A pilot study of the validation of percutaneous testing in cats

Abstract

Percutaneous application of 6 mg/mL glycerinated histamine solution, 50% glycerosaline solution and 0.9% buffered saline produced similar positive and negative control wheals. These observations warrant further studies of percutaneous allergen testing in cats with hypersensitivity dermatitis.

Percutaneous prick test irritant threshold concentrations for eight allergens in healthy nonsedated dogs in the USA

“…Another study investigated several PT variables in cats using the GREER ® Pick ® System (Stallergenes Greer; Lenoir, NC, USA). Glycerinated histamine (6 mg/ml) and glycerosaline were found to be reliable positive and negative controls, respectively . Another study compared irritant reactions of plants, insects, canine dander and moulds using IDT and PT in healthy cats.…”

Comparison of intradermal and percutaneous testing to histamine, saline and nine allergens in healthy adult cats

“…Similar to the previous study, the PG histamine wheals were well demarcated and easy to evaluate. 11 A canine study also found that percutaneously applied 5 mg/mL histamine created well-demarcated wheals. 14 PA histamine wheals were poorly visible and PA testing with 0.0275 mg/mL aqueous histamine is therefore not recommended.…”

“…11 The groups were not compared statistically. In the current study, the PG and ID wheals were compared statistically, with the ID histamine wheals being consistently larger.…”

“…That study evaluated histamine, saline and glycerosaline reactions using two different skin picks at 15, 20 and 25 min. 11 The GREER â Pick System (GREER â ; Lenoir, NC, USA) produced reliable and well-demarcated wheals with 6 mg/mL glycerinated histamine as a positive control without creating wheals with the negative glycerosaline control. In contrast, the Duotip-test II pick â (Lincoln Diagnostics Inc.; Decatur, IL, USA) glycerinated histamine reactions were small and not significantly different from glycerosaline.…”

“…Compared to the histamine wheals created by IDT, the histamine wheals made using the GREER â Pick â System were better demarcated, indicating that PCT may be used as an alternative to IDT in the cat. 11 Our study compared PCT and IDT in healthy cats using nine allergens as well as histamine and saline controls. The primary goal was to determine if percutaneously applied aqueous and glycerinated allergens would create irritant reactions in healthy domestic cats using the GREER â Pick â System.…”