Comparison of intradermal and percutaneous testing to histamine, saline and nine allergens in healthy adult cats

Abstract

Percutaneously (PCT) applied allergens did not cause irritant reactions in healthy cats. PG histamine wheals, although smaller than ID histamine wheals, were easily recognizable and PCT was simple to perform.

Clinical signs and diagnosis of feline atopic syndrome: detailed guidelines for a correct diagnosis

“…However, a few feline studies have shown that for ≤15 allergens (grass, weed and tree pollens), the concentrations currently used for dogs would be suboptimal, partially explaining the low reactivity in cats. [91][92][93] Likewise, using healthy cats, the optimal concentration of histamine was determined to be 1:50,000 w/v instead of 1:10,000 w/v or 1:100,000 w/v used in dogs. 3,92 Because cats typically demonstrate weak (transient, small) reactions to IDT, the use of intravenously injected dye solutions (Evans blue and fluorescein) has been suggested.…”

“…undiluted). 93,98 Once the patient is sedated, the lateral thorax is clipped and the sites for the "pricks" are marked. Then, a drop (0.05 mL) of the allergen (or control) is applied and introduced into the skin via the prick device.…”

“…In dogs, the first study 99 comparing IDT and PPT showed that IDT was much easier to interpret than PPT, and thus for more than two decades the PPT was abandoned. New tools have been patented to administer the allergens in a more consistent and standardised fashion, and the PPT has been rediscovered first in cats 93,100 and subsequently in dogs 98 . In particular, reliable controls (6 mg/mL glycerinated histamine and 50% glycerosaline solution) have been identified in cats 100 …”

Percutaneous prick test irritant threshold concentrations for eight allergens in healthy nonsedated dogs in the USA

“…Another study compared irritant reactions of plants, insects, canine dander and moulds using IDT and PT in healthy cats. An irritant reaction to burningbush ( Bassia scoparia) with IDT, but not to any percutaneously tested allergens was reported . A fourth study investigated optimal threshold concentrations of eight allergens including plants, dust mites and storage mites using PT in dogs.…”

Clinical efficacy of sublingual allergen‐specific immunotherapy in 22 cats with atopic dermatitis

“…Despite intradermal testing (IDT) being regarded as the gold standard for allergen formulation in ASIT for many years, the serological test was used to reduce interobserver variability inherent in IDT, over the one year study. 28,29 The IgE serological test used contains the alpha chain of the Fc-epsilon receptor (FceR1a) that binds IgE with very high affinity (10 À10 M). Other types of allergen-reactive antibodies, such as IgG, are not able to bind to this receptor.…”