A photoacoustic FTIRS study of the chemical modifications of human dentin surfaces: I. Demineralization

Renzo, M. Di; Ellis, T. H.; Sacher, E.; Stangel, I.

doi:10.1016/s0142-9612(00)00240-4

Cited by 47 publications

(39 citation statements)

References 25 publications

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“…This profoundly increases the surface area and exposes more carbonyl groups. As a result, there is an increase in the FTIR signal on demineralized dentin [32]. In this study, the log [amide I: HPO 4 2- ] of the control group increased over time, suggesting that more collagen I was exposed and less HPO 4 2- remained in the dentin surface.…”

Section: Discussionmentioning

confidence: 53%

Antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries

Mei

Chu

et al. 2013

Ann Clin Microbiol Antimicrob

191

154

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BackgroundsSilver diamine fluoride (SDF) has clinical success in arresting dentin caries, this study aimed to investigate its mechanism of action.MethodsUsing a computer-controlled artificial mouth, we studied the effect of 38% SDF on cariogenic biofilms and dentin carious lesions. We used five common cariogenic bacteria (Streptococcus mutans, Streptococcus sobrinus, Lactobacillus acidophilus, Lactobacillus rhamnosus and Actinomyces naeslundii) to form a cariogenic biofilm that generated carious lesions with a depth of approximately 70 um on human dentin blocks. We applied 38% SDF to the lesions in the test group and water to those in the control group. The blocks were incubated in the artificial mouth for 21 days before evaluation. Microbial kinetics, architecture, viability and distribution were evaluated every 7 days using colony forming unit (CFU), scanning electron microscopy and confocal laser scanning microscopy. The physical properties of the carious lesions were evaluated with microhardness testing, energy dispersive spectroscopy (EDS) and Fourier transform infra-red spectroscopy (FTIR).ResultsThe CFU results revealed fewer colony forming units in the test group compared with the control group (p < 0.01). Scanning electron microscopy and confocal microscopy showed less bacterial growth in the test group, and confluent cariogenic biofilm in the control group (p < 0.01). The microhardness and weight percentages of calcium and phosphorus in the test group from the outermost 50mum were higher than in the control group (p < 0.05). EDS showed that calcium and phosphous were higher in outer 50 mum in test groups than in the control FTIR revealed less exposed collagen I in the test lesions compared with the control group (p < 0.01).Conclusions38% SDF inhibits multi-species cariogenic biofilm formation on dentin carious lesions and reduces the demineralization process.

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Section: Discussionmentioning

confidence: 53%

Antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries

Mei

Chu

et al. 2013

Ann Clin Microbiol Antimicrob

191

154

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“…Both of them cause demineralization of dentin, which exposes the collagen (26,27) and contributes to create an ideal substrate for E. faecalis adherence (28). Furthermore, the greater residual activity of MA as compared with EDTA can be attributed to its stronger antimicrobial action (11,18).…”

Section: Discussionmentioning

confidence: 99%

Residual and Antimicrobial Activity of Final Irrigation Protocols on Enterococcus Faecalis Biofilm in Dentin

Baca

Junco

Arias-Moliz

et al. 2011

Journal of Endodontics

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“…This occurrence can be attributed to the extended time of acid contact with the roots (3 min). It may produce an insoluble form of that salt, which precipitates, blocking tubule openings and annulling its demineralizing effect 10,21 . As the superficial composition of these deposits was not characterized in this study, this interpretation is speculative.…”

Section: Discussionmentioning

confidence: 99%

Comparison among four commonly used demineralizing agents for root conditioning: a scanning electron microscopy

Amaral¹,

Rezende

Hirata

et al. 2011

J. Appl. Oral Sci.

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Dental roots that have been exposed to the oral cavity and periodontal pocket environment present superficial changes, which can prevent connective tissue reattachment. Demineralizing agents have been used as an adjunct to the periodontal treatment aiming at restoring the biocompatibility of roots.ObjectiveThis study compared four commonly used demineralizing agents for their capacity of removing smear layer and opening dentin tubules. MethodsFifty fragments of human dental roots previously exposed to periodontal disease were scaled and randomly divided into the following groups of treatment: 1) CA: demineralization with citric acid for 3 min; 2) TC-HCl: demineralization with tetracycline-HCl for 3 min; 3) EDTA: demineralization with EDTA for 3 min; 4) PA: demineralization with 37% phosphoric acid for 3 min; 5)Control: rubbing of saline solution for 3 min. Scanning electron microscopy was used to check for the presence of residual smear layer and for measuring the number and area of exposed dentin tubules. ResultsSmear layer was present in 100% of the specimens from the groups PA and control; in 80% from EDTA group; in 33.3% from TC-HCl group and 0% from CA group. The mean numbers of exposed dentin tubules in a standardized area were: TC-HCl=43.8±25.2; CA=39.3±37; PA=12.1±16.3; EDTA=4.4±7.5 and Control=2.3±5.7. The comparison showed significant differences between the following pairs of groups: TC-HCl and Control; TC-HCl and EDTA; CA and Control; and CA and EDTA. The mean percentages of area occupied by exposed dentin tubules were: CA=0.12±0.17%; TC-HCl=0.08±0.06%; PA=0.03±0.05%; EDTA=0.01±0.01% and Control=0±0%. The CA group differed significantly from the others except for the TC-HCl group. ConclusionThere was a decreasing ability for smear layer removal and dentin tubule widening as follows: AC>TC-HCl>PA>EDTA. This information can be of value as an extra parameter for choosing one of them for root conditioning.

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A photoacoustic FTIRS study of the chemical modifications of human dentin surfaces: I. Demineralization

Cited by 47 publications

References 25 publications

Antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries

Antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries

Residual and Antimicrobial Activity of Final Irrigation Protocols on Enterococcus Faecalis Biofilm in Dentin

Comparison among four commonly used demineralizing agents for root conditioning: a scanning electron microscopy

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