This review summarizes the recent advances and the main strategies to improve the sensitivity of amplification-free CRISPR/Cas-based detection techniques.
The porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine deltacoronavirus (PDCoV) are coronaviruses (CoVs) of neonatal pigs that cause great economic losses to pig farms and pork processors.
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