A potential reusable fluorescent aptasensor based on magnetic nanoparticles for ochratoxin A analysis

Qin, Pin-Zhu; Huang, Da-Wei; Xu, Zihao; Guan, Ying; Bing, Yongxin; Yu, Ang

doi:10.1515/chem-2019-0140

Cited by 5 publications

(1 citation statement)

References 39 publications

(32 reference statements)

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“…Another advantage of magnetic separation is removal of sample components that could affect subsequent fluorescence detection. There are many publications on IFA developed using MNPs (Godjevargova et al 2019;Ivanov et al 2019;Qin et al 2019). Usually the MNPs are used for extraction of OTA, and its detection is performed with different analytical tools (Sargazi and Majd 2017).…”

Section: Introductionmentioning

confidence: 99%

Magnetic Nanoparticle-Based Fluorescence Immunoassay for Determination of Ochratoxin A in Milk

et al. 2020

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Ochratoxins are possible human carcinogens. The aim of this study is to develop a rapid and sensitive competitive immunofluorescent analysis for determination of ochratoxin A (OTA) on the base of immobilized polyclonal antibody against ochratoxin and immobilized F(ab′) 2 fragment on magnetic nanoparticles (MNPs). F(ab′) 2 fragment of anti-OTA antibody was obtained by pepsin hydrolysis of polyclonal antibody against OTA. The competitive fluorescent conjugate OTA-OVA-FITC (OTA coupled to ovalbumin (OVA) and then conjugated to fluorescein isothiocyanate (FITC)) was prepared and purified by size-exclusion chromatography. Competitive immunoassay was performed by using obtained immobilized antibody or F(ab′) 2 fragment on magnetic nanoparticles and the conjugate OTA-OVA-FITC. The analytical characteristics of the analysis with immobilized polyclonal antibody and F(ab′) 2 fragment were compared. The linear measuring range of OTA in milk, obtained with immobilized whole antibody, was from 0.1 to 2.5 ng/mL OTA and with immobilized F(ab′) 2 fragment from 0.1 to 7.5 ng/mL OTA. The detection limit of immunoassay with immobilized whole antibody was 0.1 ng/mL OTA and with immobilized F(ab′) 2 fragment was 0.08 ng/mL OTA. Milk samples were spiked with OTA at different levels. The recovery rates when using immobilized F(ab′) 2 fragment were between 99.4 and 118.0%, and the relative standard deviations (RSDs) were 6.5-7.7%. The results indicate that this fluorescence immunoassay with MNPs was accurate and has good reproducibility.

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Section: Introductionmentioning

confidence: 99%

Magnetic Nanoparticle-Based Fluorescence Immunoassay for Determination of Ochratoxin A in Milk

et al. 2020

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Recent trends in fluorescent aptasensors for mycotoxin detection in food: Principles, constituted elements, types, and applications

Yan

Chen

et al. 2022

Food Frontiers

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Mycotoxins are the secondary metabolites of fungi in food and can be toxic to people. Therefore, developing simple, sensitive, and specific detection methods to detect mycotoxins in food is essential for ensuring food safety. Fluorescent aptasensors for the detection of mycotoxins in food have developed rapidly. They are simple and fast with high affinity and specificity. However, fluorescent aptasensors have not yet been applied in officially approved standards so far. This review elucidates fluorescent aptasensors for mycotoxin detection, including principles, constituted elements, types, and applications. Screening results of aptamers and each aptamer sequence used in fluorescent aptasensors are summarized to facilitate further research for later studies.We also focused on fluorescent probes, nanomaterials, and other strategies in the process of constructing fluorescent aptasensors. We then discuss current challenges and future perspectives in fluorescent aptasensors for mycotoxin detection.

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Nanomaterial-based aptamer biosensors for ochratoxin A detection: a review

et al. 2022

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A potential reusable fluorescent aptasensor based on magnetic nanoparticles for ochratoxin A analysis

Cited by 5 publications

References 39 publications

Magnetic Nanoparticle-Based Fluorescence Immunoassay for Determination of Ochratoxin A in Milk

Magnetic Nanoparticle-Based Fluorescence Immunoassay for Determination of Ochratoxin A in Milk

Recent trends in fluorescent aptasensors for mycotoxin detection in food: Principles, constituted elements, types, and applications

Nanomaterial-based aptamer biosensors for ochratoxin A detection: a review

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