The Production and Regulation of Veterinary Virus Vaccines

Long, Rhona

doi:10.1016/b978-0-08-092599-8.50017-1

Cited by 2 publications

(1 citation statement)

References 20 publications

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“…While it has proved possible to cultivate the foot and mouth disease virus (FMD) in a baby hamster kidney (BHK) suspensionadapted cell line in sufficient quantities to satisfy the large world market for this animal vaccine (Barteling and Vreewijk, 1991), most veterinary virus vaccines remain produced in anchorage dependent cell culture systems. These cell cultivation systems still include now relatively old technology such as roller bottle culture, or multi-tray cell factories (Long 1994).…”

Section: Introductionmentioning

confidence: 99%

Untitled

Moran

1999

Cytotechnology

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Veterinary viral vaccines generally comprise either attenuated or chemically inactivated viruses which have been propagated on mammalian cell substrates or specific pathogen free (SPF) eggs. New generation vaccines include chemically inactivated virally-infected whole cell vaccines. The NM57 cell line is a bovine nasal turbinate persistently infected (non-lytic infection) with a strain of the respiratory syncytial virus (RSV). The potential of microcarrier technology for the cultivation in bioreactors of this anchorage dependent cell line for RSV vaccine production has been investigated. Both Cytodex 3 and Cultispher S microcarriers proved most suitable from a selection of microcarriers as growth substrates for this NM57 cell line. Maximum cell densities of 4.12×105 cells ml-1and 5.52×105 cells ml-1 respectively were obtained using Cytodex 3 (3 g l-1) and and Cultispher S (1 g l-1) in 5 l bioreactor cultures. The fact that cell growth was less sensitive to agitation rate when cultured on Cultispher S microcarriers, and that cells were efficiently harvested from this microcarrier by an enzymatic method, suggested Cultispher S is suitable for further evaluation at larger bioreactor scales (>5 l) than that described here.

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Section: Introductionmentioning

confidence: 99%