A portable, 3D printed, microfluidic device for multiplexed, real time, molecular detection of the porcine epidemic diarrhea virus, transmissible gastroenteritis virus, and porcine deltacoronavirus at the point of need

El‐Tholoth, Mohamed; Bai, Huiwen; Mauk, Michael G.; Saif, Linda J.; Bau, Haim H.

doi:10.1039/d0lc01229g

Cited by 36 publications

(28 citation statements)

References 42 publications

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“…Our custom microfluidic chip (28 mm × 28 mm × 4 mm) consists of four 20 µL independent multifunctional, isothermal amplification reactors (MIAR). The microfluidic chip was designed with SolidWorks 2020 (DS SolidWorks TM , Waltham, MA, USA), fabricated by a Low Force Stereolithography (LFS) 3D printer Form 3 (Formlabs TM , Somerville, MA, USA) using a clear resin FLGPCL04 (Formlabs TM , Somerville, MA, USA), and coated with polyethylene glycol (PEG) 3350 aqueous solution (2%) [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip

El‐Tholoth

Bai

Mauk

et al. 2021

Animals

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Infectious laryngotracheitis (ILT) is a viral disease of chickens’ respiratory system that imposes considerable financial burdens on the chicken industry. Rapid, simple, and specific detection of this virus is crucial to enable proper control measures. Polymerase chain reaction (PCR)-based molecular tests require relatively expensive instruments and skilled personnel, confining their application to centralized laboratories. To enable chicken farms to take timely action and contain the spread of infection, we describe a rapid, simple, semi-quantitative benchtop isothermal amplification (LAMP) assay, and a field-deployable microfluidic device for the diagnosis of ILTV infection in chickens. Our assay performance was compared and favorably agreed with quantitative PCR (qPCR). The sensitivity of our real-time LAMP test is 250 genomic copies/reaction. Clinical performance of our microfluidic device using samples from diseased chickens showed 100% specificity and 100% sensitivity in comparison with benchtop LAMP assay and the gold-standard qPCR. Our method facilitates simple, specific, and rapid molecular ILTV detection in low-resource veterinary diagnostic laboratories and can be used for field molecular diagnosis of suspected ILT cases.

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Section: Methodsmentioning

confidence: 99%

Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip

El‐Tholoth

Bai

Mauk

et al. 2021

Animals

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“…El-Tholoth et al. ( 2021 ) designed a new, low-cost, rapid, semi-quantitative, field deployable, 3 D-printed microfluidic device for auto-distribution of samples and self-sealing, as well as real-time and reverse transcription-loop-mediated isothermal amplification (RT-LAMP), allowing the co-identification of PEDV, TGEV, and PDCoV in around 30 minutes.…”

Section: Porcine Covs: Pathogenesis and Clinical Signsmentioning

confidence: 99%

Swine coronaviruses (SCoVs) and their emerging threats to swine population, inter-species transmission, exploring the susceptibility of pigs for SARS-CoV-2 and zoonotic concerns

Thakor

Dinesh

Manikandan

et al. 2022

Veterinary Quarterly

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Swine coronaviruses (SCoVs) are one of the most devastating pathogens affecting the livelihoods of farmers and swine industry across the world. These include transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), porcine respiratory coronavirus (PRCV), porcine hemagglutinating encephalomyelitis virus (PHEV), swine acute diarrhea syndrome coronavirus (SADS-CoV), and porcine delta coronavirus (PDCoV). Coronaviruses infect a wide variety of animal species and humans because these are having single stranded-RNA that accounts for high mutation rates and thus could break the species barrier. The gastrointestinal, cardiovascular, and nervous systems are the primary organ systems affected by SCoVs. Infection is very common in piglets compared to adult swine causing high mortality in the former. Bat is implicated to be the origin of all CoVs affecting animals and humans. Since pig is the only domestic animal in which CoVs cause a wide range of diseases; new coronaviruses with high zoonotic potential could likely emerge in the future as observed in the past. The recently emerged severe acute respiratory syndrome coronavirus virus-2 (SARS-CoV-2), causing COVID-19 pandemic in humans, has been implicated to have animal origin, also reported from few animal species, though its zoonotic concerns are still under investigation. This review discusses SCoVs and their epidemiology, virology, evolution, pathology, wildlife reservoirs, interspecies transmission, spill-over events and highlighting their emerging threats to swine population. The role of pigs amid ongoing SARS-CoV-2 pandemic will also be discussed. A thorough investigation should be conducted to rule out zoonotic potential of SCoVs and to design appropriate strategies for their prevention and control.

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“…The following supporting information can be downloaded at: https:// www.mdpi.com/article/10.3390/v14071355/s1, Figure S1 S1: Quality Assessment of Diagnostic Accuracy Studies 2 (QUADAS-2) summary items for risk of bias (A) and applicability concerns (B) for all studies. References [18,20,22,26,29,30,33,[36][37][38][39][40] are cited in the supplementary materials.…”

Section: Supplementary Materialsmentioning

confidence: 99%

Point-of-Care Tests for Rapid Detection of Porcine Epidemic Diarrhea Virus: A Systematic Review and Meta-Analysis

Tian

Pang

et al. 2022

Viruses

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The timely and accurate diagnosis of porcine epidemic diarrhea virus (PEDV) infection is crucial to reduce the risk of viral transmission. Therefore, the objective of this review was to evaluate the overall diagnostic accuracy of rapid point-of-care tests (POCTs) for PEDV. Studies published before 7 January 2022 were identified by searching PubMed, EMBASE, Springer Link, and Web of Science databases, using subject headings or keywords related to point of care and rapid test diagnostic for PEDV and PED. Two investigators independently extracted data, rated risk of bias, and assessed the quality using the Quality Assessment of Diagnostic Accuracy Studies-2 tool. The bivariate model and the hierarchical summary receiver operating characteristic (HSROC) model were used for performing the meta-analysis. Threshold effect, subgroup analysis, and meta-regression were applied to explore heterogeneity. Of the 2908 records identified, 24 eligible studies involving 3264 specimens were enrolled in the meta-analysis, including 11 studies on evaluation of lateral flow immunochromatography assay (ICA)-based, and 13 on nucleic acid isothermal amplification (NAIA)-based POCTs. The overall pooled sensitivity, specificity and diagnostic odds ratio (DOR) were 0.95 (95% CI: 0.92–0.97), 0.96 (95% CI 0.88–0.99) and 480 (95% CI 111–2074), respectively; for ICA-based POCTs and the corresponding values for NAIA-based, POCTs were 0.97 (95% CI 0.94–0.99), 0.98 (95% CI 0.91–0.99) and 1517 (95% CI 290–7943), respectively. The two tests showed highly comparable and satisfactory diagnostic performance in clinical utility. These results support current recommendations for the use of rapid POC tests when PEDV is suspected.

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A portable, 3D printed, microfluidic device for multiplexed, real time, molecular detection of the porcine epidemic diarrhea virus, transmissible gastroenteritis virus, and porcine deltacoronavirus at the point of need

Abstract: The porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine deltacoronavirus (PDCoV) are coronaviruses (CoVs) of neonatal pigs that cause great economic losses to pig farms and pork processors.

Cited by 36 publications

References 42 publications

Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip

Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip

Swine coronaviruses (SCoVs) and their emerging threats to swine population, inter-species transmission, exploring the susceptibility of pigs for SARS-CoV-2 and zoonotic concerns

Point-of-Care Tests for Rapid Detection of Porcine Epidemic Diarrhea Virus: A Systematic Review and Meta-Analysis

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