Cited by 13 publications

(4 citation statements)

References 4 publications

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“…The IHA titers in this study were similar to those of Buonavoglia et al(1988b) with Newcastle disease virus (NDV) treated with formalin or BEI and given in an oil vaccine. They observed that the BEI-inactivated vaccine stimulated significantly higher antibody titers.…”

Section: Resultssupporting

confidence: 68%

Immunogenicity of formaldehyde and binary ethylenimine inactivated infectious bursal disease virus in broiler chicks

Habib

Hussaın

Irshad

et al. 2006

J. Zhejiang Univ. - Sci. B

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Abstract:Infectious bursal disease virus (IBDV) was inactivated by two different chemicals-formaldehyde and binary ethylenimine (BEI). Formaldehyde was used at 0.1% and 0.2%, while BEI was used at concentrations of 0.001 and 0.002 mol/L. These four vaccines were tested for their efficiency in generating humoral immune response in different groups of broiler chicks. Both BEI-inactivated vaccines gave relatively higher antibody titers and were almost twice as efficient as formaldehyde-inactivated ones.

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Section: Resultssupporting

confidence: 68%

Immunogenicity of formaldehyde and binary ethylenimine inactivated infectious bursal disease virus in broiler chicks

Habib

Hussaın

Irshad

et al. 2006

J. Zhejiang Univ. - Sci. B

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“…Pyke et al(2004) also used BEI for inactivation of different viruses. Buonavoglia et al(1988b) inactivated Newcastle disease virus (NDV) with BEI. The BEI inactivated vaccine had almost twice the efficiency when compared to a vaccine prepared with formalin.…”

Section: Discussionmentioning

confidence: 99%

Inactivation of infectious bursal disease virus by binary ethylenimine and formalin

Habib

Hussaın

Fang

et al. 2006

J. Zhejiang Univ. - Sci. B

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Abstract:In this experiment conducted to study the inactivation dynamics of infectious bursal disease virus (IBDV) by binary ethylenimine (BEI) in comparison with formalin, IBDV was isolated from the bursa of infected chickens and its confirmation was done by agar gel precipitation test. Viral suspensions were subjected to inactivation with BEI and formalin for pre-set time intervals. BEI was employed at concentrations of 0.001 and 0.002 mol/L while formalin was used at 0.1% and 0.2%. Sampling was done at 6, 12, 24, 36 and 48 h of incubation and samples were tested for their inactivation status in 9-day-old embryonated eggs and 3-week-old broiler chickens. IBDV was completely inactivated by 0.001 and 0.002 mol/L BEI after 36 h of incubation at 37 °C, whereas formalin at 0.1% and 0.2% concentrations inactivated IBDV in 24 h.

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“…This mode of action tends to "fix" the viral antigen, causing some change in immunogenicity when compared with other inactivants (27). Its inactivation activity is thought to be due to its reaction with viral proteins and nucleic acid by cross-linkage of amino groups.…”

Section: Chemical Methodsmentioning

confidence: 99%

The Production and Regulation of Veterinary Virus Vaccines

Long¹

1994

Animal Cell Biotechnology

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A preliminary vaccine potency trial of a Newcastle Disease Virus inactivated with binary ethylenimine

Abstract: Inactivation of Newcastle Disease Virus (NDV) by binary ethylenimine (BEI) is reported. The activity of an oil vaccine prepared with BEI-inactivated NDV was compared to a vaccine prepared with formalin-inactivated NDV. The BEI inactivated vaccine had almost twice the efficacy.

Cited by 13 publications

References 4 publications

Immunogenicity of formaldehyde and binary ethylenimine inactivated infectious bursal disease virus in broiler chicks

Immunogenicity of formaldehyde and binary ethylenimine inactivated infectious bursal disease virus in broiler chicks

Inactivation of infectious bursal disease virus by binary ethylenimine and formalin

The Production and Regulation of Veterinary Virus Vaccines

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