A point-of-use microfluidic device with integrated photodetector array for immunoassay multiplexing: Detection of a panel of mycotoxins in multiple samples

Soares, Ruben R. G.; Santos, Denis R.; Chu, V.; Azevedo, Ana M.; Aires-Barros, M. Raquel; Conde, J.P.

doi:10.1016/j.bios.2016.09.041

Cited by 44 publications

(14 citation statements)

References 19 publications

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“…To decrease the complexity of the assay which used multiwavelength or light scattering for quantification, in 2017, Soares et al developed a novel integrated device with manually operated magnetic valves for the detection of ochratoxin A (OTA), aflatoxin B1 (AFB1), and deoxynivalenol (DON). e LOD of these three kinds of fungi was 100 ng/mL, 100 ng/mL, and 3 ng/mL, respectively [63]. In some conventional optimized magneto-immunoassays [77,78], fluorescent [79] and magnetic beads (MB) incubation are conveyed under agitation and MB washings rely on magnets, which hinder the integration with a low-cost POC device.…”

Section: Magnetic Nondigital Microfluidic System Inmentioning

confidence: 99%

Application of Microfluidics in Immunoassay: Recent Advancements

Shi

Peng

Yang

et al. 2021

Journal of Healthcare Engineering

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In recent years, point-of-care testing has played an important role in immunoassay, biochemical analysis, and molecular diagnosis, especially in low-resource settings. Among various point-of-care-testing platforms, microfluidic chips have many outstanding advantages. Microfluidic chip applies the technology of miniaturizing conventional laboratory which enables the whole biochemical process including reagent loading, reaction, separation, and detection on the microchip. As a result, microfluidic platform has become a hotspot of research in the fields of food safety, health care, and environmental monitoring in the past few decades. Here, the state-of-the-art application of microfluidics in immunoassay in the past decade will be reviewed. According to different driving forces of fluid, microfluidic platform is divided into two parts: passive manipulation and active manipulation. In passive manipulation, we focus on the capillary-driven microfluidics, while in active manipulation, we introduce pressure microfluidics, centrifugal microfluidics, electric microfluidics, optofluidics, magnetic microfluidics, and digital microfluidics. Additionally, within the introduction of each platform, innovation of the methods used and their corresponding performance improvement will be discussed. Ultimately, the shortcomings of different platforms and approaches for improvement will be proposed.

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Section: Magnetic Nondigital Microfluidic System Inmentioning

confidence: 99%

Application of Microfluidics in Immunoassay: Recent Advancements

Shi

Peng

Yang

et al. 2021

Journal of Healthcare Engineering

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“…Simultaneous detection of multiple mycotoxins by a microfluidic a-Si:H photosensors was also realized. Soares et al [ 117 ] developed a microfluidic multiplexed immunochip utilizing a permanent magnet valve and a single negative pressure source for the simultaneous detection of OTA, AFB1, and DON (three mycotoxins) by the integrated a-Si:H photodetector. Three mycotoxins of 100 μg/L for OTA and DON, and 3 μg/L for AFB1 were detected in less than 20 min.…”

Section: Application Of Microchips For the Determination Of Mycotomentioning

confidence: 99%

Recent Advances in Mycotoxin Determination for Food Monitoring via Microchip

Man

Liang

Liu

et al. 2017

Toxins

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Mycotoxins are one of the main factors impacting food safety. Mycotoxin contamination has threatened the health of humans and animals. Conventional methods for the detection of mycotoxins are gas chromatography (GC) or liquid chromatography (LC) coupled with mass spectrometry (MS), or enzyme-linked immunosorbent assay (ELISA). However, all these methods are time-consuming, require large-scale instruments and skilled technicians, and consume large amounts of hazardous regents and solvents. Interestingly, a microchip requires less sample consumption and short analysis time, and can realize the integration, miniaturization, and high-throughput detection of the samples. Hence, the application of a microchip for the detection of mycotoxins can make up for the deficiency of the conventional detection methods. This review focuses on the application of a microchip to detect mycotoxins in foods. The toxicities of mycotoxins and the materials of the microchip are firstly summarized in turn. Then the application of a microchip that integrates various kinds of detection methods (optical, electrochemical, photo-electrochemical, and label-free detection) to detect mycotoxins is reviewed in detail. Finally, challenges and future research directions in the development of a microchip to detect mycotoxins are previewed.

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“…The content of substances is proportional to the intensity of the luminous signal. It can realize sensitive detecting of trace markers in samples and be used as a conventional detector in microfluidic chips ( Hu et al, 2017 ; Soares et al, 2017 ; Xie et al, 2019 ). A biochip with 3 × 8 wells array was constructed to simultaneously detect three cardiac markers cTnI, CK-MB, and Myo ( Zhao et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Point-of-Care Testing for Multiple Cardiac Markers Based on a Snail-Shaped Microfluidic Chip

et al. 2021

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Existing methods for detecting cardiac markers are difficult to be applied in point-of-care testing (POCT) due to complex operation, long time consumption, and low sensitivity. Here, we report a snail-shaped microfluidic chip (SMC) for the multiplex detection of cTnI, CK-MB, and Myo with high sensitivity and a short detection time. The SMC consists of a sandwich structure: a channel layer with a mixer and reaction zone, a reaction layer coated with capture antibodies, and a base layer. The opening or closing of the microchannels is realized by controlling the downward movement of the press-type mechanical valve. The chemiluminescence method was used as a signal readout, and the experimental conditions were optimized. SMC could detect cTnI, CK-MB, and Myo at concentrations as low as 1.02, 1.37, and 4.15. The SMC will be a promising platform for a simultaneous determination of multianalytes and shows a potential application in POCT.

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A point-of-use microfluidic device with integrated photodetector array for immunoassay multiplexing: Detection of a panel of mycotoxins in multiple samples

Cited by 44 publications

References 19 publications

Application of Microfluidics in Immunoassay: Recent Advancements

Application of Microfluidics in Immunoassay: Recent Advancements

Recent Advances in Mycotoxin Determination for Food Monitoring via Microchip

Point-of-Care Testing for Multiple Cardiac Markers Based on a Snail-Shaped Microfluidic Chip

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