A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery

Ballestín, Alberto; Casado, Javier G.; Abellán, E. Doménech; Vela, Francisco Javier; Campos, Jose; Martínez-Chacón, Guadalupe; Bote, Jorge; Blázquez, Rebeca; Sánchez‐Margallo, Francisco M.

doi:10.3791/60292

Cited by 6 publications

(7 citation statements)

References 5 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The selected time for primary ischemia was 8 hours, based on previous studies and our own research experience with this animal model. 2,11,[14][15][16] Macroscopically, our study results showed a significant decrease in flap survival in the I/R control group compared with the control group (p < 0.05). These results conflicted with the histological findings as higher levels of edema formation (p < 0.05), neutrophil infiltration (p < 0.05), and structural damage were observed in the I/R group than in the control group.…”

Section: Discussionmentioning

confidence: 52%

Effects of Ischemic Preconditioning and C1 Esterase Inhibitor Administration following Ischemia-Reperfusion Injury in a Rat Skin Flap Model

Masa

Casado-Pérez

Crespo-Lora

et al. 2020

J Reconstr Microsurg

Self Cite

View full text Add to dashboard Cite

Background Ischemia-reperfusion (I/R) injury is a serious condition that can affect the success rate of microsurgical reconstructions of ischemic amputated limbs and complex tissue defects requiring free tissue transfers. The purpose of this study was to evaluate the effects of ischemic preconditioning (IPC) and C1 esterase inhibitor (C1-Inh) intravenous administration following I/R injury in a rat skin flap model. Methods Superficial caudal epigastric skin flaps (3 cm × 7 cm) were performed on 50 Wistar rats that were randomly divided into five groups. Ischemia was not induced in the control group. All other flaps underwent 8 hours of ischemia prior to revascularization: I/R control group (8-hour ischemia), IPC group (preconditioning protocol + 8-hour ischemia), C1-Inh group (8-hour ischemia + C1-Inh), and IPC + C1-Inh group (preconditioning protocol + 8-hour ischemia + C1-Inh). Survival areas were macroscopically assessed after 1 week of surgery, and histopathological and biochemical evaluations were also measured. Results There were no significant differences in flap survival between the treatment groups that were suffering 8 hours of ischemia and the control group. A significant increase in neovascularization and lower edema formation were observed in the IPC group compared with that in the I/R group. Biochemical parameters did not show any significant differences. Conclusion Intravenous administration of C1-Inh did not significantly modulate I/R-related damage in this experimental model, but further research is needed. On the other hand, IPC reduces tissue damage and improves neovascularization, confirming its potential protective effects in skin flaps following I/R injury.

show abstract

Section: Discussionmentioning

confidence: 52%

Effects of Ischemic Preconditioning and C1 Esterase Inhibitor Administration following Ischemia-Reperfusion Injury in a Rat Skin Flap Model

Masa

Casado-Pérez

Crespo-Lora

et al. 2020

J Reconstr Microsurg

Self Cite

View full text Add to dashboard Cite

show abstract

“…Since we performed the clamping, we took this event as the gold standard. The model was implemented following the technique described by Ballestin et al, 17 with a cutaneous flap based on the inferior epigastric vessels.…”

Section: Methodsmentioning

confidence: 99%

“…In this model, we did not dissect the pedicles adventitia to avoid spasm or perfusion bias. 17 However, we did skeletonize the pedicle to identify it and to ensure pedicle occlusion with the clamp. The flap was placed in a rigid nylon mesh with squares of 5 × 7 cm to avoid curling, and was secured to that mesh using nylon simple sutures.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Predictive Values of an Automatic Device Measuring Oximetry in Free Flaps

Rendón-Medina

Galeana-Pavón

Vázquez-Medina

et al. 2021

Plastic and Reconstructive Surgery - Global Open

View full text Add to dashboard Cite

Summary: Free-flap monitoring is challenging to perform in some centers. It requires the availability of trained health care personnel for 24 hours a day and seven days a week. Many methods had been proposed for flap monitoring, and none of them are superior to clinical evaluation. This study aimed to present a murine model to evaluate the accuracy (sensitivity, specificity, and the positive or negative predictive values) of a device. Wistar rats weighing 240–490 g were included for intervention and data collection. A murine model of left inferior epigastric vessel flaps was implemented. Intermittent pedicle clamping was performed to calculate the accuracy of the device that detects flow obstruction. The general variables studied were age, weight, and gender. The sensitivity, specificity, and negative or predictive values were calculated. The results showed a sensitivity of 97%, a specificity of 95% with a positive predictive value of 95%, and negative predictive value of 97%. The sensitivity and specificity showed excellent results within the range of clinical security. We require more data to analyze the multiparameter monitoring to see if it is feasible and cost-effective.

show abstract

“…In relation to robotic microsurgery teaching, new simulators and specific models are being developed for training. But some of the models already described for reconstructive microsurgery training and research [18][19][20][21][22] are also useful, especially those based on the rat epigastric skin free flap (groin flap) in rats. This flap is perfused by the caudal epigastric artery (0.35 mm diameter) and the caudal epigastric vein (0.70 mm diameter), thus, it is a good model in which to perform suprermicrosurgical anastomoses with 11/0 and 12/0 sutures.…”

Section: Supermicrosurgery and Robotic Microsurgerymentioning

confidence: 99%

Microsurgery education in Spain

Ballestín

Cuadros²

2021

ВХ

View full text Add to dashboard Cite

Prior to microsurgery practice on patients, microsurgical skills should be learned in a simulated and controlled environment to increase success rates and reduce surgical complications. These favorable environments have historically been the microsurgery laboratories. The use of the experimental animals has allowed surgical trainees to interact with anatomical structures and physiological processes that a microsurgeon has to face in daily clinical scenarios. In recent decades, there has been an increase in simulation methods to reduce the number of animals used for training purposes and thus meet animal welfare criteria.Spain has a long history in the practice and teaching of microsurgery, this manuscript aims to highlight the importance of first educators, as well as to evaluate the current situation and future perspectives.

show abstract

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery

Cited by 6 publications

References 5 publications

Effects of Ischemic Preconditioning and C1 Esterase Inhibitor Administration following Ischemia-Reperfusion Injury in a Rat Skin Flap Model

Effects of Ischemic Preconditioning and C1 Esterase Inhibitor Administration following Ischemia-Reperfusion Injury in a Rat Skin Flap Model

Evaluation of Predictive Values of an Automatic Device Measuring Oximetry in Free Flaps

Microsurgery education in Spain

Contact Info

Product

Resources

About